Variations in Intracellular Organometallic Reaction Frequency Captured by Single-Molecule Fluorescence Microscopy.
Allylcarbamate Cleavage
Bioorganometallic
Bioorthogonal
Intracellular Catalysis
Ruthenium
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
01 08 2023
01 08 2023
Historique:
received:
10
01
2023
pmc-release:
01
08
2024
medline:
29
9
2023
pubmed:
7
6
2023
entrez:
7
6
2023
Statut:
ppublish
Résumé
Studies of organometallic reactions in living cells commonly rely on ensemble-averaged measurements, which can obscure the detection of reaction dynamics or location-specific behavior. This information is necessary to guide the design of bioorthogonal catalysts with improved biocompatibility, activity, and selectivity. By leveraging the high spatial and temporal resolution of single-molecule fluorescence microscopy, we have successfully captured single-molecule events promoted by Ru complexes inside live A549 human lung cells. By observing individual allylcarbamate cleavage reactions in real-time, our results revealed that they occur with greater frequency inside the mitochondria than in the non-mitochondria regions. The estimated turnover frequency of the Ru complexes was at least 3-fold higher in the former than the latter. These results suggest that organelle specificity is a critical factor to consider in intracellular catalyst design, such as in developing metallodrugs for therapeutic applications.
Identifiants
pubmed: 37285476
doi: 10.1002/anie.202300467
pmc: PMC10526727
mid: NIHMS1909161
doi:
Substances chimiques
Ruthenium
7UI0TKC3U5
Organometallic Compounds
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300467Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM129276
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM133505
Pays : United States
Informations de copyright
© 2023 Wiley-VCH GmbH.
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