PlmCas12e (CasX2) cleavage of CCR5: impact of guide RNA spacer length and PAM sequence on cleavage activity.


Journal

RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328

Informations de publication

Date de publication:
01 2023
Historique:
medline: 9 6 2023
pubmed: 8 6 2023
entrez: 8 6 2023
Statut: ppublish

Résumé

Gene editing using CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR-associated) is under development as a therapeutic tool for the modification of genes in eukaryotic cells. While much effort has focused on CRISPR/Cas9 systems from

Identifiants

pubmed: 37287312
doi: 10.1080/15476286.2023.2221510
pmc: PMC10251783
doi:

Substances chimiques

RNA 63231-63-0
Nucleotides 0
CCR5 protein, human 0
Receptors, CCR5 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

296-305

Subventions

Organisme : BLRD VA
ID : I01 BX005248
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK117469
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA023108
Pays : United States

Commentaires et corrections

Type : UpdateOf

Références

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Auteurs

David A Armstrong (DA)

Research Service, V.A. Medical Center, White River Junction, VT, USA.
Departments of Dermatology, Dartmouth Health, Lebanon, NH, USA.
Departments of Dermatology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Taylor R Hudson (TR)

Research Service, V.A. Medical Center, White River Junction, VT, USA.

Christine A Hodge (CA)

Departments of Dermatology, Dartmouth Health, Lebanon, NH, USA.
Departments of Dermatology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Thomas H Hampton (TH)

Departments of Microbiology/Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Alexandra L Howell (AL)

Research Service, V.A. Medical Center, White River Junction, VT, USA.
Departments of Microbiology/Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Departments of Medicine, Dartmouth Health, Lebanon, NH, USA.

Matthew S Hayden (MS)

Departments of Dermatology, Dartmouth Health, Lebanon, NH, USA.
Departments of Dermatology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

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Classifications MeSH