Haemophilus influenzae and Moraxella catarrhalis in sputum of severe asthma with inflammasome and neutrophil activation.
Haemophilus influenzae
Moraxella catarrhalis
Tropheryma whipplei
severe asthma
α-diversity
Journal
Allergy
ISSN: 1398-9995
Titre abrégé: Allergy
Pays: Denmark
ID NLM: 7804028
Informations de publication
Date de publication:
11 2023
11 2023
Historique:
revised:
19
04
2023
received:
28
12
2022
accepted:
24
04
2023
medline:
13
11
2023
pubmed:
8
6
2023
entrez:
8
6
2023
Statut:
ppublish
Résumé
Because of altered airway microbiome in asthma, we analysed the bacterial species in sputum of patients with severe asthma. Whole genome sequencing was performed on induced sputum from non-smoking (SAn) and current or ex-smoker (SAs/ex) severe asthma patients, mild/moderate asthma (MMA) and healthy controls (HC). Data were analysed by asthma severity, inflammatory status and transcriptome-associated clusters (TACs). α-diversity at the species level was lower in SAn and SAs/ex, with an increase in Haemophilus influenzae and Moraxella catarrhalis, and Haemophilus influenzae and Tropheryma whipplei, respectively, compared to HC. In neutrophilic asthma, there was greater abundance of Haemophilus influenzae and Moraxella catarrhalis and in eosinophilic asthma, Tropheryma whipplei was increased. There was a reduction in α-diversity in TAC1 and TAC2 that expressed high levels of Haemophilus influenzae and Tropheryma whipplei, and Haemophilus influenzae and Moraxella catarrhalis, respectively, compared to HC. Sputum neutrophils correlated positively with Moraxella catarrhalis and negatively with Prevotella, Neisseria and Veillonella species and Haemophilus parainfluenzae. Sputum eosinophils correlated positively with Tropheryma whipplei which correlated with pack-years of smoking. α- and β-diversities were stable at one year. Haemophilus influenzae and Moraxella catarrhalis were more abundant in severe neutrophilic asthma and TAC2 linked to inflammasome and neutrophil activation, while Haemophilus influenzae and Tropheryma whipplei were highest in SAs/ex and in TAC1 associated with highest expression of IL-13 type 2 and ILC2 signatures with the abundance of Tropheryma whipplei correlating positively with sputum eosinophils. Whether these bacterial species drive the inflammatory response in asthma needs evaluation.
Sections du résumé
BACKGROUND
Because of altered airway microbiome in asthma, we analysed the bacterial species in sputum of patients with severe asthma.
METHODS
Whole genome sequencing was performed on induced sputum from non-smoking (SAn) and current or ex-smoker (SAs/ex) severe asthma patients, mild/moderate asthma (MMA) and healthy controls (HC). Data were analysed by asthma severity, inflammatory status and transcriptome-associated clusters (TACs).
RESULTS
α-diversity at the species level was lower in SAn and SAs/ex, with an increase in Haemophilus influenzae and Moraxella catarrhalis, and Haemophilus influenzae and Tropheryma whipplei, respectively, compared to HC. In neutrophilic asthma, there was greater abundance of Haemophilus influenzae and Moraxella catarrhalis and in eosinophilic asthma, Tropheryma whipplei was increased. There was a reduction in α-diversity in TAC1 and TAC2 that expressed high levels of Haemophilus influenzae and Tropheryma whipplei, and Haemophilus influenzae and Moraxella catarrhalis, respectively, compared to HC. Sputum neutrophils correlated positively with Moraxella catarrhalis and negatively with Prevotella, Neisseria and Veillonella species and Haemophilus parainfluenzae. Sputum eosinophils correlated positively with Tropheryma whipplei which correlated with pack-years of smoking. α- and β-diversities were stable at one year.
CONCLUSIONS
Haemophilus influenzae and Moraxella catarrhalis were more abundant in severe neutrophilic asthma and TAC2 linked to inflammasome and neutrophil activation, while Haemophilus influenzae and Tropheryma whipplei were highest in SAs/ex and in TAC1 associated with highest expression of IL-13 type 2 and ILC2 signatures with the abundance of Tropheryma whipplei correlating positively with sputum eosinophils. Whether these bacterial species drive the inflammatory response in asthma needs evaluation.
Substances chimiques
Inflammasomes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2906-2920Subventions
Organisme : Innovative Medicines Initiative
ID : 115010
Investigateurs
L I Andersson
(LI)
C Auffray
(C)
Y E Badi
(YE)
P Bakke
(P)
A T Bansal
(AT)
E H Bel
(EH)
J Bigler
(J)
B Billing
(B)
H Bisgaard
(H)
M J Boedigheimer
(MJ)
K Bønnelykke
(K)
J Brandsma
(J)
P Brinkman
(P)
E Bucchioni
(E)
D Burg
(D)
A Bush
(A)
M Caruso
(M)
R Chalekis
(R)
P Chanez
(P)
T Checa
(T)
C H Compton
(CH)
J Corfield
(J)
D Cunoosamy
(D)
B Dahlén
(B)
S E Dahlén
(SE)
B De Meulder
(B)
V J Erpenbeck
(VJ)
D Erzen
(D)
K Fichtner
(K)
L J Fleming
(LJ)
E Formaggio
(E)
S J Fowler
(SJ)
U Frey
(U)
M Gahlemann
(M)
T Geiser
(T)
V Goss
(V)
Y Guo
(Y)
S Hashimoto
(S)
J Haughney
(J)
G Hedlin
(G)
P W Hekking
(PW)
T Higenbottam
(T)
J M Hohlfeld
(JM)
C Holweg
(C)
I Horváth
(I)
A J James
(AJ)
R G Knowles
(RG)
J Kolmert
(J)
J Konradsen
(J)
N Krug
(N)
N Lazarinis
(N)
C-X Li
(CX)
M J Loza
(MJ)
R Lutter
(R)
A Manta
(A)
S Masefield
(S)
J G Matthews
(JG)
A Mazein
(A)
Rjm Middelveld
(R)
M Miralpeix
(M)
C S Murray
(CS)
J Musial
(J)
S Mumby
(S)
D Myles
(D)
B Nordlund
(B)
I Pandis
(I)
S Pavlidis
(S)
A Postle
(A)
P Powel
(P)
G Praticò
(G)
M Puig Valls
(M)
N Rao
(N)
S Reinke
(S)
A Roberts
(A)
G Roberts
(G)
A Rowe
(A)
T Sandström
(T)
Jpr Schofield
(J)
W Seibold
(W)
D E Shaw
(DE)
R Sigmund
(R)
F Singer
(F)
P J Skipp
(PJ)
M Smicker
(M)
A R Sousa
(AR)
M Sparreman-Mikus
(M)
M Ström
(M)
K Sun
(K)
B Thornton
(B)
M Uddin
(M)
J Vestbo
(J)
N H Vissing
(NH)
S S Wagers
(SS)
A Wheelock
(A)
C E Wheelock
(CE)
S J Wilson
(SJ)
V Yasinska
(V)
Informations de copyright
© 2023 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.
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