Why is Babesia not killed by artemisinin like Plasmodium?
Artemisinin
Babesia
Haem synthesis
Pentose phosphate pathway
Plasmodium
Single-cell sequencing
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
08 Jun 2023
08 Jun 2023
Historique:
received:
11
01
2023
accepted:
21
04
2023
medline:
12
6
2023
pubmed:
9
6
2023
entrez:
8
6
2023
Statut:
epublish
Résumé
Babesia spp. are intraerythrocytic apicomplexans that digest and utilize red blood cells in a similar way to intraerythrocytic Plasmodium spp., but unlike the latter, are not sensitive to artemisinin. A comparison of Babesia and Plasmodium genomes revealed that Babesia genomes, which are smaller than those of Plasmodium, lack numerous genes, and especially haem synthesis-related genes, that are found in the latter. Single-cell sequencing analysis showed that the different treatment groups of Babesia microti with expressed pentose phosphate pathway-related, DNA replication-related, antioxidation-related, glycolysis-related, and glutathione-related genes were not as sensitive to artemether as Plasmodium yoelii 17XNL. In particular, pentose phosphate pathway-related, DNA replication-related, and glutathione-related genes, which were actively expressed in P. yoelii 17XNL, were not actively expressed in B. microti. Supplying iron in vivo can promote the reproduction of B. microti. These results suggest that Babesia spp. lack a similar mechanism to that of malaria parasites through which the haem or iron in hemoglobin is utilized, and that this likely leads to their insensitivity to artemisinin.
Identifiants
pubmed: 37291657
doi: 10.1186/s13071-023-05783-4
pii: 10.1186/s13071-023-05783-4
pmc: PMC10249562
doi:
Substances chimiques
artemisinin
9RMU91N5K2
Artemisinins
0
Iron
E1UOL152H7
Heme
42VZT0U6YR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
193Informations de copyright
© 2023. The Author(s).
Références
Front Cell Infect Microbiol. 2017 Sep 29;7:429
pubmed: 29034218
Blood. 1994 Aug 1;84(3):910-5
pubmed: 8043872
Int J Parasitol. 2017 Mar;47(4):171-183
pubmed: 28012717
Nucleic Acids Res. 2023 Jan 6;51(D1):D587-D592
pubmed: 36300620
Antimicrob Agents Chemother. 2012 Jan;56(1):302-14
pubmed: 22083467
Sci Rep. 2016 Oct 03;6:34463
pubmed: 27694940
PLoS One. 2013 Sep 04;8(9):e72657
pubmed: 24023759
Front Microbiol. 2021 Sep 03;12:697669
pubmed: 34539601
PLoS One. 2012;7(5):e37171
pubmed: 22629364
Acta Haematol. 1996;95(1):70-7
pubmed: 8604590
Nat Biotechnol. 2015 May;33(5):495-502
pubmed: 25867923
Trans R Soc Trop Med Hyg. 2000 Sep-Oct;94(5):537-44
pubmed: 11132385
Nature. 2018 Aug;560(7719):494-498
pubmed: 30089906
J Biol Chem. 1995 Oct 20;270(42):24876-83
pubmed: 7559611
Nat Biotechnol. 2020 Dec;38(12):1408-1414
pubmed: 32747759
Sci Rep. 2016 Oct 18;6:35284
pubmed: 27752055
Parasitol Res. 2013 Aug;112(8):2983-90
pubmed: 23733233
Genome Biol. 2019 Dec 23;20(1):296
pubmed: 31870423
Parasitol Int. 2015 Apr;64(2):190-3
pubmed: 25523292
Nature. 2002 Oct 3;419(6906):498-511
pubmed: 12368864
Blood. 1988 Jul;72(1):358-61
pubmed: 3291984
Pharmacol Res. 2017 Mar;117:192-217
pubmed: 27867026
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2068-E2076
pubmed: 28242687
Malar J. 2015 Sep 30;14:382
pubmed: 26424148
Microorganisms. 2022 Aug 15;10(8):
pubmed: 36014069
Parasitol Int. 2010 Sep;59(3):481-6
pubmed: 20541037
Vet Parasitol. 2008 Oct 20;157(1-2):1-8
pubmed: 18771856
N Engl J Med. 2012 Jun 21;366(25):2397-407
pubmed: 22716978
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(1):16-20
pubmed: 12563809
N Engl J Med. 1992 Nov 19;327(21):1473-7
pubmed: 1406879
Wellcome Open Res. 2019 Mar 29;4:58
pubmed: 31080894
Nucleic Acids Res. 2022 Jan 7;50(D1):D898-D911
pubmed: 34718728
Science. 2017 Jul 28;357(6349):409-413
pubmed: 28596308
FEBS J. 2021 Jan;288(2):382-404
pubmed: 32530125
Lancet. 2005 Aug 27-Sep 2;366(9487):717-25
pubmed: 16125588
Parasit Vectors. 2020 Jul 20;13(1):362
pubmed: 32690081