Development and validation of a real-time PCR test to detect Bartonella quintana in clinical samples.

Humans Bartonella quintana / genetics Real-Time Polymerase Chain Reaction Bartonella henselae
Clinical diagnostics Laboratory method Real-time PCR

Journal

Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899

Informations de publication

Date de publication:
Aug 2023
Historique:
received: 13 01 2023
revised: 19 05 2023
accepted: 25 05 2023
medline: 3 7 2023
pubmed: 10 6 2023
entrez: 9 6 2023
Statut: ppublish

Résumé

This study reports on the validation of a real-time polymerase chain reaction test targeting the vomp region of Bartonella quintana. The assay displayed 100% sensitivity and specificity for the 52 bloods and 159 cultures tested. Molecular diagnosis of Bartonella quintana can aid clinical treatment during acute infection.

Identifiants

DOI: 10.1016/j.diagmicrobio.2023.116000 PMID: 37295184
pubmed: 37295184
pii: S0732-8893(23)00110-4
doi: 10.1016/j.diagmicrobio.2023.116000
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

116000

Informations de copyright

Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors report no conflicts of interest relevant to this article.

Auteurs

Jamie Choat (J)

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.

Brook Yockey (B)

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.

Sarah W Sheldon (SW)

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.

Ryan Pappert (R)

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.

Jeannine Petersen (J)

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.

Elizabeth A Dietrich (EA)

Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA. Electronic address: wul2@cdc.gov.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Development and validation of a real-time PCR...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Bartonellaceae › Bartonella › Bartonella quintana › Development and validation of a real-time PCR...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Development and validation of a real-time PCR...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Bartonellaceae › Bartonella › Bartonella henselae › Development and validation of a real-time PCR...