Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts' response to fibrotic growth factors.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 06 2023
09 06 2023
Historique:
received:
20
02
2023
accepted:
03
06
2023
medline:
12
6
2023
pubmed:
10
6
2023
entrez:
9
6
2023
Statut:
epublish
Résumé
Systemic Sclerosis (SSc) hallmark is skin fibrosis, but up to 80% of the patients have fibrotic involvement in the pulmonary system. Antifibrotic drugs which have failed in a general SSc population have now been approved in patients with SSc-associated interstitial lung disease (ILD). This indicates that the fibrotic progression and regulation of fibroblasts likely depend on local factors specific to the tissue type. This study investigated the difference between dermal and pulmonary fibroblasts in a fibrotic setting, mimicking the extracellular matrix. Primary healthy fibroblasts were grown in a crowded environment and stimulated with TGF-β1 and PDGF-AB. The viability, morphology, migration capacity, extracellular matrix formation, and gene expression were assessed: TGF-β1 only increased the viability in the dermal fibroblasts. PDGF-AB increased the migration capacity of dermal fibroblasts while the pulmonary fibroblasts fully migrated. The morphology of the fibroblasts was different without stimulation. TGF-β1 increased the formation of type III collagen in pulmonary fibroblasts, while PDGF-AB increased it in dermal fibroblasts. The gene expression trend of type VI collagen was the opposite after PDGF-AB stimulation. The fibroblasts exhibit different response profiles to TGF-β1 and PDGF-AB; this suggests that drivers of fibrosis are tissue-dependent, which needs to be considered in drug development.
Identifiants
pubmed: 37296166
doi: 10.1038/s41598-023-36416-6
pii: 10.1038/s41598-023-36416-6
pmc: PMC10256773
doi:
Substances chimiques
Transforming Growth Factor beta1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9411Informations de copyright
© 2023. The Author(s).
Références
PLoS One. 2018 Dec 3;13(12):e0207324
pubmed: 30507931
Am J Physiol Lung Cell Mol Physiol. 2017 Jun 1;312(6):L896-L902
pubmed: 28314802
Sci Rep. 2020 Oct 14;10(1):17300
pubmed: 33057073
Biomarkers. 2011 Nov;16(7):616-28
pubmed: 21988680
BMC Pulm Med. 2018 Apr 27;18(1):63
pubmed: 29703175
Am J Transl Res. 2013 Apr 19;5(3):303-15
pubmed: 23634241
BMB Rep. 2021 Jun;54(6):329-334
pubmed: 34078526
Sci Rep. 2021 Mar 23;11(1):6655
pubmed: 33758206
Front Immunol. 2018 Aug 13;9:1865
pubmed: 30150989
Thorax. 1995 Sep;50(9):984-9
pubmed: 8539681
Mod Rheumatol. 2020 Mar;30(2):225-231
pubmed: 31747840
J Clin Med. 2022 Aug 08;11(15):
pubmed: 35956246
BMC Pulm Med. 2021 Nov 23;21(1):382
pubmed: 34814865
Fibrogenesis Tissue Repair. 2013 May 10;6(1):10
pubmed: 23663505
Biochem Biophys Res Commun. 2016 Sep 16;478(2):586-91
pubmed: 27475500
Br J Pharmacol. 2009 Nov;158(5):1196-209
pubmed: 19785660
Cell Signal. 2016 Apr;28(4):307-15
pubmed: 26795953
Curr Opin Rheumatol. 2018 Nov;30(6):588-593
pubmed: 30148801
Rheumatology (Oxford). 2010 Feb;49(2):259-63
pubmed: 20007285
Eur J Rheumatol. 2020 Oct;7(Suppl 3):S242-S248
pubmed: 32697934
Respirology. 2021 Jun;26(6):582-589
pubmed: 33834579
Front Pharmacol. 2022 Jan 31;13:823085
pubmed: 35173620
Development. 2003 Oct;130(20):4769-84
pubmed: 12952899
Nat Rev Dis Primers. 2015 Apr 23;1:15002
pubmed: 27189141
Sci Signal. 2019 Feb 26;12(570):
pubmed: 30808818
Lancet. 2017 Oct 7;390(10103):1685-1699
pubmed: 28413064
Respir Res. 2020 Jan 15;21(1):25
pubmed: 31941499
Nat Rev Genet. 2012 Jul 18;13(8):552-64
pubmed: 22805708
Cell Physiol Biochem. 2019;52(4):822-837
pubmed: 30946557
Assay Drug Dev Technol. 2014 May;12(4):207-18
pubmed: 24831787
Am J Pathol. 2007 Jun;170(6):1807-16
pubmed: 17525249
Physiol Rev. 1999 Oct;79(4):1283-316
pubmed: 10508235
J Scleroderma Relat Disord. 2017 May-Aug;2(2):69-134
pubmed: 29270465
N Engl J Med. 2014 May 29;370(22):2071-82
pubmed: 24836310
Adv Drug Deliv Rev. 2017 Nov 1;121:43-56
pubmed: 28736303
Nat Rev Rheumatol. 2019 Dec;15(12):705-730
pubmed: 31712723
Curr Protoc Cell Biol. 2016 Jun 01;71:10.9.1-10.9.34
pubmed: 27245425
Respir Res. 2021 Jul 15;22(1):205
pubmed: 34261485
Ann Rheum Dis. 2018 Sep;77(9):1362-1371
pubmed: 29853453
N Engl J Med. 2019 Jun 27;380(26):2518-2528
pubmed: 31112379
Lancet Respir Med. 2015 Jun;3(6):462-72
pubmed: 25770676
Adv Drug Deliv Rev. 2018 Apr;129:78-94
pubmed: 29269274
Front Pharmacol. 2014 May 27;5:123
pubmed: 24904424
Am J Respir Cell Mol Biol. 2001 May;24(5):591-8
pubmed: 11350829
Ann Rheum Dis. 2016 May;75(5):883-90
pubmed: 25858641
J Neuroimmune Pharmacol. 2014 Mar;9(2):69-79
pubmed: 23793451
J Cell Sci. 2010 Dec 15;123(Pt 24):4195-200
pubmed: 21123617
Sci Rep. 2015 Mar 04;5:8729
pubmed: 25736020
J Clin Invest. 2007 Mar;117(3):524-9
pubmed: 17332879
Am J Respir Crit Care Med. 1995 Jun;151(6):1956-64
pubmed: 7767545
PLoS One. 2015 Dec 07;10(12):e0144525
pubmed: 26641456
Res Exp Med (Berl). 1993;193(4):195-205
pubmed: 8235072
Clin Biochem. 2010 Oct;43(15):1249-56
pubmed: 20709044
Respir Res. 2019 Jul 12;20(1):148
pubmed: 31299951
Ann Rheum Dis. 2007 Jul;66(7):940-4
pubmed: 17329309
Biochem Biophys Res Commun. 1995 Apr 17;209(2):393-9
pubmed: 7733905
Respir Res. 2020 May 7;21(1):108
pubmed: 32381012
Cancers (Basel). 2022 Feb 06;14(3):
pubmed: 35159087
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Biomarkers. 2019 Jun;24(4):373-378
pubmed: 30821519
Arthritis Rheum. 2007 Mar 15;57(2):318-26
pubmed: 17330281
Annu Rev Biophys. 2008;37:375-97
pubmed: 18573087