The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells.
Chemically defined differentiation
Hematopoietic precursor cells
Induced pluripotent stem (iPS) cells
Microglia (iMG)
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
12
6
2023
pubmed:
10
6
2023
entrez:
10
6
2023
Statut:
ppublish
Résumé
The following protocol describes the generation of microglia cells from human-induced pluripotent stem cells (hiPSCs) using commercially available kits by StemCell Technologies. This protocol consists of three major steps: (1) Differentiation of hematopoietic precursor cells, (2) Microglia differentiation, and (3) Microglia maturation. Assays are described to characterize hematopoietic precursor cells and mature microglia.
Identifiants
pubmed: 37300767
doi: 10.1007/978-1-0716-3287-1_6
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-78Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM119943
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG077697
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Wurm J, Konttinen H, Andressen C et al (2021) Microglia development and maturation and its implications for induction of microglia-like cells from human iPSCs. Int J Mol Sci 22:3088
doi: 10.3390/ijms22063088
pubmed: 33803024
pmcid: 8002593
Abud E, Ramirez R, Martinez E et al (2017) iPSC-derived human microglia-like cells to study neurological diseases. Neuron 94(2):278–293
doi: 10.1016/j.neuron.2017.03.042
pubmed: 28426964
pmcid: 5482419
Guttikonda S, Sikkema L, Tchieu J et al (2021) Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in Alzheimer’s disease. Nat Neurosci 24:343–354
doi: 10.1038/s41593-020-00796-z
pubmed: 33558694
pmcid: 8382543