Protocol to generate an in vitro model to study vascular calcification using human endothelial and smooth muscle cells.
Biotechnology and bioengineering
Cell Biology
Tissue Engineering
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
09 Jun 2023
09 Jun 2023
Historique:
received:
22
12
2022
revised:
17
02
2023
accepted:
29
04
2023
medline:
10
6
2023
pubmed:
10
6
2023
entrez:
10
6
2023
Statut:
aheadofprint
Résumé
Vascular calcification is a systemic disease characterized by calcium salt deposition within vascular walls. Here, we present a protocol for establishing an advanced dynamic in vitro co-culture system using endothelial and smooth muscle cells to replicate vascular tissue complexity. We describe steps for cell culture and seeding in a double-flow bioreactor that recreates the action of blood in humans. We then detail the induction of calcification, setting up of the bioreactor, followed by cell viability assessment and calcium quantification.
Identifiants
pubmed: 37300824
pii: S2666-1667(23)00295-2
doi: 10.1016/j.xpro.2023.102328
pmc: PMC10323150
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102328Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Kidney Int. 2004 Dec;66(6):2293-9
pubmed: 15569318
Biochem Biophys Res Commun. 2020 Sep 17;530(2):462-470
pubmed: 32560961