Alaska Backcountry Expeditionary Hunting Promotes Sustained Muscle Protein Synthesis.


Journal

Wilderness & environmental medicine
ISSN: 1545-1534
Titre abrégé: Wilderness Environ Med
Pays: United States
ID NLM: 9505185

Informations de publication

Date de publication:
Sep 2023
Historique:
received: 12 02 2023
revised: 02 05 2023
accepted: 05 05 2023
pmc-release: 01 09 2024
medline: 28 8 2023
pubmed: 11 6 2023
entrez: 10 6 2023
Statut: ppublish

Résumé

We have previously described negative energy balance (ie, -9.7±3.4 MJ/d) and weight loss (Δ-1.5 ± 0.7 kg) influenced by high levels of energy expenditure (ie, 17.4±2.6 MJ/d) during remote expeditionary hunting in Alaska. Despite negative energy balance, participants retained skeletal muscle. The purpose of this pilot study was to measure skeletal muscle protein synthesis and examine molecular markers of skeletal muscle protein metabolism under similar conditions of physical and nutrient stress. The "virtual biopsy method" was used to evaluate integrated fractional synthetic rates (FSRs) of muscle protein from blood samples in 4 participants. Muscle biopsies were taken to measure molecular markers of muscle protein kinetics (ie, FSTL1, MEF2, MYOD1, B2M, and miR-1-3p, -206, -208b, 23a, and 499a) using real-time polymerase chain reaction. Our findings in 4 participants (2 females [28 and 62 y of age; 66.2 and 71.8 kg body weight; 25.5 and 26.7 kg/m Preservation of skeletal muscle under conditions of physical and nutrient stress seems to be supported by positive inflection of skeletal muscle FSR and molecular activation.

Identifiants

pubmed: 37301628
pii: S1080-6032(23)00093-5
doi: 10.1016/j.wem.2023.05.003
pmc: PMC10526753
mid: NIHMS1907656
pii:
doi:

Substances chimiques

Muscle Proteins 0
FSTL1 protein, human 158709-61-6
Follistatin-Related Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

341-345

Subventions

Organisme : NIGMS NIH HHS
ID : P20 GM103395
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM130443
Pays : United States

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Références

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Auteurs

Robert H Coker (RH)

Montana Center for Work Physiology and Exercise Metabolism, University of Montana, Missoula, MT. Electronic address: robert.coker@umontana.edu.

Brent C Ruby (BC)

Montana Center for Work Physiology and Exercise Metabolism, University of Montana, Missoula, MT.

Melynda S Coker (MS)

Montana Center for Work Physiology and Exercise Metabolism, University of Montana, Missoula, MT.

Larry Bartlett (L)

Pristine Ventures, Inc, Fairbanks, AK.

Brandon Kowalski (B)

Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK.

Anna V Goropashnaya (AV)

Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK.

Terry Bateman (T)

Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK.

Mahalakshmi Shankaran (M)

Department of Nutritional Sciences and Toxicology, University of California Berkeley, Berkeley, CA.

Marc Hellerstein (M)

Department of Nutritional Sciences and Toxicology, University of California Berkeley, Berkeley, CA.

William J Evans (WJ)

Department of Nutritional Sciences and Toxicology, University of California Berkeley, Berkeley, CA.

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Classifications MeSH