A human septin octamer complex sensitive to membrane curvature drives membrane deformation with a specific mesh-like organization.
Curvature
Cytoskeleton
Membrane
Septin
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
01 06 2023
01 06 2023
Historique:
received:
16
11
2022
accepted:
04
05
2023
medline:
13
6
2023
pubmed:
12
6
2023
entrez:
12
6
2023
Statut:
ppublish
Résumé
Septins are cytoskeletal proteins interacting with the inner plasma membrane and other cytoskeletal partners. Being key in membrane remodeling processes, they often localize at specific micrometric curvatures. To analyze the behavior of human septins at the membrane and decouple their role from other partners, we used a combination of bottom-up in vitro methods. We assayed their ultrastructural organization, their curvature sensitivity, as well as their role in membrane reshaping. On membranes, human septins organize into a two-layered mesh of orthogonal filaments, instead of generating parallel sheets of filaments observed for budding yeast septins. This peculiar mesh organization is sensitive to micrometric curvature and drives membrane reshaping as well. The observed membrane deformations together with the filamentous organization are recapitulated in a coarse-grained computed simulation to understand their mechanisms. Our results highlight the specific organization and behavior of animal septins at the membrane as opposed to those of fungal proteins.
Identifiants
pubmed: 37305997
pii: 316658
doi: 10.1242/jcs.260813
pmc: PMC10281262
pii:
doi:
Substances chimiques
Septins
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
Références
Nat Rev Mol Cell Biol. 2012 Feb 08;13(3):183-94
pubmed: 22314400
Soft Matter. 2021 Sep 14;17(34):7953-7962
pubmed: 34378621
J Cell Biol. 2019 Apr 1;218(4):1128-1137
pubmed: 30659102
Nat Cell Biol. 2014 Apr;16(4):322-34
pubmed: 24633326
J Cell Sci. 2021 Aug 1;134(15):
pubmed: 34350965
IUCrJ. 2020 Mar 28;7(Pt 3):462-479
pubmed: 32431830
J Mol Biol. 2021 Apr 30;433(9):166889
pubmed: 33639214
J Pathol. 2004 Nov;204(4):489-505
pubmed: 15495264
PLoS One. 2014 Dec 10;9(12):e113916
pubmed: 25494357
J Cell Biol. 2011 Dec 12;195(6):993-1004
pubmed: 22144691
PLoS Genet. 2017 Mar 27;13(3):e1006631
pubmed: 28346465
Phys Rev E. 2019 Feb;99(2-1):022414
pubmed: 30934309
Mol Biol Cell. 2002 Oct;13(10):3532-45
pubmed: 12388755
Front Cell Dev Biol. 2022 Jan 17;9:824850
pubmed: 35111763
J Mol Biol. 2021 Jul 23;433(15):167096
pubmed: 34116125
EMBO J. 2009 Nov 18;28(22):3476-84
pubmed: 19779463
Curr Biol. 2019 Jul 8;29(13):2174-2182.e7
pubmed: 31204162
J Membr Biol. 2022 Dec;255(6):757-777
pubmed: 36197492
Curr Biol. 1999 Dec 16-30;9(24):1458-67
pubmed: 10607590
Front Cell Dev Biol. 2017 Apr 11;5:35
pubmed: 28443280
J Cell Sci. 2018 Jan 11;131(1):
pubmed: 29326311
J Mol Biol. 2010 Dec 10;404(4):711-31
pubmed: 20951708
J Cell Biol. 2023 Mar 6;222(3):
pubmed: 36562751
FEBS Lett. 2007 Nov 27;581(28):5526-32
pubmed: 17967425
Nanoscale. 2017 Feb 2;9(5):2030-2037
pubmed: 28106209
Cell Biochem Biophys. 2012 Mar;62(2):317-28
pubmed: 22001952
Cell Mol Life Sci. 2009 Oct;66(20):3309-23
pubmed: 19597764
Dev Cell. 2005 Mar;8(3):343-52
pubmed: 15737930
J Cell Biol. 2012 Jan 9;196(1):103-14
pubmed: 22232702
Elife. 2016 Aug 09;5:
pubmed: 27504968
Z Naturforsch C. 1973 Nov-Dec;28(11):693-703
pubmed: 4273690
Mol Biol Cell. 2012 Feb;23(3):423-32
pubmed: 22160597
Trends Biochem Sci. 2012 Dec;37(12):526-33
pubmed: 23058040
Curr Biol. 2009 Jan 27;19(2):140-5
pubmed: 19167227
Dev Cell. 2009 Apr;16(4):493-506
pubmed: 19386259
Cytoskeleton (Hoboken). 2019 Jan;76(1):92-103
pubmed: 30070077
Soft Matter. 2021 Jan 22;17(2):276-287
pubmed: 32406895
J Biochem. 2003 Oct;134(4):491-6
pubmed: 14607974
Trends Biochem Sci. 2017 Dec;42(12):961-976
pubmed: 29089160
Soft Matter. 2020 Jan 22;16(3):595-603
pubmed: 31776531
Soft Matter. 2015 Apr 21;11(15):3067-75
pubmed: 25742777
Curr Opin Microbiol. 2006 Dec;9(6):559-65
pubmed: 17067846
Curr Biol. 2007 Oct 23;17(20):1752-8
pubmed: 17935993
Biophys J. 2013 Mar 5;104(5):1018-28
pubmed: 23473484
Elife. 2021 Apr 13;10:
pubmed: 33847563
Proc Natl Acad Sci U S A. 2008 Jun 17;105(24):8274-9
pubmed: 18550837
Nat Commun. 2019 Jan 24;10(1):420
pubmed: 30679428
Curr Opin Cell Biol. 2018 Feb;50:27-34
pubmed: 29438904
Science. 2010 Jul 23;329(5990):436-9
pubmed: 20558667
Microb Cell. 2019 May 22;6(6):295-298
pubmed: 31172014