Clostridium perfringens epsilon-toxin requires acid sphingomyelinase for cellular entry.

Animals Dogs Sphingomyelin Phosphodiesterase / genetics Bacterial Toxins / genetics Madin Darby Canine Kidney Cells Ceramides / metabolism Clostridium perfringens / metabolism
Acid sphingomyelinase C. perfringens epsilon-Toxin Internalization

Journal

Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216

Informations de publication

Date de publication:
Aug 2023
Historique:
received: 18 04 2023
revised: 08 06 2023
accepted: 09 06 2023
medline: 4 10 2023
pubmed: 13 6 2023
entrez: 12 6 2023
Statut: ppublish

Résumé

Clostridium perfringens epsilon-toxin is considered to be a crucial agent in enterotoxemia in domestic animals. Epsilon-toxin enters host cells via endocytosis and results in the formation of late endosome/lysosome-derived vacuoles. In the present study, we found that acid sphingomyelinase promotes the internalization of epsilon-toxin in MDCK cells. We measured the extracellular release of acid sphingomyelinase (ASMase) by epsilon-toxin. We examined the role of ASMase in epsilon-toxin-induced cytotoxicity using selective inhibitors and knockdown of ASMase. Production of ceramide after toxin treatment was determined by immunofluorescence technique. Blocking agents of ASMase and exocytosis of lysosomes inhibited this epsilon-toxin-induced vacuole formation. Lysosomal ASMase was liberated to extracellular space during treatment of the cells with epsilon-toxin in the presence of Ca Based on the present results, ASMase is required for efficient internalization of epsilon-toxin.

Identifiants

DOI: 10.1016/j.anaerobe.2023.102753 PMID: 37308057
pubmed: 37308057
pii: S1075-9964(23)00062-8
doi: 10.1016/j.anaerobe.2023.102753
pii:
doi:

Substances chimiques

Clostridium perfringens epsilon-toxin 0
Sphingomyelin Phosphodiesterase EC 3.1.4.12
Bacterial Toxins 0
Ceramides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102753

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that there are no conflicts of interest.

Auteurs

Yoshihiko Sakaguchi (Y)

Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 180, Tokushima, 770-8514, Japan.

Keiko Kobayashi (K)

Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 180, Tokushima, 770-8514, Japan.

Masaya Takehara (M)

Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 180, Tokushima, 770-8514, Japan.

Masahiro Nagahama (M)

Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 180, Tokushima, 770-8514, Japan. Electronic address: nagahama@ph.bunri-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Clostridium perfringens epsilon-toxin requires...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Carnivora › Pinnipedia › Canidae › Chiens › Clostridium perfringens epsilon-toxin requires...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Phosphodiesterases › Sphingomyeline phosphodiesterase › Clostridium perfringens epsilon-toxin requires...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines bactériennes › Clostridium perfringens epsilon-toxin requires...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules rénales canines Madin-Darby › Clostridium perfringens epsilon-toxin requires...
questionsmedicales.fr › Lipides › Lipides membranaires › Sphingolipides › Glycosphingolipides › Glycosphingolipides neutres › Céramides › Clostridium perfringens epsilon-toxin requires...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Clostridium perfringens › Clostridium perfringens epsilon-toxin requires...