Genome-wide genetic variation and molecular surveillance of drug resistance in Plasmodium falciparum isolates from asymptomatic individuals in Ouélessébougou, Mali.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 06 2023
12 06 2023
Historique:
received:
06
03
2023
accepted:
27
05
2023
medline:
14
6
2023
pubmed:
13
6
2023
entrez:
12
6
2023
Statut:
epublish
Résumé
Sequence analysis of Plasmodium falciparum parasites is informative in ensuring sustained success of malaria control programmes. Whole-genome sequencing technologies provide insights into the epidemiology and genome-wide variation of P. falciparum populations and can characterise geographical as well as temporal changes. This is particularly important to monitor the emergence and spread of drug resistant P. falciparum parasites which is threatening malaria control programmes world-wide. Here, we provide a detailed characterisation of genome-wide genetic variation and drug resistance profiles in asymptomatic individuals in South-Western Mali, where malaria transmission is intense and seasonal, and case numbers have recently increased. Samples collected from Ouélessébougou, Mali (2019-2020; n = 87) were sequenced and placed in the context of older Malian (2007-2017; n = 876) and African-wide (n = 711) P. falciparum isolates. Our analysis revealed high multiclonality and low relatedness between isolates, in addition to increased frequencies of molecular markers for sulfadoxine-pyrimethamine and lumefantrine resistance, compared to older Malian isolates. Furthermore, 21 genes under selective pressure were identified, including a transmission-blocking vaccine candidate (pfCelTOS) and an erythrocyte invasion locus (pfdblmsp2). Overall, our work provides the most recent assessment of P. falciparum genetic diversity in Mali, a country with the second highest burden of malaria in West Africa, thereby informing malaria control activities.
Identifiants
pubmed: 37308503
doi: 10.1038/s41598-023-36002-w
pii: 10.1038/s41598-023-36002-w
pmc: PMC10258750
doi:
Substances chimiques
Antiparasitic Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9522Subventions
Organisme : Bill & Melinda Gates Foundation
ID : INV-002098
Pays : United States
Organisme : Medical Research Council
ID : MR/M01360X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N010469/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R020973/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/X005895/1
Pays : United Kingdom
Informations de copyright
© 2023. The Author(s).
Références
Nat Commun. 2016 May 18;7:11553
pubmed: 27189525
Clin Infect Dis. 2018 Jun 1;66(12):1883-1891
pubmed: 29304258
Open Forum Infect Dis. 2016 Oct 25;4(1):ofw229
pubmed: 28480232
Wellcome Open Res. 2023 Jan 16;8:22
pubmed: 36864926
Mol Ecol Resour. 2016 Mar;16(2):540-8
pubmed: 26417651
Malar J. 2021 Mar 4;20(1):129
pubmed: 33663492
Sci Rep. 2021 Oct 6;11(1):19809
pubmed: 34615917
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
Malar J. 2018 Nov 7;17(1):413
pubmed: 30404640
J Infect Public Health. 2022 May;15(5):550-557
pubmed: 35447389
Malar J. 2016 Dec 20;15(1):597
pubmed: 27998271
Int J Environ Res Public Health. 2020 Jun 04;17(11):
pubmed: 32512740
Elife. 2021 Jul 19;10:
pubmed: 34279219
Malar J. 2018 Oct 5;17(1):352
pubmed: 30290815
J Infect Dis. 2014 Dec 15;210(12):1991-2000
pubmed: 24948693
Bioinformatics. 2017 Jul 15;33(14):2071-2077
pubmed: 28334194
Nat Med. 2020 Oct;26(10):1602-1608
pubmed: 32747827
Malar J. 2021 Nov 25;20(1):447
pubmed: 34823528
Malar J. 2019 Nov 12;18(1):361
pubmed: 31718631
Front Immunol. 2013 Jul 04;4:176
pubmed: 23847617
Mol Ecol Resour. 2017 Jan;17(1):78-90
pubmed: 27863062
BMJ Glob Health. 2017 May 16;2(2):e000176
pubmed: 29242750
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1124-9
pubmed: 9037017
Front Genet. 2022 Oct 04;13:943445
pubmed: 36267403
Malar J. 2011 May 14;10:123
pubmed: 21569596
J Infect Dis. 2002 Feb 1;185(3):380-8
pubmed: 11807721
J Infect Dis. 2003 Jun 15;187(12):1870-5
pubmed: 12792863
Malar J. 2022 Feb 8;21(1):39
pubmed: 35135546
Bioinformatics. 2007 Jan 1;23(1):127-8
pubmed: 17050570
Proc Natl Acad Sci U S A. 1988 Dec;85(23):9109-13
pubmed: 3057499
Bioinformatics. 2017 Jul 01;33(13):2037-2039
pubmed: 28205675
Vaccine. 2011 Aug 11;29(35):5940-9
pubmed: 21722682
J Infect Dis. 2013 Mar 1;207(5):842-7
pubmed: 23225895
Lancet Microbe. 2022 Jan;3(1):e41-e51
pubmed: 35028628
Trop Med Int Health. 2004 May;9(5):624-9
pubmed: 15117308
Expert Rev Anti Infect Ther. 2017 Jun;15(6):527-543
pubmed: 28355493
Nat Commun. 2017 Oct 26;8(1):1133
pubmed: 29074880
Lancet Microbe. 2022 May;3(5):e336-e347
pubmed: 35544095
Nat Rev Microbiol. 2014 Dec;12(12):833-40
pubmed: 25329408
J Infect Dis. 2014 Oct 1;210(7):1110-4
pubmed: 24723474
PLoS One. 2016 Mar 31;11(3):e0151565
pubmed: 27031231
Malar J. 2018 May 15;17(1):196
pubmed: 29764422
J Infect Dis. 2005 Mar 15;191(6):1014-7
pubmed: 15717281
N Engl J Med. 2021 Sep 23;385(13):1163-1171
pubmed: 34551228
Malar J. 2016 Sep 05;15:452
pubmed: 27596849
Nat Rev Genet. 2021 Aug;22(8):502-517
pubmed: 33833443
Curr Opin Infect Dis. 2021 Oct 1;34(5):432-439
pubmed: 34267045
Malar J. 2011 May 09;10:116
pubmed: 21554692
J Infect Dis. 2001 May 15;183(10):1535-8
pubmed: 11319692