Differential expression of the angiotensin receptors (AT1, AT2, and AT4) in the placental bed of HIV-infected preeclamptic women of African ancestry.
Angiotensin II-type 1 receptor (AT1R)
Angiotensin II-type 2 receptor (AT2R)
Angiotensin II-type 4 receptor (AT4R)
Preeclampsia
Renin-Angiotensin Aldosterone System (RAAS)
Journal
Hypertension research : official journal of the Japanese Society of Hypertension
ISSN: 1348-4214
Titre abrégé: Hypertens Res
Pays: England
ID NLM: 9307690
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
25
11
2022
accepted:
28
04
2023
revised:
05
04
2023
medline:
8
8
2023
pubmed:
13
6
2023
entrez:
12
6
2023
Statut:
ppublish
Résumé
The Renin-Angiotensin-Aldosterone System (RAAS) is implicated in the pathophysiology of preeclampsia (PE). There is a paucity of data on uteroplacental angiotensin receptors AT1-2 and 4. We evaluated the immunoexpression of AT1R, AT2R, and AT4R within the placental bed of PE vs. normotensive (N) pregnancies stratified by HIV status. Placental bed (PB) biopsies (n = 180) were obtained from N and PE women. Both groups were stratified by HIV status and gestational age into early-and late onset-PE. Immuno-labeling of AT1R, AT2R, and AT4R was quantified using morphometric image analysis. Immunostaining of PB endothelial cells (EC) and smooth muscle cells of spiral arteries (VSMC) displayed an upregulation of AT1R expression compared to the N group (p < 0.0001). Downregulation of AT2R and AT4R expression was observed in PE vs. N group (p = 0.0042 and p < 0.0001), respectively. AT2R immunoexpression declined between HIV+ve and HIV-ve groups, while AT1R and AT4R displayed an increase. An increase in AT1R expression was noted in the EOPE-ve/+ve and LOPE-ve/+ve compared to N-ve/N+ve. In contrast, AT2R and AT4R expression decreased in EOPE-ve/+ve and LOPE-ve/+ve compared to N-ve/N+ve. We demonstrate a significant downregulation of AT2R and AT4R with a concomitant elevated AT1R immunoexpression within PB of HIV-infected PE women. In addition, a decline in AT2R and AT4R with an increase in AT1R immunoexpression in PE, EOPE, and LOPE vs. normotensive pregnancies, irrespective of HIV status. Thus highlighting differential immunoexpression of uteroplacental RAAS receptors based on pregnancy type, HIV status, and gestational age.
Identifiants
pubmed: 37308552
doi: 10.1038/s41440-023-01314-x
pii: 10.1038/s41440-023-01314-x
pmc: PMC10404513
doi:
Substances chimiques
Receptors, Angiotensin
0
eosinylphosphatidylethanolamine
0
Receptor, Angiotensin, Type 1
0
Receptor, Angiotensin, Type 2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1970-1982Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2023. The Author(s).
Références
South Africa: Statistical release P0302 Mid-year estimates. (various years) https://www.statssa.gov.za/publications/P0302/P03022022.pdf . Accessed 7 November 2022.
Woldesenbet S, Kufa T, Lombard C, Manda S, Morof D, Cheyip M, et al. The prevalence of unintended pregnancy and its association with HIV status among pregnant women in South Africa, a national antenatal survey, 2019. Sci Rep. 2021;11:1–11.
doi: 10.1038/s41598-021-03096-z
The Status of Women’s Health in South Africa: Evidence from selected indicators https://www.statssa.gov.za/?page_id=1856&PPN=03-00-18&SCH=73277 . StatsSA; 2022. Accessed 7 November 2022.
Magee LA, Nicolaides KH, Von Dadelszen P. Preeclampsia. N Engl J Med. 2022;386:1817–32.
pubmed: 35544388
doi: 10.1056/NEJMra2109523
Suvakov S, Bonner E, Nikolic V, Jerotic D, Simic TP, Garovic VD, et al. Overlapping pathogenic signalling pathways and biomarkers in preeclampsia and cardiovascular disease. Pregnancy Hypertens. 2020;20:131–6.
pubmed: 32299060
doi: 10.1016/j.preghy.2020.03.011
Ahn H, Park J, Gilman‐Sachs A, Kwak‐Kim J. Immunologic characteristics of preeclampsia, a comprehensive review. Am J Reprod Immunol. 2011;65:377–94.
pubmed: 20825381
doi: 10.1111/j.1600-0897.2010.00913.x
Liu H, Ning F, Lash GE. Contribution of vascular smooth muscle cell apoptosis to spiral artery remodeling in early human pregnancy. Placenta 2022;120:10–7.
pubmed: 35158146
doi: 10.1016/j.placenta.2022.02.005
Raymond D, Peterson E. A critical review of early-onset and late-onset preeclampsia. Obstetrical Gynecol Surv. 2011;66:497–506.
doi: 10.1097/OGX.0b013e3182331028
van der Graaf AM, Toering TJ, Faas MM, Titia Lely A. From preeclampsia to renal disease: a role of angiogenic factors and the renin–angiotensin aldosterone system? Nephrol Dialys Transplant. 2012;27:iii51–iii57.
Ma K, Gao W, Xu H, Liang W, Ma G. Role and Mechanism of the Renin-Angiotensin-Aldosterone System in the Onset and Development of Cardiorenal Syndrome. J Renin-Angiotensin-Aldosterone Syst. 2022;2022:1–8.
doi: 10.1155/2022/3239057
Balakumar P, Handa S, Alqahtani A, Alqahtani T, Khan NA, LakshmiRaj RS, et al. Unraveling the Differentially Articulated Axes of the Century-Old Renin–Angiotensin–Aldosterone System: Potential Therapeutic Implications. Cardiovasc Toxicol. 2022;22:246–53.
pubmed: 35143015
doi: 10.1007/s12012-022-09724-y
Doan TNA, Bianco-Miotto T, Parry L, Winter M. The role of Angiotensin II and relaxin in vascular adaptation to pregnancy. Reproduction 2022;164:R87–R99.
pubmed: 36018774
doi: 10.1530/REP-21-0428
Gathiram P, Moodley J. The role of the renin-angiotensin-aldosterone system in preeclampsia: a review. Curr Hypertens Rep. 2020;22:1–9.
doi: 10.1007/s11906-020-01098-2
Verdonk K, Visser W, Van Den Meiracker AH, Danser AJ. The renin–angiotensin–aldosterone system in pre-eclampsia: the delicate balance between good and bad. Clin Sci. 2014;126:537–44.
doi: 10.1042/CS20130455
Lumbers ER, Pringle KG. Roles of the circulating renin-angiotensin-aldosterone system in human pregnancy. Am J Physiol-Regul Integr Comp Physiol. 2014;306:R91–R101.
pubmed: 24089380
doi: 10.1152/ajpregu.00034.2013
Irani RA, Xia Y. The functional role of the renin–angiotensin system in pregnancy and preeclampsia. Placenta 2008;29:763–71.
pubmed: 18687466
pmcid: 2614866
doi: 10.1016/j.placenta.2008.06.011
Merrill DC, Karoly M, Chen K, Ferrario CM, Brosnihan KB. Angiotensin-(1–7) in normal and preeclamptic pregnancy. Endocrine 2002;18:239–45.
pubmed: 12450315
doi: 10.1385/ENDO:18:3:239
Brosnihan KB, Merrill DC, Yamaleyeva LM, Chen K, Neves L, Joyner J, et al. Longitudinal study of angiotensin peptides in normal and pre-eclamptic pregnancy. Endocrine 2020;69:410–9.
pubmed: 32319014
doi: 10.1007/s12020-020-02296-3
Cosarderelioglu C, Nidadavolu LS, George CJ, Marx-Rattner R, Powell L, Xue Q-L, et al. Angiotensin receptor blocker use is associated with upregulation of the memory-protective angiotensin type 4 receptor (AT4R) in the postmortem brains of individuals without cognitive impairment. GeroScience. 2023;45:371–84.
Cooper A, Robinson G, Vinson G, Cheung W, Pipkin FB. The localization and expression of the renin–angiotensin system in the human placenta throughout pregnancy. Placenta 1999;20:467–74.
pubmed: 10419812
doi: 10.1053/plac.1999.0404
Singh S, Moodley J, Khaliq OP, Naicker T. a narrative review of the renin-angiotensin-aldosterone system in the placenta and placental bed of HIV infected women of African ancestry with preeclampsia. Curr Hypertens Rep. 2021;23:39.
pubmed: 34415457
pmcid: 8377458
doi: 10.1007/s11906-021-01158-1
Seki H. The role of the renin–angiotensin system in the pathogenesis of preeclampsia–new insights into the renin–angiotensin system in preeclampsia. Med Hypotheses. 2014;82:362–7.
pubmed: 24495561
doi: 10.1016/j.mehy.2013.12.024
Thapa L, He C, Chen H. Study on the expression of angiotensin II (ANG II) receptor subtype 1 (AT1R) in the placenta of pregnancy-induced hypertension. Placenta 2004;25:637–41.
pubmed: 15193870
doi: 10.1016/j.placenta.2004.01.026
Li XC, Zhang J, Zhuo JL. The vasoprotective axes of the renin-angiotensin system: physiological relevance and therapeutic implications in cardiovascular, hypertensive and kidney diseases. Pharmacol Res. 2017;125:21–38.
pubmed: 28619367
pmcid: 5607101
doi: 10.1016/j.phrs.2017.06.005
Naidoo N, Moodley J, Naicker T. Maternal endothelial dysfunction in HIV-associated preeclampsia comorbid with COVID-19: a review. Hypertens Res. 2021;44:386–98.
pubmed: 33469197
pmcid: 7815501
doi: 10.1038/s41440-020-00604-y
Mezoh G, Lutchman N, Worsley C, Gededzha M, Mayne E, Martinson N, et al. Biomarkers of endothelial activation in Black South African HIV-positive subjects are associated with both high viral load and low CD4 counts. AIDS Res Hum Retrovir. 2022;38:152–61.
pubmed: 34969264
doi: 10.1089/aid.2021.0052
Palakeel JJ, Ali M, Chaduvula P, Chhabra S, Lamichhane SL, Ramesh V, et al. An Outlook on the Etiopathogenesis of Pulmonary Hypertension in HIV. Cureus 2022;14:e27390.
pubmed: 36046315
pmcid: 9418639
David M, Naicker T. The complement system in preeclampsia: a review of its activation and endothelial injury in the triad of COVID 19 infection and HIV-associated preeclampsia. Obstet Gynecol Sci. 2023. https://doi.org/10.5468/ogs.22175 . Online ahead of print 2023/01/05.
David M, Moodley J, Naicker T. The function of adipsin and C9 protein in the complement system in HIV-associated preeclampsia. Arch Gynecol Obstet. 2021;304:1467–73.
pubmed: 33881585
doi: 10.1007/s00404-021-06069-9
Naicker T, Govender N, Abel T, Naidoo N, Moodley M, Pillay Y, et al. HIV associated preeclampsia: a multifactorial appraisal. Int J Mol Sci. 2021;22:9157.
pubmed: 34502066
pmcid: 8431090
doi: 10.3390/ijms22179157
Pillay Y, Moodley J, Naicker T. The role of the complement system in HIV infection and preeclampsia. Inflamm Res. 2019;68:459–69.
pubmed: 31028431
doi: 10.1007/s00011-019-01240-0
Govender S, Naicker T. The Contribution of Complement Protein C1q in COVID-19 and HIV Infection Comorbid with Preeclampsia: A Review. Int Arch Allergy Immunol. 2022;183:1114–26.
pubmed: 35661665
doi: 10.1159/000524976
Sikhosana ML, Suchard M, Kuonza L, Cutland C, Slogrove A, Otwombe K, et al. Association between preeclampsia and HIV: a case-control study in urban South Africa. AJOG Glob Rep. 2022;2:100056.
pubmed: 36276804
pmcid: 9563819
doi: 10.1016/j.xagr.2022.100056
Kalumba V, Moodley J, Naidoo T. Is the prevalence of pre-eclampsia affected by HIV/AIDS? A retrospective case-control study: cardiovascular topics. Cardiovasc J Afr. 2013;24:24–7.
pubmed: 23612949
pmcid: 3734876
doi: 10.5830/CVJA-2012-078
Sansone M, Sarno L, Saccone G, Berghella V, Maruotti GM, Migliucci A, et al. Risk of preeclampsia in human immunodeficiency virus–infected pregnant women. Obstet Gynecol. 2016;127:1027–32.
pubmed: 27159742
doi: 10.1097/AOG.0000000000001424
Pangmekeh PJ, Awolu MM, Gustave S, Gladys T, Cumber SN. Association between highly active antiretroviral therapy (HAART) and hypertension in persons living with HIV/AIDS at the Bamenda regional hospital, Cameroon. Pan Afr Med J. 2019;33:87.
pubmed: 31489065
pmcid: 6713512
doi: 10.11604/pamj.2019.33.87.15574
Chandel N, Ayasolla K, Lan X, Rai P, Mikulak J, Husain M, et al. Renin modulates HIV replication in T cells. J Leukoc Biol. 2014;96:601–9.
pubmed: 24970860
pmcid: 4163631
doi: 10.1189/JLB.2A0414-192R
Tzoupis H, Leonis G, Megariotis G, Supuran CT, Mavromoustakos T, Papadopoulos MG. Dual inhibitors for aspartic proteases HIV-1 PR and renin: advancements in AIDS–hypertension–diabetes linkage via molecular dynamics, inhibition assays, and binding free energy calculations. J Med Chem. 2012;55:5784–96.
pubmed: 22621689
doi: 10.1021/jm300180r
Woldesenbet SA, Kufa T, Barron P, Ayalew K, Cheyip M, Chirombo BC, et al. Assessment of readiness to transition from antenatal HIV surveillance surveys to PMTCT programme data-based HIV surveillance in South Africa: The 2017 Antenatal Sentinel HIV Survey. Int J Infect Dis. 2020;91:50–56.
pubmed: 31712090
doi: 10.1016/j.ijid.2019.11.005
Implementation of the universal test and treat strategy for HIV positive patients and differentiated care for stable patients 2016 2016. https://sahivsoc.org/Files/22%208%2016%20Circular%20UTT%20%20%20Decongestion%20CCMT%20Directorate.pdf .
Veerbeek JH, Uiterweer EP, Nikkels PG, Koenen SV, van der Zalm M, Koster M, et al. Biopsy techniques to study the human placental bed. Placenta 2015;36:775–82.
pubmed: 26076963
doi: 10.1016/j.placenta.2015.05.008
Slaoui M, Fiette L. Histopathology procedures: from tissue sampling to histopathological evaluation. Methods Mol Biol. 2011;691:69–82.
Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9:671–5.
pubmed: 22930834
pmcid: 5554542
doi: 10.1038/nmeth.2089
Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9:676–82.
pubmed: 22743772
doi: 10.1038/nmeth.2019
Leal CRV, Costa LB, Ferreira GC, de Melo Ferreira A, Reis FM. e Silva ACS. Renin-angiotensin system in normal pregnancy and in preeclampsia: A comprehensive review. Pregnancy Hypertens. 2022;28:15–20.
pubmed: 35149272
doi: 10.1016/j.preghy.2022.01.011
Irani RA, Xia Y. Renin angiotensin signaling in normal pregnancy and preeclampsia. InSeminars in nephrology 2011;31:47–58.
Dechend R, Lamarca B, Davidge STSalt. Aldosterone, and the Renin–Angiotensin System in Pregnancy. Chesley’s Hypertens Disord Pregnancy. 2022;5:335–53.
doi: 10.1016/B978-0-12-818417-2.00010-5
Rautureau Y, Paradis P, Schiffrin EL. Cross-talk between aldosterone and angiotensin signaling in vascular smooth muscle cells. Steroids 2011;76:834–9.
pubmed: 21371487
Tomimatsu T, Mimura K, Matsuzaki S, Endo M, Kumasawa K, Kimura T. Preeclampsia: maternal systemic vascular disorder caused by generalized endothelial dysfunction due to placental antiangiogenic factors. Int J Mol Sci. 2019;20:4246.
pubmed: 31480243
pmcid: 6747625
doi: 10.3390/ijms20174246
San Juan-Reyes S, Gómez-Oliván LM, Islas-Flores H, Dublán-García O. Oxidative stress in pregnancy complicated by preeclampsia. Arch Biochem Biophys. 2020;681:108255.
pubmed: 31904364
doi: 10.1016/j.abb.2020.108255
Hoch NE, Guzik TJ, Chen W, Deans T, Maalouf SA, Gratze P, et al. Regulation of T-cell function by endogenously produced angiotensin II. Am J Physiol-Regul Integr Comp Physiol. 2009;296:R208–R216.
pubmed: 19073907
doi: 10.1152/ajpregu.90521.2008
Campbell N, LaMarca B, Cunningham MW Jr. The role of agonistic autoantibodies to the angiotensin II type 1 receptor (AT1-AA) in pathophysiology of preeclampsia. Curr Pharm Biotechnol. 2018;19:781–5.
pubmed: 30255752
pmcid: 6596412
doi: 10.2174/1389201019666180925121254
Cunningham MW Jr, Vaka VR, McMaster K, Ibrahim T, Cornelius DC, Amaral L, et al. Renal natural killer cell activation and mitochondrial oxidative stress; new mechanisms in AT1-AA mediated hypertensive pregnancy. Pregnancy Hypertens. 2019;15:72–7.
pubmed: 30825931
doi: 10.1016/j.preghy.2018.11.004
Nirupama R, Divyashree S, Janhavi P, Muthukumar S, Ravindra P. Preeclampsia: Pathophysiology and management. J Gynecol Obstet Hum Reprod. 2021;50:101975.
pubmed: 33171282
doi: 10.1016/j.jogoh.2020.101975
Rüster C, Wolf G. Angiotensin II as a morphogenic cytokine stimulating renal fibrogenesis. J Am Soc Nephrol. 2011;22:1189–99.
pubmed: 21719784
doi: 10.1681/ASN.2010040384
Oro C, Qian H, Thomas WG. Type 1 angiotensin receptor pharmacology: signaling beyond G proteins. Pharmacol Ther. 2007;113:210–26.
pubmed: 17125841
doi: 10.1016/j.pharmthera.2006.10.001
Thapa S, Shrestha U. Immune Reconstitution Inflammatory Syndrome. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023.
Verma S, Pillay P, Naicker T, Moodley J, Mackraj I. Placental hypoxia inducible factor-1α & CHOP immuno-histochemical expression relative to maternal circulatory syncytiotrophoblast micro-vesicles in preeclamptic and normotensive pregnancies. Eur J Obstet Gynecol Reprod Biol. 2018;220:18–24.
pubmed: 29127866
doi: 10.1016/j.ejogrb.2017.11.004
Masiá M, Padilla S, Fernández M, Rodríguez C, Moreno A, Oteo JA, et al. Oxidative stress predicts all-cause mortality in HIV-infected patients. PloS ONE. 2016;11:e0153456.
pubmed: 27111769
pmcid: 4844170
doi: 10.1371/journal.pone.0153456
Mi S, Lee X, Li X-P, Veldman GM, Finnerty H, Racie L, et al. Syncytin is a captive retroviral envelope protein involved in human placental morphogenesis. Nature 2000;403:785–9.
pubmed: 10693809
doi: 10.1038/35001608
Anand AR, Rachel G, Parthasarathy D. HIV proteins and endothelial dysfunction: implications in cardiovascular disease. Front Cardiovasc Med. 2018;5:185.
pubmed: 30619892
pmcid: 6305718
doi: 10.3389/fcvm.2018.00185
Farinacci M, Krahn T, Dinh W, Volk HD, Düngen HD, Wagner J, et al. Circulating endothelial cells as biomarker for cardiovascular diseases. Res Pract Thromb Haemost. 2019;3:49–58.
pubmed: 30656276
doi: 10.1002/rth2.12158
Naicker T, Phoswa WN, Onyangunga OA, Gathiram P, Moodley J. Angiogenesis, lymphangiogenesis, and the immune response in South African preeclamptic women receiving HAART. Int J Mol Sci. 2019;20:3728.
pubmed: 31366152
pmcid: 6696390
doi: 10.3390/ijms20153728
Mediouni S, Darque A, Baillat G, Ravaux I, Dhiver C, Tissot-Dupont H, et al. Antiretroviral therapy does not block the secretion of the human immunodeficiency virus tat protein. Infect Disord-Drug Targets (Former Curr Drug Targets-Infect Disord). 2012;12:81–6.
Obimbo MM, Zhou Y, McMaster MT, Cohen CR, Qureshi Z, Ong’ech J, et al. Placental structure in preterm birth among HIV-positive versus HIV-negative women in Kenya. J Acquired Immune Defic Syndrom (1999). 2019;80:94.
doi: 10.1097/QAI.0000000000001871
Machado ES, Krauss MR, Megazzini K, Coutinho CM, Kreitchmann R, Melo VH, et al. Hypertension, preeclampsia and eclampsia among HIV-infected pregnant women from Latin America and Caribbean countries. J Infect. 2014;68:572–80.
pubmed: 24462561
pmcid: 4046640
doi: 10.1016/j.jinf.2013.12.018
Steckelings UM, Widdop RE, Sturrock ED, Lubbe L, Hussain T, Kaschina E, et al. The Angiotensin AT2 Receptor: From a Binding Site to a Novel Therapeutic Target. Pharmacol Rev. 2022;74:1051–135.
pubmed: 36180112
doi: 10.1124/pharmrev.120.000281
Savoia C, Ebrahimian T, He Y, Gratton J-P, Schiffrin EL, Touyz RM. Angiotensin II/AT2 receptor-induced vasodilation in stroke-prone spontaneously hypertensive rats involves nitric oxide and cGMP-dependent protein kinase. J Hypertens. 2006;24:2417–22.
pubmed: 17082724
doi: 10.1097/01.hjh.0000251902.85675.7e
Danyel LA, Schmerler P, Paulis L, Unger T, Steckelings UM. Impact of AT2-receptor stimulation on vascular biology, kidney function, and blood pressure. Integr Blood Press Control. 2013;6:153.
pubmed: 24379697
pmcid: 3873809
Masenga SK, Elijovich F, Koethe JR, Hamooya BM, Heimburger DC, Munsaka SM, et al. Hypertension and metabolic syndrome in persons with HIV. Curr Hypertens Rep. 2020;22:1–8.
doi: 10.1007/s11906-020-01089-3
Soltani-Zangbar MS, Pahlavani B, Zolghadri J, Gharesi-Fard B. Angiotensin type 2 receptor gene polymorphisms and susceptibility to preeclampsia. J Reprod Infertil. 2018;19:95.
pubmed: 30009143
pmcid: 6010826
Aung M, Konoshita T, Moodley J, Gathiram P. Association of gene polymorphisms of four components of renin-angiotensin-aldosterone system and preeclampsia in South African black women. Eur J Obstet Gynecol Reprod Biol. 2017;215:180–7.
pubmed: 28641212
doi: 10.1016/j.ejogrb.2017.05.011
Aung M, Konoshita T, Moodley J, Gathiram P. Association of gene polymorphisms of aldosterone synthase and angiotensin converting enzyme in pre-eclamptic South African Black women. Pregnancy Hypertens. 2018;11:38–43.
pubmed: 29523271
doi: 10.1016/j.preghy.2017.12.004
Zulli A, Burrell LM, Buxton BF, Hare DL. ACE2 and AT4R are present in diseased human blood vessels. Eur J Histochem. 2008;52:39–44.
pubmed: 18502721
doi: 10.4081/1184
Singh KD, Karnik SS. Angiotensin receptors: structure, function, signaling and clinical applications. J Cell Signal. 2016;1:1–8.
Williams P, Mistry H, Innes B, Bulmer J, Pipkin FB. Expression of AT1R, AT2R and AT4R and their roles in extravillous trophoblast invasion in the human. Placenta 2010;31:448–55.
pubmed: 20304486
doi: 10.1016/j.placenta.2010.02.014
Niwa M, Numaguchi Y, Ishii M, Kuwahata T, Kondo M, Shibata R, et al. IRAP deficiency attenuates diet-induced obesity in mice through increased energy expenditure. Biochem Biophys Res Commun. 2015;457:12–8.
pubmed: 25534853
doi: 10.1016/j.bbrc.2014.12.071
Khaliq OP, Konoshita T, Moodley J, Naicker T. Soluble angiotensin IV receptor levels in preeclampsia: is there a variation? J Matern-Fetal Neonatal Med. 2022;35:1156–61.
pubmed: 32208780
doi: 10.1080/14767058.2020.1743665
Khaliq OP, Konoshita T, Moodley J, Naicker T. The role of LNPEP and ANPEP gene polymorphisms in the pathogenesis of pre-eclampsia. Eur J Obstet Gynecol Reprod Biol. 2020;252:160–5.
pubmed: 32619880
doi: 10.1016/j.ejogrb.2020.06.037