Impact of adrenomedullin on mitochondrial respiratory capacity in human adipocyte.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 06 2023
13 06 2023
Historique:
received:
17
02
2023
accepted:
07
06
2023
medline:
15
6
2023
pubmed:
14
6
2023
entrez:
13
6
2023
Statut:
epublish
Résumé
Mitochondrial function in adipocyte is an important aspect in maintaining metabolic homeostasis. Our previous observation showed that circulating levels of adrenomedullin (ADM) and mRNA and protein for ADM in omental adipose tissue were higher in patients with gestational diabetes mellitus (GDM), and these alterations are accompanied by glucose and lipid metabolic dysregulation, but the impact of ADM on mitochondrial biogenesis and respiration in human adipocyte remain elusive. The present study demonstrated that: (1) Increasing doses of glucose and ADM inhibit human adipocyte mRNA expressions of mitochondrial DNA (mtDNA)-encoded subunits of electron transport chain, including nicotinamide adenine dinucleotide dehydrogenase (ND) 1 and 2, cytochrome (CYT) b, as well as ATPase 6; (2) ADM significantly increases human adipocyte mitochondrial reactive oxygen species generation and this increase is reversed by ADM antagonist, ADM22-52, but treatment with ADM does not significantly affect mitochondrial contents in the adipocytes; (3) Adipocyte basal and maximal oxygen consumption rate are dose-dependently suppressed by ADM, thus results in impaired mitochondrial respiratory capacity. We conclude that elevated ADM observed in diabetic pregnancy may be involved in glucose and lipid dysregulation through compromising adipocyte mitochondrial function, and blockade of ADM action may improve GDM-related glucose and adipose tissue dysfunction.
Identifiants
pubmed: 37311963
doi: 10.1038/s41598-023-36622-2
pii: 10.1038/s41598-023-36622-2
pmc: PMC10264416
doi:
Substances chimiques
Adrenomedullin
148498-78-6
Cytochromes b
9035-37-4
DNA, Mitochondrial
0
Glucose
IY9XDZ35W2
Lipids
0
RNA, Messenger
0
ADM protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
9578Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL058144
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD091503
Pays : United States
Organisme : NIH HHS
ID : HD091503 and HL58144 to CY
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023. The Author(s).
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