Assessment of genetic polymorphisms associated with malaria antifolate resistance among the population of Libreville, Gabon.

Humans Folic Acid Antagonists Gabon / epidemiology Malaria Malaria, Falciparum / epidemiology Polymorphism, Single Nucleotide Artemisinins
Artemisinin combination therapy Haplotypes Libreville Malaria Mutation Resistance

Journal

Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802

Informations de publication

Date de publication:
14 Jun 2023
Historique:
received: 10 02 2023
accepted: 04 06 2023
medline: 15 6 2023
pubmed: 14 6 2023
entrez: 13 6 2023
Statut: epublish

Résumé

Gabon is a malaria-threatened country with a stable and hyperendemic transmission of Plasmodium falciparum monoinfection. Malaria drug resistance is widely spread in many endemic countries around the world, including Gabon. The molecular surveillance of drug resistance to antifolates and artemisinin-based combination therapy (ACT) is one of the strategies for combating malaria. As Plasmodium parasites continue to develop resistance to currently available anti-malarial drugs, this study evaluated the frequency of the polymorphisms and genetic diversity associated with this phenomenon among the parasites isolates in Gabon. To assess the spread of resistant haplotypes among the malaria-infected population of Libreville, single nucleotide polymorphisms linked to sulfadoxine-pyrimethamine (SP) and artemisinin drugs resistance were screened for P. falciparum dihydrofolate reductase (Pfdhfr), P. falciparum dihydropteroate synthase (Pfdhps), and P. falciparum kelch 13-propeller domain (Pfk13) point mutations. The analysis of 70 malaria-positive patient samples screened for polymorphism showed 92.65% (n = 63) mutants vs. 7.35% (n = 5) wild parasite population in Pfdhfr, with high prevalence mutations at S High polymorphism frequencies of Pfdhfr and Pfdhps genes were observed, with alternative alanine/phenylalanine mutation at S

Sections du résumé

BACKGROUND BACKGROUND
Gabon is a malaria-threatened country with a stable and hyperendemic transmission of Plasmodium falciparum monoinfection. Malaria drug resistance is widely spread in many endemic countries around the world, including Gabon. The molecular surveillance of drug resistance to antifolates and artemisinin-based combination therapy (ACT) is one of the strategies for combating malaria. As Plasmodium parasites continue to develop resistance to currently available anti-malarial drugs, this study evaluated the frequency of the polymorphisms and genetic diversity associated with this phenomenon among the parasites isolates in Gabon.
METHODS METHODS
To assess the spread of resistant haplotypes among the malaria-infected population of Libreville, single nucleotide polymorphisms linked to sulfadoxine-pyrimethamine (SP) and artemisinin drugs resistance were screened for P. falciparum dihydrofolate reductase (Pfdhfr), P. falciparum dihydropteroate synthase (Pfdhps), and P. falciparum kelch 13-propeller domain (Pfk13) point mutations.
RESULTS RESULTS
The analysis of 70 malaria-positive patient samples screened for polymorphism showed 92.65% (n = 63) mutants vs. 7.35% (n = 5) wild parasite population in Pfdhfr, with high prevalence mutations at S
CONCLUSIONS CONCLUSIONS
High polymorphism frequencies of Pfdhfr and Pfdhps genes were observed, with alternative alanine/phenylalanine mutation at S

Identifiants

DOI: 10.1186/s12936-023-04615-1 PMID: 37312220 PMC: PMC10265907
pubmed: 37312220
doi: 10.1186/s12936-023-04615-1
pii: 10.1186/s12936-023-04615-1
pmc: PMC10265907
doi:

Substances chimiques

Folic Acid Antagonists 0
Artemisinins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

183

Subventions

Organisme : National Research Foundation of Korea (NRF) Brain Pool program
ID : NRF-2018H1D3A1A02074759
Organisme : National Research Foundation of Korea (NRF) Basic Science Research Program
ID : NRF-2019R1C1C1002170
Organisme : Framework of the international cooperation program
ID : 2022K2A9A1A01098057, FY2022

Informations de copyright

© 2023. The Author(s).

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Auteurs

Sylvatrie-Danne Dinzouna-Boutamba (SD)

Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, Korea.

Berthe Amélie Iroungou (BA)

Unité Mixte de Recherche Centre International de Recherches Médicales de Franceville et le Service de Santé Militaire, Libreville, 20404, Gabon.

Falone Larissa Akombi (FL)

Unité Mixte de Recherche Centre International de Recherches Médicales de Franceville et le Service de Santé Militaire, Libreville, 20404, Gabon.

Lauriane Yacka-Mouele (L)

Unité Mixte de Recherche Centre International de Recherches Médicales de Franceville et le Service de Santé Militaire, Libreville, 20404, Gabon.

Zin Moon (Z)

Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, Korea.

Ja Moon Aung (JM)

Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, Korea.

Sanghyun Lee (S)

Division of Bio Bigdata, Department of Precision Medicine, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju, 28159, Korea.

Dong-Il Chung (DI)

Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, Korea.

Yeonchul Hong (Y)

Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, Korea.

Youn-Kyoung Goo (YK)

Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, Korea. kuku1819@knu.ac.kr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Assessment of genetic polymorphisms associated...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Mécanismes moléculaires de l'action pharmacologique › Antienzymes › Antifoliques › Assessment of genetic polymorphisms associated...
questionsmedicales.fr › Régions géographiques › Afrique › Afrique subsaharienne › Afrique centrale › Gabon › Assessment of genetic polymorphisms associated...
questionsmedicales.fr › Infection › Maladies vectorielles › Paludisme › Assessment of genetic polymorphisms associated...
questionsmedicales.fr › Infection › Maladies vectorielles › Paludisme › Paludisme à Plasmodium falciparum › Assessment of genetic polymorphisms associated...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Polymorphisme génétique › Polymorphisme de nucléotide simple › Assessment of genetic polymorphisms associated...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Terpènes › Sesquiterpènes › Artémisinines › Assessment of genetic polymorphisms associated...