Loricrin and Cytokeratin Disorganisation in Severe Forms of Periodontitis.
Epithelial barrier
Loricrin
Periodontal disease
Stat6VT
Journal
International dental journal
ISSN: 1875-595X
Titre abrégé: Int Dent J
Pays: England
ID NLM: 0374714
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
30
12
2022
revised:
05
05
2023
accepted:
22
05
2023
medline:
3
11
2023
pubmed:
15
6
2023
entrez:
14
6
2023
Statut:
ppublish
Résumé
The aim of this research was to investigate the role of the cornified epithelium, the outermost layer of the oral mucosa, engineered to prevent water loss and microorganism invasion, in severe forms of periodontitis (stage III or IV, grade C). Porphyromonas gingivalis, a major periodontal disease pathogen, can affect cornified epithelial protein expression through chronic activation of signal transducer and activator of transcription 6 (Stat6). We used a mouse model, Stat6VT, that mimics this to determine the effects of barrier defect on P gingivalis-induced inflammation, bone loss, and cornified epithelial protein expression, and compared histologic and immunohistologic findings with tissues obtained from human controls and patients with stage III and IV, grade C disease. Alveolar bone loss in mice was assessed using micro-computerised tomography, and soft tissue morphology was qualitatively and semi-quantitatively assessed by histologic examination for several proteins, including loricrin, filaggrin, cytokeratin 1, cytokeratin 14, a proliferation marker, a pan-leukocyte marker, as well as morphologic signs of inflammation. Relative cytokine levels were measured in mouse plasma by cytokine array. In the tissues from patients with periodontal disease, there were greater signs of inflammation (rete pegs, clear cells, inflammatory infiltrates) and a decrease and broadening of expression of loricrin and cytokeratin 1. Cytokeratin 14 expression was also broader and decreased in stage IV. P gingivalis-infected Stat6VT mice showed greater alveolar bone loss in 9 out of 16 examined sites, and similar patterns of disruption to human patients in expression of loricrin and cytokeratins 1 and 14. There were also increased numbers of leukocytes, decreased proliferation, and greater signs of inflammation compared with P gingivalis-infected control mice. Our study provides evidence that changes in epithelial organisation can exacerbate the effects of P gingivalis infection, with similarities to the most severe forms of human periodontitis.
Identifiants
pubmed: 37316411
pii: S0020-6539(23)00089-8
doi: 10.1016/j.identj.2023.05.004
pmc: PMC10658443
pii:
doi:
Substances chimiques
loricrin
0
Keratin-14
0
Keratins
68238-35-7
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
862-872Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest None disclosed.
Références
Invest Ophthalmol Vis Sci. 2014 May 20;55(6):3803-8
pubmed: 24845637
Microbes Infect. 2005 Feb;7(2):157-63
pubmed: 15716056
Blood. 2007 Dec 15;110(13):4367-9
pubmed: 17878403
BMC Oral Health. 2019 Mar 28;19(1):52
pubmed: 30922293
Infect Immun. 2006 Feb;74(2):1204-14
pubmed: 16428770
Pathog Dis. 2018 Jun 1;76(4):
pubmed: 29635433
J Cell Commun Signal. 2009 Dec;3(3-4):311-22
pubmed: 19798593
J Periodontol. 2005 Aug;76(8):1282-6
pubmed: 16101359
Prog Orthod. 2018 Jun 25;19(1):18
pubmed: 29938297
Immunol Res. 2011 May;50(1):87-96
pubmed: 21442426
Br J Dermatol. 2008 Sep;159(3):714-9
pubmed: 18844868
Oral Dis. 2015 Apr;21(3):314-9
pubmed: 24989645
Gene. 2017 Apr 15;608:13-19
pubmed: 28111259
N Engl J Med. 2004 Nov 11;351(20):2087-100
pubmed: 15537907
Front Pharmacol. 2019 Nov 22;10:1251
pubmed: 31824303
J Invest Dermatol. 2011 Jun;131(6):1272-9
pubmed: 21346775
J Oral Pathol Med. 2001 Feb;30(2):104-12
pubmed: 11168855
J Periodontal Res. 1994 Jan;29(1):46-53
pubmed: 7509385
Med Oral Patol Oral Cir Bucal. 2014 Jul 01;19(4):e359-65
pubmed: 24608215
PLoS One. 2015 May 04;10(5):e0125126
pubmed: 25938460
Clin Immunol. 2008 Mar;126(3):332-7
pubmed: 18166499
J Immunol. 2008 Mar 1;180(5):3551-9
pubmed: 18292582
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10754-8
pubmed: 8248167
Infect Immun. 2002 May;70(5):2512-8
pubmed: 11953390
Front Endocrinol (Lausanne). 2018 Feb 16;9:31
pubmed: 29503631
Imaging Sci Dent. 2021 Dec;51(4):389-398
pubmed: 34987999
Br J Dermatol. 2005 Jan;152(1):146-9
pubmed: 15656815
Int J Mol Sci. 2020 May 31;21(11):
pubmed: 32486523
Eur J Immunol. 2016 Nov;46(11):2609-2613
pubmed: 27510401
PLoS One. 2014 Jun 24;9(6):e100838
pubmed: 24959715
J Bone Miner Res. 2014 Nov;29(11):2427-38
pubmed: 24839202
J Immunol. 2003 Apr 1;170(7):3478-87
pubmed: 12646608
Mol Immunol. 2010 Jan;47(4):701-5
pubmed: 19959237
Adv Protein Chem Struct Biol. 2020;120:45-84
pubmed: 32085888
J Clin Periodontol. 2011 Jul;38(7):599-611
pubmed: 21501207
Front Oncol. 2014 May 19;4:96
pubmed: 24904820
Arch Oral Biol. 2021 Jun;126:105129
pubmed: 33934042
Cell. 1991 Jan 25;64(2):365-80
pubmed: 1703046
Front Biosci. 2007 May 01;12:3965-74
pubmed: 17485350
J Allergy Clin Immunol. 2008 Oct;122(4):689-693
pubmed: 18774165
Arterioscler Thromb Vasc Biol. 2003 Aug 1;23(8):1405-11
pubmed: 12816879
Ann Hum Genet. 2004 Nov;68(Pt 6):639-45
pubmed: 15598222
J Oral Maxillofac Pathol. 2015 Jan-Apr;19(1):64-8
pubmed: 26097310
Sci Rep. 2018 Jun 13;8(1):9008
pubmed: 29899364
J Indian Soc Periodontol. 2011 Oct;15(4):318-22
pubmed: 22368353
J Clin Periodontol. 2007 Jun;34(6):473-9
pubmed: 17451413
Open Life Sci. 2019 Dec 31;14:638-646
pubmed: 33817202
Cell Physiol Biochem. 2018;49(3):884-898
pubmed: 30184545
Front Microbiol. 2016 Feb 09;7:53
pubmed: 26903954
Methods Mol Biol. 2017;1641:101-114
pubmed: 28748459
Nat Rev Mol Cell Biol. 2005 Apr;6(4):328-40
pubmed: 15803139
Elife. 2021 Jan 04;10:
pubmed: 33393902
J Immunol. 2013 Mar 1;190(5):2282-91
pubmed: 23365081
Asian Pac J Cancer Prev. 2018 Nov 29;19(11):3279-3286
pubmed: 30486632
Exp Mol Med. 1999 Mar 31;31(1):5-19
pubmed: 10231017
J Cell Biol. 2000 Oct 16;151(2):401-12
pubmed: 11038186
Int J Oral Sci. 2018 Jan 10;10(1):e8
pubmed: 29319048
J Periodontol. 2018 Jun;89 Suppl 1:S1-S8
pubmed: 29926946
Innate Immun. 2017 Nov;23(8):641-647
pubmed: 28952836
J Periodontal Res. 2010 Dec;45(6):803-8
pubmed: 20860589
Int J Mol Sci. 2021 Jun 10;22(12):
pubmed: 34200555
Sci Rep. 2018 Aug 6;8(1):11720
pubmed: 30082828
Mediators Inflamm. 2000;9(2):115-20
pubmed: 10958385
Histol Histopathol. 1998 Jul;13(3):819-26
pubmed: 9690138
J Dtsch Dermatol Ges. 2011 Sep;9(9):670-6
pubmed: 21518425
J Dtsch Dermatol Ges. 2019 Dec;17(12):1227-1238
pubmed: 31846220
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10869-74
pubmed: 11553815
Nat Genet. 1996 May;13(1):70-7
pubmed: 8673107
J Bone Miner Res. 2004 Jul;19(7):1075-83
pubmed: 15176989
Arch Dermatol Res. 2018 Apr;310(3):197-207
pubmed: 29368135
J Invest Dermatol. 2004 Apr;122(4):909-22
pubmed: 15102081
Crit Rev Oral Biol Med. 1990;1(3):167-90
pubmed: 1717003
J Oral Maxillofac Pathol. 2014 May;18(2):169-76
pubmed: 25328294
J Periodontal Res. 2017 Feb;52(1):42-50
pubmed: 27016120
J Invest Dermatol. 2011 Mar;131(3):791-4
pubmed: 21191418
Int Endod J. 2020 Jun;53(6):812-823
pubmed: 32053244
Int J Pharm. 2012 Oct 1;435(1):3-9
pubmed: 22705878
Infect Immun. 2000 Mar;68(3):1441-9
pubmed: 10678958
J Pathol. 2004 Nov;204(4):355-66
pubmed: 15495218
Arch Dermatol Res. 2009 Jul;301(6):397-404
pubmed: 19301019
Cell Microbiol. 2013 Oct;15(10):1722-34
pubmed: 23551616
J Periodontol. 2011 Jan;82(1):86-95
pubmed: 20809862
J Oral Microbiol. 2020 Mar 26;12(1):1742528
pubmed: 32341760
Bioessays. 2002 Sep;24(9):789-800
pubmed: 12210515
Int Arch Allergy Immunol. 2010;151(1):28-37
pubmed: 19672094
J Periodontol. 2016 Jan;87(1):58-65
pubmed: 26136121
J Contemp Dent Pract. 2014 Jan 01;15(1):127-36
pubmed: 24939280
Quintessence Int. 2018;49(3):173-180
pubmed: 29363676
Front Endocrinol (Lausanne). 2018 Apr 09;9:117
pubmed: 29686648
J Invest Dermatol. 2002 Jan;118(1):102-9
pubmed: 11851882