Panel of miR-150 and linc00673, regulators of CCR6/CCL20 may serve as non-invasive diagnostic marker of non-small cell lung cancer.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 06 2023
14 06 2023
Historique:
received:
24
02
2023
accepted:
05
06
2023
medline:
16
6
2023
pubmed:
15
6
2023
entrez:
14
6
2023
Statut:
epublish
Résumé
The C-C motif ligand 20 (CCL20) is a chemokine that specifically binds to the chemokine receptor 6 (CCR6) and the CCL20/CCR6 axis has been implicated in the non-small lung cancer (NSCLC) development and progression. Its expression is regulated by mutual interactions of non-coding RNAs (ncRNAs). This goals of presented study was to evaluate the expression level of CCR6/CCL20 mRNA in NSCLC tissue comparative to selected ncRNAs: miR-150, linc00673. The expression level of the studied ncRNAs was also assessed in serum extracellular vesicles (EVs). Thirty patients (n = 30) were enrolled as the study cohort. Total RNA was isolated from tumor tissue, adjacent macroscopically unchanged tissue and serum EVs. The expression level of studied genes and ncRNAs were estimated based on the qPCR method. Higher expression level of CCL20 mRNA but lower expression level of CCR6 mRNA were observed in tumor in comparison to control tissue. Relative to the smoking status, higher CCL20 (p < 0.05) and CCR6 mRNA (p > 0.05) expression levels were observed in current smokers than in never smokers. In serum EVs the expression level of miR-150 has a negative correlation with AJCC tumor staging, whereas the expression level of linc00673 positively correlated (p > 0.05). The lower expression level of miR-150 and higher expression level of linc00673 in serum EVs were observed in NSCLC patients with lymph nodes metastases (p > 0.05). Regarding the histopathological type, significantly lower expression level of miR-150 and higher expression level of linc00673 were observed in the serum EVs of patients with AC compared to patient with SCC. Our findings revealed that smoking significantly changed the expression level of CCL20 mRNA in NSCLC tissue. Changes in expression levels of miR-150 and linc00673 in the serum EVs of NSCLC patients in relation to presence of lymph node metastases and the stage of cancer development may serve as a non-invasive molecular biomarkers of tumor progression. Furthermore, expression levels of miR-150 and linc00673 may serve as non-intrusive diagnostic biomarkers differentiating adenocarcinoma from squamous cell carcinoma.
Identifiants
pubmed: 37316552
doi: 10.1038/s41598-023-36485-7
pii: 10.1038/s41598-023-36485-7
pmc: PMC10267173
doi:
Substances chimiques
RNA, Messenger
0
MicroRNAs
0
CCR6 protein, human
0
Receptors, CCR6
0
CCL20 protein, human
0
Chemokine CCL20
0
MIRN150 microRNA, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9642Informations de copyright
© 2023. The Author(s).
Références
Front Cell Dev Biol. 2021 Apr 07;9:643942
pubmed: 33898432
Clin Exp Immunol. 2020 Feb;199(2):230-243
pubmed: 31631328
Acta Cytol. 2019;63(6):449-455
pubmed: 31091522
Cell Rep. 2016 May 31;15(9):2025-37
pubmed: 27210747
Int J Mol Sci. 2019 Mar 31;20(7):
pubmed: 30935143
Cancer Gene Ther. 2007 Oct;14(10):847-57
pubmed: 17599091
Contemp Oncol (Pozn). 2021;25(1):45-52
pubmed: 33911981
Immunology. 2003 Jul;109(3):365-73
pubmed: 12807482
Genomics Inform. 2020 Dec;18(4):e36
pubmed: 33412752
Toxicol Lett. 2020 Mar 1;320:37-45
pubmed: 31778776
RNA Biol. 2018;15(12):1499-1511
pubmed: 30499379
Int J Clin Exp Pathol. 2015 Jan 01;8(1):842-6
pubmed: 25755784
Oncol Lett. 2017 Dec;14(6):8183-8189
pubmed: 29250193
Biomed Pharmacother. 2015 Feb;69:242-8
pubmed: 25661365
Sci Rep. 2016 Dec 15;6:39001
pubmed: 27976702
Jpn J Clin Oncol. 2012 Mar;42(3):189-95
pubmed: 22210923
Mol Ther Nucleic Acids. 2019 Jun 7;16:675-685
pubmed: 31121479
Tumour Biol. 2011 Feb;32(1):197-202
pubmed: 20872189
J Cancer. 2015 Aug 07;6(10):938-52
pubmed: 26316890
J Leukoc Biol. 2007 Oct;82(4):946-55
pubmed: 17615381
J Clin Med. 2022 Jan 27;11(3):
pubmed: 35160116
Cancer Lett. 2015 Jul 10;363(1):60-70
pubmed: 25864589
FEBS Lett. 2013 Aug 2;587(15):2346-51
pubmed: 23747308
Cancers (Basel). 2019 Apr 30;11(5):
pubmed: 31052206
Glia. 2012 May;60(5):771-81
pubmed: 22319003
J Cell Mol Med. 2016 May;20(5):920-9
pubmed: 26968871
Int J Biol Sci. 2022 Jun 27;18(11):4275-4288
pubmed: 35864953
Tumour Biol. 2014 Jun;35(6):5287-93
pubmed: 24532468
Br J Cancer. 2020 Sep;123(6):942-954
pubmed: 32601464
Int J Med Sci. 2021 Jan 1;18(2):419-431
pubmed: 33390811
Int J Cancer. 2003 Jun 10;105(2):186-9
pubmed: 12673677
J Clin Invest. 2000 May;105(10):1383-93
pubmed: 10811846
Respiration. 2011;81(4):265-84
pubmed: 21430413
J Exp Clin Cancer Res. 2020 Jun 20;39(1):117
pubmed: 32563270
Int J Oncol. 2018 Mar;52(3):913-924
pubmed: 29286099
Oncol Rep. 2013 Feb;29(2):704-12
pubmed: 23228962
Thorax. 2015 May;70(5):501-3
pubmed: 25550385
Front Genet. 2021 Nov 12;12:758591
pubmed: 34868237
J Mol Diagn. 2015 May;17(3):209-24
pubmed: 25908243
Front Mol Biosci. 2022 Apr 11;9:807497
pubmed: 35480896
Diagnostics (Basel). 2022 Jul 01;12(7):
pubmed: 35885514
World J Radiol. 2012 Apr 28;4(4):128-34
pubmed: 22590666
Oncotarget. 2016 May 3;7(18):25558-75
pubmed: 27027352
Oxid Med Cell Longev. 2021 Jul 16;2021:5563746
pubmed: 34336101
Carcinogenesis. 2022 Nov 23;43(10):930-940
pubmed: 35767307
Cancer Res. 2004 Mar 1;64(5):1861-6
pubmed: 14996750
Nat Rev Immunol. 2002 May;2(5):372-7
pubmed: 12033743
Hum Exp Toxicol. 2018 Sep;37(9):920-928
pubmed: 29205062
Oncol Lett. 2015 Jul;10(1):11-16
pubmed: 26170969
Clin Lung Cancer. 2010 May;11(3):187-91
pubmed: 20439195
FEBS J. 2018 Aug;285(16):2944-2971
pubmed: 29637711
PLoS One. 2022 Mar 15;17(3):e0264645
pubmed: 35290415
Genes (Basel). 2016 Dec 26;8(1):
pubmed: 28035947
Cancer Inform. 2022 Feb 7;21:11769351221076062
pubmed: 35153470
Mol Aspects Med. 2019 Dec;70:3-20
pubmed: 30102929
Dis Markers. 2021 Nov 24;2021:5917506
pubmed: 34868395
PLoS One. 2011;6(9):e24856
pubmed: 21949768
Mol Cancer. 2017 Jul 11;16(1):118
pubmed: 28697764
Nat Rev Mol Cell Biol. 2021 Feb;22(2):96-118
pubmed: 33353982
Eur Respir J. 2014 Aug;44(2):361-70
pubmed: 24627531
Mol Biol Rep. 2019 Oct;46(5):5389-5396
pubmed: 31463641
Genes (Basel). 2022 Jun 29;13(7):
pubmed: 35885958