Analyses of Elaeocarpus sphaericus Extract for Antioxidant, Antiproliferative and Gene Repression Activities against HIF-1α and VEGF.
Elaeocarpus sphaericus
HIF-1 alpha
RT-PCR
VEGF
anticancer activity
antioxidant activity
Journal
Chemistry & biodiversity
ISSN: 1612-1880
Titre abrégé: Chem Biodivers
Pays: Switzerland
ID NLM: 101197449
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
20
02
2023
accepted:
15
06
2023
medline:
24
8
2023
pubmed:
15
6
2023
entrez:
15
6
2023
Statut:
ppublish
Résumé
The study presents antioxidant, phytochemical, anti-proliferative, and gene repression activities against Hypoxia-inducible factor (HIF-1) alpha and Vascular endothelial growth factor (VEGF) of Elaeocarpus sphaericus extract. Elaeocarpus sphaericus dried and crushed plant leaves were extracted using water and methanol by ASE (Accelerated Solvent Extraction) method. Total phenolic content (TPC) and total flavonoid content (TFC) were used to measure the extracts' phytochemical activity (TFC). Antioxidant potential of the extracts was measured through DPPH, ABTS, FRAP, and TRP. Methanolic extract of the leaves of E. sphaericus has shown a higher amount of TPC (94.666±4.040 mg/gm GAE) and TFC value (172.33±3.21 mg/gm RE). The antioxidant properties of extracts in the yeast model (Drug Rescue assay) showed promising results. Ascorbic acid, gallic acid, hesperidin, and quercetin were found in the aqueous and methanolic extracts of E. sphaericus at varying amounts, according to a densiometric chromatogram generated by HPTLC analysis. Methanolic extract of E. sphaericus (10 mg/ml) has shown good antimicrobial potential against all bacterial strains used in the study except E. coli. The anticancer activity of the extract in HeLa cell lines ranged from 77.94±1.03 % to 66.85±1.95 %, while it ranged from 52.83±2.57 % to 5.44 % in Vero cell lines at varying concentration (1000 μg/ml-31.2 μg/ml). A promising effect of extract was observed on the expression activity of HIF-1 and VEGF gene through RT-PCR assay.
Identifiants
pubmed: 37318911
doi: 10.1002/cbdv.202300249
doi:
Substances chimiques
Antioxidants
0
Vascular Endothelial Growth Factor A
0
Plant Extracts
0
Flavonoids
0
Methanol
Y4S76JWI15
Phenols
0
Phytochemicals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300249Subventions
Organisme : Director DIPAS, DRDO, Delhi
Organisme : Department of Biotechnology, Graphic Era Deemed to be University, Dehradun
Informations de copyright
© 2023 Wiley-VHCA AG, Zurich, Switzerland.
Références
E. Birben, U. M. Sahiner, C. Sackesen, S. Erzurum, O. Kalayci, World Allergy Organ. J. 2012, 5, 9-19. https://doi.org/10.1097/WOX.0b013e3182439613.
WHO Report: World cancer report, Edited by Bernard W. Stewart and Christopher P. Wild, 2014 IARC Publication.
A. Morales-Sanchez, Viruses 2014, 6, 4047-4079. doi: 10.3390/v6104047.
A. Giaccia, B. G. Slim, Nat. Rev. Drug Discovery 2003, 2, 803-811. https://doi.org/10.1038/nrd1199.
S. Patiar, A. L. Harris, Endocr.-Relat. Cancer 2006, 13, S61-S75. https://doi.org/10.1677/erc.1.01290.
J. A. Forsythe, B. H. Jiang, N. V. Iyer, F. Agani, S. W. Leung, R. D. Koos, G. L. Semenza, Mol. Cell. Biol. 1996, 16, 4604-4613. DOI: 10.1128/MCB.16.9.4604.
S. M. Sagar, D. Yance, R. K. Wong, Current Oncology. 2006, 13, 14-26. https://doi.org/10.3747/co.v13i1.77.
A. L. Demain, P. Vaishnav, Microb. Biotechnol. 2010, 4, 687-699. 10.1111/j.1751-7915.2010.00221.x.
R. K. Singh, G. Nath, Phytother. Res. 1999, 13, 448-450. DOI: 10.1002/(sici)1099-1573(199908/09)13, 5<448::aid-ptr480>3.0.co;2-6.
R. K. Singh, S. B. Acharya, S. K. Bhattacharya, Phytother. Res. 2000, 14, 36-39. DOI: 10.1002/(sici)1099-1573(200002)14, 1<36::aid-ptr541>3.0.co;2-j.
A. R. Juvear, S. S. Sakat, S. S. Wankhede, V. R. Mali, S. L. Bodhankar, Int. J. PharmTech Res. 2009, 1, 779-982. http://www.sphinxsai.com/PTVOL3/PT=59.
G. Shah, S. Richa, M. Avninder, R. Sandeep, P. Vivek, Int. J. PharmTech Res. 2010, 2, 1781-1786.
B. Singh, A. Chopra, T. Raj, Indian J. Pharm. Sci. 2010, 72, 261-265. DOI:10.4103/0250-474X.65021.
K. V. B. Rao, G. Kumar, L. Karthik, Bio. Tech. 2011, 40, 5384-5387.
S. E. Sudradjat, K. Herawan Timotius, Phytomedicine Plus 2022, 2 (4), 100365, https://doi.org/10.1016/j.phyplu.2022.100365.
A. B. Mahajanakatti, T. S. Deepak, R. R. Achar, S. Pradeep, S. K. Prasad, R. Narayanappa, D. Bhaskar, S. Shetty, G. Melappa, L. Chandramouli, S. Mazumdar, E. Silina, V. Stupin, C. Srinivasa, C. Shivamallu, S. P. Kollur, Molecules 2022, 27, 2442. https://doi.org/10.3390/molecules27082442.
A. Dasgupta, S. S. Agarwal, D. K. Basu, Indian J. Physiol. Pharmacol. 1984, 28, 245-246. PMID: 6519771.
T. S. Kumar, S. Shanmugam, T. Palvannam, V. V. Bharathi Kumar, IJPR 2008, 7, 211-215. 10.22037/IJPR.2010.767.
B. Singh, M. P. S. Ishar, J. Pharmacogn. Phytochem. 2013, 1, 117-121.
J. Lu, K. Zhang, S. Chen, W. Wen, Carcinogenesis 2009, 30, 636-644. doi: 10.1093/carcin/bgp009.
P. Karna, S. Chagani, S. R. Gundala, P. C. Rida, G. Asif, V. Sharma, M. V. Gupta, R. Aneja, Br. J. Nutr. 2012, 107, 473-484. DOI: 10.1017/S0007114511003308.
E. Solowey, M. Lichtenstein, S. Sallon, H. Paavilainen, E. Solowey, H. Lorberboum-Galski, TheScientificWorld 2014, 2014, 1-12. https://doi.org/10.1155/2014/721402.
H. Wagner, G. Ulrich-Merzenich, Science Direct 2008, 16, 97-110. DOI: 10.1016/j.phymed.2008.12.018.
P. Rasoanaivo, C. W. Wright, M. L. Willcox, B. Gilber, Malar. J. 2011, 10, 1-33. https://doi.org/10.1186/1475-2875-10-S1-S4.
B. Pandey, M. Rajbhandari, Nepal J. Sci. Technol. 2014, 15, 53-60. DOI:10.3126/njst.v15i1.12010.
B. C. Nandy, S. Hardainiyan, R. Saxena, Int. J. Pharm. Sci. Rev. Res. 2015, 7, 415-418. https://innovareacademics.in/journals/index.php/ijpps/article/view/5113.
A. V. A. David, R. Arulmoli, S. Parsuraman, Pharmacogn. Rev. 2016, 10, 84-89. doi: 10.4103/0973-7847.194044.
J. Dai, R. J. Mumper, Molecules 2010, 15, 7313-7352. doi: 10.3390/molecules15107313.
P. Lakhanpal, D. K. Rai, I. J. M. U 2007, 2, 20-35. DOI: 10.4314/ijmu.v2i2.39851.
M. K. Walingo, AJFAND 2005, 5, 1-13. DOI: 10.18697/ajfand.8.1155.
A. Lupulescu, Int. J. Vitam. Nutr. Res. 1994, 64, 3-14.
S. Verma, A. Singh, A. Mishra, Environ. Toxicol. Pharmacol. 2013, 35, 473-485. https://doi.org/10.1016/j.etap.2013.02.011.
A. Ahmadi, S. J. Hosseinimehr, F. Naghshvar, E. Hajir, M. Ghahremani, Arch. Pharmacal Res. 2008, 31, 794-797. https://doi.org/10.1007/s12272-001-1228-z.
N. S. M. B. A. Nayeem, H. Salem, S. Ahel-Alfqy, J. Appl. Pharmacol. 2016, 8, 1-4. DOI: 10.4172/1920-4159.1000213.
H. Parhiz, A. Roohbakhsh, F. Soltani, R. Rezaee, M. Iranshahi, Phytother. Res. 2015, 9, 323-331. https://doi.org/10.1002/ptr.5256.
R. Bos, H. Zhong, C. F. Hanrahan, E. C. Mommers, G. L. Semenza, H. M. Pinedo, M. D. Abeloff, J. W. Simons, P. J. Van Diest, E. Van der Wall, J. Natl. Cancer Inst. 2000, 93, 309-314. https://doi.org/10.1093/jnci/93.4.309.
A. Sharma, S. Joshi, N. Kumar, IJGP 2015, 9, 246-251. DOI: https://doi.org/10.22377/ijgp.v9i4.570.
A. L. Harris, Nat. Rev. Cancer 2002, 2, 38-47. DOI: 10.1038/nrc704.
G. L. Semenza, Nat. Rev. Cancer 2003, 3, 721-732. DOI: 10.1038/nrc1187.
S. J. Welsh, J. Powis, Curr. Cancer Drug Targets 2003, 3, 391-405. DOI: 10.2174/1568009033481732.
A. Hoeben, B. Landuyt, M. S. Highley, H. Wildiers, A. V. Van Oosterom, E. A. De Bruijn, Pharmacol. Rev. 2004, 56, 549-580. DOI: 10.1124/pr.56.4.3.
G. Powis, G. L. Kirkpatrick, Mol. Cancer Ther. 2004, 3, 647-654. https://doi.org/10.1158/1535-7163.647.3.5.
D. G. Nagle, Y. D. Zhou, Curr. Drug Targets 2006, 7, 355-369. doi: 10.2174/138945006776054979.
K. Yamaji, H. Ishimoto, N. Usui, S. Mori, Mycorrhiza 2005, 15, 17-23. DOI: 10.1007/s00572-003-0287-9.
H. L. L. Nang, C. Y. May, M. A. Ngan, C. C. Hock, Am. J. Environ. Sci. 2007, 3, 54-59. https://doi.org/10.3844/ajessp.2007.54.59.
K. W. Wahle, I. Brown, D. Rotondo, S. D. Heys, Adv. Exp. Med. Biol. 2010, 698, 36-51. DOI: 10.1007/978-1-4419-7347-4 4.
B. Awan, Int. Res. J. Pharm. 2013, 4, 186-188. DOI:10.7897/2230-8407.04339.
V. L. Singleton, J. A. Rossi, Am. J. Enol. Vitic. 1965, 16, 144-158.
S. Mamta, Mehrotra, V. Kirar Amitabh, P. Vats, S. P. Nandi, P. S. Negi, K. Mishra, Indian J. Exp. Biol. 2015, 53, 36-43. http://nopr.niscpr.res.in/handle/123456789/3027.
S. T. Chang, J. H. Wu, S. Y. Wang, P. L. Kang, N. S. Yang, L. F. Shyur, J. Agric. Food Chem. 2001, 49, 3420-3424 ;
T. C. Lin, K. H. Yu, C. M. Yang, C. C. Lin, Biol. Pharm. Bull. 2003, 26, 1331-1335. DOI: 10.1248/bpb.26.1331.
K. Thaipong, U. Boonprakoba, K. Crosbyb, L. Cisneros-Zevallosc, D. Hawkins Byrnec, J. Food Compos. Anal. 2006, 19, 669-675. DOI:10.1016/j.jfca.2006.01.003.
P. Jayanthi, P. Lalitha, Int. J. Pharm. Pharmaceut. Sci. 2011, 3 (3), 2011.
U. Golla, S. S. R. Bhimathati, The Scientific World Journal 2014, 2014, 1-8. doi: 10.1155/2014/215084.
C. Perez, M. Pauli, P. Bazerque, Arch. Biol. Med. Exp. 1990, 15, 113-115.
Y. T. Zheng, W. L. Chan, P. Chan, H. Huang, S. C. Tamm, FEBS Lett. 2001, 496, 139-142. DOI: 10.1016/s0014-5793(01)02391-2.
N. Kumar, R. C. Meena, A. Chakrabarti, Indian J. Microbiol. 2001, 512, 06-211. DOI: 10.1007/s12088-011-0132-3.
J. S. Leeuwen, N. P. E. Vermeulen, J. Vos, Curr. Drug Metab. 2012, 13, 1464-1475. DOI: 10.2174/138920012803762783.