Mitochondrial DNA quantification correlates with the developmental potential of human euploid blastocysts but not with that of mosaic blastocysts.
Blastocyst
Embryo viability
Mitochondrial genome
Mitochondrion
Preimplantation genetic testing
mtDNA quantification
Journal
BMC pregnancy and childbirth
ISSN: 1471-2393
Titre abrégé: BMC Pregnancy Childbirth
Pays: England
ID NLM: 100967799
Informations de publication
Date de publication:
15 Jun 2023
15 Jun 2023
Historique:
received:
25
03
2023
accepted:
05
06
2023
medline:
19
6
2023
pubmed:
16
6
2023
entrez:
15
6
2023
Statut:
epublish
Résumé
We aimed to study the association between adjusted mtDNA levels in human trophectoderm biopsy samples and the developmental potential of euploid and mosaic blastocysts. We analyzed relative mtDNA levels in 2,814 blastocysts obtained from 576 couples undergoing preimplantation genetic testing for aneuploidy from June 2018 to June 2021. All patients underwent in vitro fertilization in a single clinic; the study was blinded-mtDNA content was unknown at the time of single embryo transfer. The fate of the euploid or mosaic embryos transferred was compared with mtDNA levels. Euploid embryos had lower mtDNA than aneuploid and mosaic embryos. Embryos biopsied on Day 5 had higher mtDNA than those biopsied on Day 6. No difference was detected in mtDNA scores between embryos derived from oocytes of different maternal ages. Linear mixed model suggested that blastulation rate was associated with mtDNA score. Moreover, the specific next-generation sequencing platform used have a significant effect on the observed mtDNA content. Euploid embryos with higher mtDNA content presented significantly higher miscarriage rates and lower live birth rates, while no significant difference was observed in the mosaic cohort. Our results will aid in improving methods for analyzing the association between mtDNA level and blastocyst viability.
Identifiants
pubmed: 37322435
doi: 10.1186/s12884-023-05760-w
pii: 10.1186/s12884-023-05760-w
pmc: PMC10273751
doi:
Substances chimiques
DNA, Mitochondrial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
447Subventions
Organisme : Anhui Provincial Key R&D Program
ID : 201904a07020046
Organisme : National Natural Science Foundation of China
ID : U20A20350
Organisme : National Natural Science Foundation of China
ID : 82071613
Informations de copyright
© 2023. The Author(s).
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