The Protective Effect of Oxitard on Sperm Function and Antioxidant Status in Rats Exposed to Swimming Stress.
activity
albino rats
male fertility
mechanism
oxitard
shielding
spermatozoa
swimming stress
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
accepted:
13
06
2023
medline:
16
6
2023
pubmed:
16
6
2023
entrez:
16
6
2023
Statut:
epublish
Résumé
Infertility is a significant public health issue, but its impact on quality of life and treatment efficacy is limited. Modern medicine lacks safe and effective drugs for male infertility, while traditional medicine has explored herbal extracts like Oxitard, which contains multiple extracts and oils. This study aimed to investigate the effects of Oxitard on male rats exposed to swimming (SW) stress. Albino rats weighing 220-250 g were divided into five groups: control, SW stress, and SW treated with Oxitard at low, medium, and high doses of 250, 500, and 750 mg/kg/day, respectively. The rats were subjected to SW stress for 15 days and then assessed for body weight, reproductive organ weight, testosterone, antioxidant status, sperm function, and histological changes in the testes, seminal vesicles, and vas deferens. The results showed that SW stress significantly reduced body weight, seminal vesicle weight, testosterone levels, superoxide dismutase (SOD), catalase (CAT), sperm count, sperm motility, sperm viability, and significantly increased malondialdehyde (MDA) levels. The testes of the SW-stress group rats also showed a significant decrease in spermatogenesis and the number of seminiferous tubules containing sperm. In contrast, treatment with Oxitard, especially at the highest dose, demonstrated potent free radical scavenging activity, recovering antioxidant status, and sperm function. SW stress led to decreased sperm function, antioxidant status, and increased lipid peroxidation (LPO) in male rats. Oxitard treatment, particularly in high doses, showed a potential role as a free radical scavenger in treating oxidative stress (OS)-associated male infertility. Further studies are needed to investigate the individual components of Oxitard and conduct clinical trials in human subjects.
Sections du résumé
BACKGROUND
BACKGROUND
Infertility is a significant public health issue, but its impact on quality of life and treatment efficacy is limited. Modern medicine lacks safe and effective drugs for male infertility, while traditional medicine has explored herbal extracts like Oxitard, which contains multiple extracts and oils. This study aimed to investigate the effects of Oxitard on male rats exposed to swimming (SW) stress.
METHODS
METHODS
Albino rats weighing 220-250 g were divided into five groups: control, SW stress, and SW treated with Oxitard at low, medium, and high doses of 250, 500, and 750 mg/kg/day, respectively. The rats were subjected to SW stress for 15 days and then assessed for body weight, reproductive organ weight, testosterone, antioxidant status, sperm function, and histological changes in the testes, seminal vesicles, and vas deferens.
RESULTS
RESULTS
The results showed that SW stress significantly reduced body weight, seminal vesicle weight, testosterone levels, superoxide dismutase (SOD), catalase (CAT), sperm count, sperm motility, sperm viability, and significantly increased malondialdehyde (MDA) levels. The testes of the SW-stress group rats also showed a significant decrease in spermatogenesis and the number of seminiferous tubules containing sperm. In contrast, treatment with Oxitard, especially at the highest dose, demonstrated potent free radical scavenging activity, recovering antioxidant status, and sperm function.
CONCLUSION
CONCLUSIONS
SW stress led to decreased sperm function, antioxidant status, and increased lipid peroxidation (LPO) in male rats. Oxitard treatment, particularly in high doses, showed a potential role as a free radical scavenger in treating oxidative stress (OS)-associated male infertility. Further studies are needed to investigate the individual components of Oxitard and conduct clinical trials in human subjects.
Identifiants
pubmed: 37325690
doi: 10.7759/cureus.40381
pmc: PMC10264260
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e40381Informations de copyright
Copyright © 2023, Prashad et al.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Nat Protoc. 2010 Jan;5(1):51-66
pubmed: 20057381
J Sex Med. 2013 Oct;10(10):2443-54
pubmed: 23841462
Oxid Med Cell Longev. 2017;2017:8416763
pubmed: 28819546
Reprod Biol Endocrinol. 2018 Sep 11;16(1):87
pubmed: 30205828
Zhonghua Nan Ke Xue. 2010 Oct;16(10):867-71
pubmed: 21243747
Integr Med Res. 2013 Jun;2(2):56-61
pubmed: 28664055
Avicenna J Phytomed. 2023 Jan-Feb;13(1):7-17
pubmed: 36698735
Int J Prev Med. 2013 Jun;4(6):624-30
pubmed: 23930179
Molecules. 2018 Jul 18;23(7):
pubmed: 30022015
J Ethnopharmacol. 2004 Sep;94(1):135-41
pubmed: 15261974
Indian J Urol. 2011 Jan;27(1):41-8
pubmed: 21716889
Front Physiol. 2020 Jul 02;11:694
pubmed: 32714204
Am J Pathol. 2018 Apr;188(4):904-915
pubmed: 29378173
Antioxidants (Basel). 2022 Apr 29;11(5):
pubmed: 35624745
Endocrine. 2000 Aug;13(1):123-9
pubmed: 11051056
J Ayurveda Integr Med. 2017 Jul - Sep;8(3):169-176
pubmed: 28693955
Animals (Basel). 2019 Sep 29;9(10):
pubmed: 31569470
Front Psychiatry. 2015 May 04;6:61
pubmed: 25999864
Phytother Res. 2004 Jan;18(1):54-60
pubmed: 14750202
Acta Cir Bras. 2023 Apr 21;38:e381423
pubmed: 37098926
J Ethnopharmacol. 2001 Jan;74(1):1-6
pubmed: 11137343
Phytother Res. 2016 Sep;30(9):1392-403
pubmed: 27196869
J Hum Reprod Sci. 2019 Jan-Mar;12(1):4-18
pubmed: 31007461
Braz J Psychiatry. 2013;35 Suppl 2:S101-11
pubmed: 24271222
Nat Rev Urol. 2017 Aug;14(8):470-485
pubmed: 28508879
Indian J Exp Biol. 1997 Mar;35(3):236-9
pubmed: 9332168
J Hum Reprod Sci. 2014 Jan;7(1):41-6
pubmed: 24829530
Biomolecules. 2020 Jan 30;10(2):
pubmed: 32019140
Hum Exp Toxicol. 2019 Apr;38(4):409-418
pubmed: 30526067
Asian J Androl. 2013 Nov;15(6):790-4
pubmed: 23912311
Trends Pharmacol Sci. 2017 Jul;38(7):592-607
pubmed: 28551354
J Androl. 2009 Jan-Feb;30(1):75-86
pubmed: 18723472
Reprod Biol Endocrinol. 2020 Feb 17;18(1):10
pubmed: 32066450
Arab J Urol. 2013 Mar;11(1):101-5
pubmed: 26579254
World J Mens Health. 2014 Apr;32(1):1-17
pubmed: 24872947
Front Physiol. 2016 Nov 07;7:486
pubmed: 27872595
Life Sci. 2020 Jun 1;250:117545
pubmed: 32173313
J Oral Biol Craniofac Res. 2020 Oct-Dec;10(4):552-568
pubmed: 32939334
Arab J Urol. 2017 Dec 08;16(1):35-43
pubmed: 29713534
Phytother Res. 2009 Sep;23(9):1309-15
pubmed: 19172666
J Clin Exp Dent. 2014 Jul 01;6(3):e265-70
pubmed: 25136428
Hum Reprod Update. 2008 Jul-Aug;14(4):345-57
pubmed: 18535004
Endocr Rev. 2001 Jun;22(3):342-88
pubmed: 11399748
Evid Based Complement Alternat Med. 2018 Apr 29;2018:8521782
pubmed: 29853975
Int J Biomed Sci. 2008 Jun;4(2):89-96
pubmed: 23675073
Molecules. 2021 Oct 22;26(21):
pubmed: 34770801
J Pharm Pharmacol. 2015 Jul;67(7):879-99
pubmed: 25828061
J Clin Diagn Res. 2014 Jul;8(7):HC05-8
pubmed: 25177581
J Sex Marital Ther. 2014;40(5):379-95
pubmed: 24313631
Braz J Med Biol Res. 2003 May;36(5):677-81
pubmed: 12715088
Semin Reprod Med. 2009 Mar;27(2):115-23
pubmed: 19247913
World J Mens Health. 2022 Apr;40(2):228-242
pubmed: 34666422
J Pharm Bioallied Sci. 2015 Aug;7(Suppl 2):S331-3
pubmed: 26538872
Int J Environ Res Public Health. 2019 Feb 22;16(4):
pubmed: 30813224
Reprod Biol Endocrinol. 2013 Jul 16;11:66
pubmed: 23870423
Int J Reprod Biomed. 2016 Dec;14(12):729-736
pubmed: 28066832
Int J Biol Sci. 2006;2(1):23-9
pubmed: 16680200
Anim Biosci. 2021 Aug;34(8):1253-1270
pubmed: 33902175
Front Behav Neurosci. 2021 Feb 16;15:655214
pubmed: 33664658
Arch Anim Breed. 2023 Mar 02;66(1):113-120
pubmed: 36935777
Bioinformation. 2021 Feb 28;17(2):295-305
pubmed: 34234388
Antioxidants (Basel). 2022 Apr 22;11(5):
pubmed: 35624683
J Reprod Infertil. 2012 Apr;13(2):71-80
pubmed: 23926528
Asian-Australas J Anim Sci. 2017 May;30(5):622-637
pubmed: 26954196
Front Vet Sci. 2021 Apr 16;8:601794
pubmed: 33937366
BMC Biochem. 2018 Aug 3;19(1):7
pubmed: 30075706
Curr Issues Mol Biol. 2023 Apr 07;45(4):3255-3267
pubmed: 37185736
J Clin Endocrinol Metab. 2006 Oct;91(10):4154-63
pubmed: 16895947
Reprod Biomed Online. 2019 Aug;39(2):269-279
pubmed: 31160241
Biochem J. 1940 Nov;34(10-11):1464-71
pubmed: 16747278
J Cancer Res Ther. 2015 Apr-Jun;11(2):291-4
pubmed: 26148587
Biol Reprod. 2003 Jul;69(1):242-7
pubmed: 12620935
Pharmacol Res. 2016 Sep;111:180-200
pubmed: 27320046
Eur J Pharmacol. 1978 Feb 15;47(4):379-91
pubmed: 204499
Bio Protoc. 2017 Aug 20;7(16):e2505
pubmed: 34541169
Iran J Reprod Med. 2014 Feb;12(2):131-8
pubmed: 24799871
Endocrinology. 1992 Apr;130(4):1780-8
pubmed: 1312425
Vet Med Int. 2010 Sep 07;2010:
pubmed: 20871827
J Hum Reprod Sci. 2015 Oct-Dec;8(4):191-6
pubmed: 26752853
Oxid Med Cell Longev. 2015;2015:804794
pubmed: 25874024
Int J Mol Sci. 2019 Sep 10;20(18):
pubmed: 31510090
Pharmacogn Rev. 2010 Jul;4(8):118-26
pubmed: 22228951
Endocrinology. 1997 Nov;138(11):4806-11
pubmed: 9348209