Fatty acid fingerprints in bronchoalveolar lavage fluid and its extracellular vesicles reflect equine asthma severity.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 06 2023
17 06 2023
Historique:
received:
14
12
2022
accepted:
08
06
2023
medline:
19
6
2023
pubmed:
18
6
2023
entrez:
17
6
2023
Statut:
epublish
Résumé
Equine asthma (EA) is an inflammatory disease of the lower airways driven by mediators released from cells. Extracellular vesicles (EVs) are vehicles for lipid mediators, which possess either pro-inflammatory or dual anti-inflammatory and pro-resolving functions. In this study, we investigated how the respiratory fatty acid (FA) profile reflects airway inflammatory status. The FA composition of bronchoalveolar lavage fluid (BALF), BALF supernatant, and bronchoalveolar EVs of healthy horses (n = 15) and horses with mild/moderate EA (n = 10) or severe EA (SEA, n = 5) was determined with gas chromatography and mass spectrometry. The FA profiles distinguished samples with different diagnoses in all sample types, yet they were insufficient to predict the health status of uncategorized samples. Different individual FAs were responsible for the discrimination of the diagnoses in different sample types. Particularly, in the EVs of SEA horses the proportions of palmitic acid (16:0) decreased and those of eicosapentaenoic acid (20:5n-3) increased, and all sample types of asthmatic horses had elevated dihomo-γ-linolenic acid (20:3n-6) proportions. The results suggest simultaneous pro-inflammatory and resolving actions of FAs and a potential role for EVs as vehicles for lipid mediators in asthma pathogenesis. EV lipid manifestations of EA can offer translational targets to study asthma pathophysiology and treatment options.
Identifiants
pubmed: 37330591
doi: 10.1038/s41598-023-36697-x
pii: 10.1038/s41598-023-36697-x
pmc: PMC10276833
doi:
Substances chimiques
Fatty Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9821Informations de copyright
© 2023. The Author(s).
Références
Nature. 2014 Jun 5;510(7503):92-101
pubmed: 24899309
Inflammation. 2023 May 4;:
pubmed: 37140681
Am J Vet Res. 2019 Mar;80(3):300-305
pubmed: 30801214
Prostaglandins Leukot Essent Fatty Acids. 1988;32(3):165-74
pubmed: 2901111
Equine Vet J. 2004 Nov;36(7):576-82
pubmed: 15581321
J Vet Intern Med. 2016 Mar-Apr;30(2):503-15
pubmed: 26806374
Biochim Biophys Acta. 2013 Jul;1831(7):1302-9
pubmed: 24046871
J Neurooncol. 2013 May;113(1):1-11
pubmed: 23456661
J Allergy Clin Immunol. 2014 May;133(5):1255-64
pubmed: 24613565
Am J Vet Res. 2010 Apr;71(4):468-75
pubmed: 20367056
PLoS Biol. 2020 Jul 14;18(7):e3000411
pubmed: 32663221
Mol Immunol. 2015 Jul;66(1):97-105
pubmed: 25547716
Medicina (Kaunas). 2019 Jun 18;55(6):
pubmed: 31216723
Eur J Pharmacol. 2016 Aug 15;785:144-155
pubmed: 26546247
Nat Rev Immunol. 2014 Mar;14(3):195-208
pubmed: 24566916
Biochim Biophys Acta. 2013 Mar;1831(3):612-25
pubmed: 23026158
Int J Mol Sci. 2022 May 17;23(10):
pubmed: 35628422
Br J Nutr. 2006 Apr;95(4):752-61
pubmed: 16571155
Am J Vet Res. 2022 Jul 11;83(9):
pubmed: 35895773
J Vet Intern Med. 2015 Jan;29(1):299-306
pubmed: 25307169
Sci Rep. 2017 Feb 01;7:40555
pubmed: 28145528
Am J Respir Crit Care Med. 1999 Jun;159(6):1803-9
pubmed: 10351922
Biochem Pharmacol. 2020 Sep;179:113925
pubmed: 32217103
Am J Respir Cell Mol Biol. 2001 Dec;25(6):725-31
pubmed: 11726398
Equine Vet J. 2003 Jul;35(5):491-5
pubmed: 12875328
J Lipid Res. 2018 Aug;59(8):1325-1340
pubmed: 29853528
Adv Drug Deliv Rev. 2020;159:308-321
pubmed: 32151658
Thorax. 2004 Feb;59(2):105-10
pubmed: 14760147
Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Aug;1864(8):1168-1182
pubmed: 30980920
Biomed Pharmacother. 2002 Oct;56(8):388-96
pubmed: 12442911
Front Vet Sci. 2020 Jul 30;7:450
pubmed: 32903600
Sci Rep. 2018 Jul 9;8(1):10340
pubmed: 29985427
Front Immunol. 2020 Dec 09;11:580598
pubmed: 33362766
Allergy Asthma Immunol Res. 2014 Jan;6(1):61-5
pubmed: 24404395
J Vet Intern Med. 2008 Nov-Dec;22(6):1452-5
pubmed: 18761600
J Appl Physiol (1985). 2000 Oct;89(4):1283-92
pubmed: 11007560
Matrix Biol. 2019 Jan;75-76:201-219
pubmed: 29066152
Clin Exp Allergy. 2003 May;33(5):676-83
pubmed: 12752598
Adv Drug Deliv Rev. 2020;159:133-169
pubmed: 32628989
Arthritis Res Ther. 2019 May 22;21(1):124
pubmed: 31118103
Arch Dis Child Fetal Neonatal Ed. 2019 Jul;104(4):F443-F451
pubmed: 30552091
Cells. 2020 Jul 02;9(7):
pubmed: 32630649
J Vet Intern Med. 2018 Sep;32(5):1754-1762
pubmed: 30222207
Prostaglandins Other Lipid Mediat. 2022 Feb;158:106609
pubmed: 34954219
Nat Immunol. 2001 Jul;2(7):612-9
pubmed: 11429545
Front Immunol. 2018 Apr 12;9:771
pubmed: 29706969
Nature. 1968 Jan 6;217(5123):69
pubmed: 4383955
Nat Commun. 2019 Jun 21;10(1):2752
pubmed: 31227693
Front Vet Sci. 2022 Jun 21;9:894189
pubmed: 35799843
Front Vet Sci. 2017 Sep 28;4:159
pubmed: 29034248
Pharmacology. 2010;85(6):336-49
pubmed: 20516735
Metabolomics. 2021 Apr 17;17(5):41
pubmed: 33866431
Tumour Biol. 2015 Jul;36(7):5687-98
pubmed: 25702090