Developmental-stage specific single-cell human embryo dissociation.
Developmental biology
Single Cell
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 Jun 2023
17 Jun 2023
Historique:
received:
26
09
2022
revised:
24
02
2023
accepted:
17
05
2023
medline:
18
6
2023
pubmed:
18
6
2023
entrez:
18
6
2023
Statut:
aheadofprint
Résumé
Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection. We then detail embryo dissociation followed by steps to pick, wash, and dispense cells in plates.
Identifiants
pubmed: 37330906
pii: S2666-1667(23)00330-1
doi: 10.1016/j.xpro.2023.102363
pmc: PMC10300299
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102363Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Curr Opin Obstet Gynecol. 1999 Jun;11(3):307-11
pubmed: 10369209
Hum Reprod. 2011 Jun;26(6):1270-83
pubmed: 21502182
Hum Reprod. 2016 Apr;31(4):685-6
pubmed: 26908842
Cell Stem Cell. 2021 Sep 2;28(9):1625-1640.e6
pubmed: 34004179