Detection of Specific RNA Targets by Multimerization.
coronavirus SARS-CoV-2
isothermal amplification
molecular diagnostics
multimerization
nearby primers
ribonucleic acids (RNA)
Journal
Biochemistry. Biokhimiia
ISSN: 1608-3040
Titre abrégé: Biochemistry (Mosc)
Pays: United States
ID NLM: 0376536
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
medline:
20
6
2023
pubmed:
19
6
2023
entrez:
18
6
2023
Statut:
ppublish
Résumé
Detection of specific RNA targets via amplification-mediated techniques is widely used in fundamental studies and medicine due to essential role of RNA in transfer of genetic information and development of diseases. Here, we report on an approach for detection of RNA targets based on the particular type of isothermal amplification, namely, reaction of nucleic acid multimerization. The proposed technique requires only a single DNA polymerase possessing reverse transcriptase, DNA-dependent DNA polymerase, and strand-displacement activities. Reaction conditions that lead to efficient detection of the target RNAs through multimerization mechanism were determined. The approach was verified by using genetic material of the SARS-CoV-2 coronavirus as a model viral RNA. Reaction of multimerization allowed to differentiate the SARS-CoV-2 RNA-positive samples from the SARS-CoV-2 negative samples with high reliability. The proposed technique allows detection of RNA even in the samples, which were subjected to multiple freezing-thawing cycles.
Identifiants
pubmed: 37331713
pii: BCM88050832
doi: 10.1134/S0006297923050103
pmc: PMC10193353
doi:
Substances chimiques
RNA, Viral
0
DNA-Directed DNA Polymerase
EC 2.7.7.7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
679-686Références
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