Comparative B cell and antibody responses induced by adenoviral vectored and mRNA vaccines against COVID-19.

Journal

medRxiv : the preprint server for health sciences
Titre abrégé: medRxiv
Pays: United States
ID NLM: 101767986

Informations de publication

Date de publication:
05 Jun 2023
Historique:
pubmed: 19 6 2023
medline: 19 6 2023
entrez: 19 6 2023
Statut: epublish

Résumé

Both vector and mRNA vaccines were an important part of the response to the COVID-19 pandemic and may be required in future outbreaks and pandemics. However, adenoviral vectored (AdV) vaccines may be less immunogenic than mRNA vaccines against SARS-CoV-2. We assessed anti-spike and anti-vector immunity among infection-naïve Health Care Workers (HCW) following two doses of AdV (AZD1222) versus mRNA (BNT162b2) vaccine. 183 AdV and 274 mRNA vaccinees enrolled between April and October 2021. Median ages were 42 and 39 years, respectively. Blood was collected at least once, 10-48 days after vaccine dose 2. Surrogate virus neutralization test (sVNT) and spike binding antibody titres were a median of 4.2 and 2.2 times lower, respectively, for AdV compared to mRNA vaccinees (p<0.001). Median percentages of memory B cells that recognized fluorescent-tagged spike and RBD were 2.9 and 8.3 times lower, respectively for AdV compared to mRNA vaccinees. Titres of IgG reactive with human Adenovirus type 5 hexon protein rose a median of 2.2-fold after AdV vaccination but were not correlated with anti-spike antibody titres. Together the results show that mRNA induced substantially more sVNT antibody than AdV vaccine due to greater B cell expansion and targeting of the RBD. Pre-existing AdV vector cross-reactive antibodies were boosted following AdV vaccination but had no detectable effect on immunogenicity.

Identifiants

DOI: 10.1101/2023.06.02.23290871 PMID: 37333329 PMC: PMC10275006
pubmed: 37333329
doi: 10.1101/2023.06.02.23290871
pmc: PMC10275006
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI141534
Pays : United States

Déclaration de conflit d'intérêts

AF reports research funding from Sanofi. HM reports research funding from bioCSL, Pfizer, and GlaxoSmithKline. PW reports research funding from GlaxoSmithKline, Krystal Australia Pty Ltd, Vertex Pharmaceuticals, and Sanofi. SGS reports honoraria from Pfizer and CSL Seqirus.

Auteurs

Yi Liu (Y)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Stephany Sánchez-Ovando (S)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Louise Carolan (L)

WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Leslie Dowson (L)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Arseniy Khvorov (A)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Jessica Hadiprodjo (J)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Yeu Yang Tseng (YY)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Catherine Delahunty (C)

Immune Health Program, Hunter Medical Research Institute and School of Medicine and Public Health, University of Newcastle, Newcastle, Australia.

Ameneh Khatami (A)

The Children's Hospital at Westmead; Sydney Children's Hospital Network; National Centre for Immunisation Research and Surveillance, Sydney, Australia.
The University of Sydney; and National Centre for Immunisation Research and Surveillance, Sydney, Australia.

Marion Macnish (M)

Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.

Sonia Dougherty (S)

Queensland Children's Hospital, Children's Health Queensland Hospital and Health Service; and University of Queensland, Brisbane, Australia.

Michelle Hagenauer (M)

Alfred Health and Monash University, Melbourne, Australia.

Kathryn E Riley (KE)

Adelaide Medical School and Robinson Research Institute, The University of Adelaide, Adelaide, Australia.
Division of Paediatric Medicine, Women's and Children's Health Network, Adelaide, Australia.

Ajay Jadhav (A)

The Children's Hospital at Westmead; Sydney Children's Hospital Network; National Centre for Immunisation Research and Surveillance, Sydney, Australia.

Joanne Harvey (J)

Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.

Marti Kaiser (M)

Alfred Health and Monash University, Melbourne, Australia.

Suja Mathew (S)

Adelaide Medical School and Robinson Research Institute, The University of Adelaide, Adelaide, Australia.
Division of Paediatric Medicine, Women's and Children's Health Network, Adelaide, Australia.

David Hodgson (D)

Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK.

Vivian Leung (V)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Kanta Subbarao (K)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Allen C Cheng (AC)

Alfred Health and Monash University, Melbourne, Australia.

Kristine Macartney (K)

The Children's Hospital at Westmead; Sydney Children's Hospital Network; National Centre for Immunisation Research and Surveillance, Sydney, Australia.
The University of Sydney; and National Centre for Immunisation Research and Surveillance, Sydney, Australia.

Archana Koirala (A)

The Children's Hospital at Westmead; Sydney Children's Hospital Network; National Centre for Immunisation Research and Surveillance, Sydney, Australia.
The University of Sydney; and National Centre for Immunisation Research and Surveillance, Sydney, Australia.

Helen Marshall (H)

Adelaide Medical School and Robinson Research Institute, The University of Adelaide, Adelaide, Australia.
Division of Paediatric Medicine, Women's and Children's Health Network, Adelaide, Australia.

Julia Clark (J)

Queensland Children's Hospital, Children's Health Queensland Hospital and Health Service; and University of Queensland, Brisbane, Australia.

Christopher C Blyth (CC)

Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.
School of Medicine, University of Western Australia; Perth Children's Hospital; and Department of Microbiology, PathWest Laboratory Medicine, QEII medical centre, Perth, Australia.

Peter Wark (P)

Immune Health Program, Hunter Medical Research Institute and School of Medicine and Public Health, University of Newcastle, Newcastle, Australia.

Adam J Kucharski (AJ)

Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, UK.

Sheena G Sullivan (SG)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Department of Epidemiology, University of California, Los Angeles, USA.

Annette Fox (A)

Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Classifications MeSH