Effects of linker length on phase separation: lessons from the Rubisco-EPYC1 system of the algal pyrenoid.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
11 Jun 2023
Historique:
pubmed: 19 6 2023
medline: 19 6 2023
entrez: 19 6 2023
Statut: epublish

Résumé

Biomolecular condensates are membraneless organelles formed via phase separation of macromolecules, typically consisting of bond-forming "stickers" connected by flexible "linkers". Linkers have diverse roles, such as occupying space and facilitating interactions. To understand how linker length relative to other lengths affects condensation, we focus on the pyrenoid, which enhances photosynthesis in green algae. Specifically, we apply coarse-grained simulations and analytical theory to the pyrenoid proteins of

Identifiants

DOI: 10.1101/2023.06.11.544494 PMID: 37333342 PMC: PMC10274861
pubmed: 37333342
doi: 10.1101/2023.06.11.544494
pmc: PMC10274861
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM140032
Pays : United States

Déclaration de conflit d'intérêts

Competing interests The authors declare no competing interests.

Auteurs

Trevor GrandPre (T)

Department of Physics, Princeton University, Princeton, NJ 08544, USA.
Center for the Physics of Biological Function, Princeton University, Princeton, NJ 08544, USA.
Princeton Center for Theoretical Science, Princeton University, Princeton, NJ 08544, USA.

Yaojun Zhang (Y)

Center for the Physics of Biological Function, Princeton University, Princeton, NJ 08544, USA.
Department of Physics and Astronomy, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.

Andrew G T Pyo (AGT)

Department of Physics, Princeton University, Princeton, NJ 08544, USA.
Center for the Physics of Biological Function, Princeton University, Princeton, NJ 08544, USA.

Benjamin Weiner (B)

Department of Physics, Princeton University, Princeton, NJ 08544, USA.
Center for the Physics of Biological Function, Princeton University, Princeton, NJ 08544, USA.

Je-Luen Li (JL)

D. E. Shaw Research, LLC, New York, NY 10036, USA.

Martin C Jonikas (MC)

Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA.
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

Ned S Wingreen (NS)

Center for the Physics of Biological Function, Princeton University, Princeton, NJ 08544, USA.
Princeton Center for Theoretical Science, Princeton University, Princeton, NJ 08544, USA.
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.

Classifications MeSH