Development of RT-qPCR for quantification of human enterovirus D68
Detection
Enterovirus D68
Human Rhinovirus 87
Quantification
Real-time PCR
Respiratory infection
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay
Journal
MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829
Informations de publication
Date de publication:
2023
2023
Historique:
received:
10
03
2023
accepted:
26
05
2023
medline:
19
6
2023
pubmed:
19
6
2023
entrez:
19
6
2023
Statut:
epublish
Résumé
The common cold is the most frequent viral infectious disease of the upper respiratory tract with different intensities based on the serotype and the characteristics of the virus. Numerous human rhinoviruses have been identified and classified. Human rhinovirus 87 (HRV87), also known as enterovirus D68 (EV-D68), is one of the common viruses causing respiratory infections. In this study, a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay was developed, optimized, and validated for the detection of EV-D68. Method development also covers specificity, sensitivity, efficiency, and inter-and-intra-assay variations. Overall, this one-step qPCR assay will permit quantitative assessments of human enterovirus D68 RNA.•Enterovirus D68 is a reemerging viral agent causing respiratory infection.•RT-qPCR assay developed for detection of human enterovirus D68.•In this article validation to secure reproducibility is done according to MIQE guidelines.
Identifiants
pubmed: 37333509
doi: 10.1016/j.mex.2023.102234
pii: S2215-0161(23)00231-5
pmc: PMC10272510
doi:
Types de publication
Journal Article
Langues
eng
Pagination
102234Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
PLoS Negl Trop Dis. 2022 Feb 8;16(2):e0010073
pubmed: 35134062
Clin Chem. 2009 Apr;55(4):611-22
pubmed: 19246619
J Clin Microbiol. 2015 Aug;53(8):2641-7
pubmed: 26063859
J Mol Diagn. 2010 Jan;12(1):102-8
pubmed: 19948820
Intervirology. 2002;45(3):136-41
pubmed: 12403917
J Clin Microbiol. 2008 Feb;46(2):533-9
pubmed: 18057136
J Infect Dis. 1987 Jul;156(1):43-9
pubmed: 3036962
Elife. 2021 Jul 01;10:
pubmed: 34196272
Virology. 1991 Feb;180(2):814-7
pubmed: 1846502
Clin Microbiol Rev. 2013 Jan;26(1):135-62
pubmed: 23297263
PLoS One. 2017 Jun 2;12(6):e0178408
pubmed: 28575086
J Clin Microbiol. 2002 Nov;40(11):4218-23
pubmed: 12409401
Viruses. 2019 May 29;11(6):
pubmed: 31146373
J Clin Microbiol. 1998 Jul;36(7):2081-3
pubmed: 9650967
Cell Mol Life Sci. 1998 Dec;54(12):1330-49
pubmed: 9893709
J Clin Microbiol. 2007 Jul;45(7):2126-9
pubmed: 17475758