The association of intraleucocytic malaria pigment and disease severity in Papua New Guinean children with severe P. falciparum malaria.
Plasmodium falciparum
Papua New Guinea
children
pigment-containing monocytes
pigment-containing neutrophils
severe malaria
Journal
Transactions of the Royal Society of Tropical Medicine and Hygiene
ISSN: 1878-3503
Titre abrégé: Trans R Soc Trop Med Hyg
Pays: England
ID NLM: 7506129
Informations de publication
Date de publication:
03 Nov 2023
03 Nov 2023
Historique:
received:
22
01
2023
revised:
10
04
2023
accepted:
19
05
2023
pmc-release:
19
06
2024
medline:
9
11
2023
pubmed:
19
6
2023
entrez:
19
6
2023
Statut:
ppublish
Résumé
Plasmodium falciparum pigment-containing leucocytes (PCLs) are associated with adverse clinical manifestations of severe malaria in African children. However, limited data exist on the association of PCLs in settings outside of Africa. Thin films on peripheral blood slides obtained from children ages 6 months-10 y with severe malaria were examined for PCLs. The intraleucocytic pigment data were correlated with clinical phenotypic data such as severe anaemia, metabolic acidosis and coma to determine the association of PCLs with clinical phenotypes of severe malaria and outcome. Of the 169 children with severe P. falciparum malaria confirmed by microscopy, 76% (129/169) had PCLs. Compared with children without PCLs, the presence (adjusted odds ratio [AOR] 3.2 [95% confidence interval {CI} 1.5 to 6.9], p≤0.01) and quantity (AOR 1.0 [95% CI 1.0 to 1.1], p=0.04) of pigment-containing monocytes (PCMs) was significantly associated with severe anaemia, while the quantity of both PCMs (AOR 1.0 [95% CI 1.0 to 1.1], p≤0.01) and pigment-containing neutrophils (AOR 1.0 [95% CI 1.0 to 1.1], p=0.01) was significantly associated with metabolic acidosis. Plasma P. falciparum histidine-rich protein-2 level negatively correlated with the platelet count (r=-0.5, p≤0.01) in patients with PCLs and no PCLs. In Papua New Guinean children with severe P. falciparum malaria, the presence and quantity of PCLs are predictors of disease severity, severe anaemia and metabolic acidosis.
Sections du résumé
BACKGROUND
BACKGROUND
Plasmodium falciparum pigment-containing leucocytes (PCLs) are associated with adverse clinical manifestations of severe malaria in African children. However, limited data exist on the association of PCLs in settings outside of Africa.
METHODS
METHODS
Thin films on peripheral blood slides obtained from children ages 6 months-10 y with severe malaria were examined for PCLs. The intraleucocytic pigment data were correlated with clinical phenotypic data such as severe anaemia, metabolic acidosis and coma to determine the association of PCLs with clinical phenotypes of severe malaria and outcome.
RESULTS
RESULTS
Of the 169 children with severe P. falciparum malaria confirmed by microscopy, 76% (129/169) had PCLs. Compared with children without PCLs, the presence (adjusted odds ratio [AOR] 3.2 [95% confidence interval {CI} 1.5 to 6.9], p≤0.01) and quantity (AOR 1.0 [95% CI 1.0 to 1.1], p=0.04) of pigment-containing monocytes (PCMs) was significantly associated with severe anaemia, while the quantity of both PCMs (AOR 1.0 [95% CI 1.0 to 1.1], p≤0.01) and pigment-containing neutrophils (AOR 1.0 [95% CI 1.0 to 1.1], p=0.01) was significantly associated with metabolic acidosis. Plasma P. falciparum histidine-rich protein-2 level negatively correlated with the platelet count (r=-0.5, p≤0.01) in patients with PCLs and no PCLs.
CONCLUSIONS
CONCLUSIONS
In Papua New Guinean children with severe P. falciparum malaria, the presence and quantity of PCLs are predictors of disease severity, severe anaemia and metabolic acidosis.
Identifiants
pubmed: 37334767
pii: 7201932
doi: 10.1093/trstmh/trad037
pmc: PMC10629949
doi:
Substances chimiques
hemozoin
39404-00-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
797-803Subventions
Organisme : NIAID NIH HHS
ID : U19 AI089686
Pays : United States
Organisme : NIH HHS
ID : U19 AI129392-01
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene.
Références
PLoS Med. 2012;9(8):e1001297
pubmed: 22927801
Sci Transl Med. 2022 Jul 20;14(654):eabn5040
pubmed: 35857826
Trans R Soc Trop Med Hyg. 1998 Jan-Feb;92(1):54-6
pubmed: 9692152
Am J Trop Med Hyg. 2003 Sep;69(3):253-9
pubmed: 14628940
Trans R Soc Trop Med Hyg. 1994 Jan-Feb;88(1):67-73
pubmed: 8154008
QJM. 1996 Oct;89(10):779-88
pubmed: 8944234
Am J Trop Med Hyg. 2019 Apr;100(4):835-841
pubmed: 30793683
Malar J. 2015 May 28;14:219
pubmed: 26017395
N Engl J Med. 1995 May 25;332(21):1399-404
pubmed: 7723795
Trans R Soc Trop Med Hyg. 1999 May-Jun;93(3):283-6
pubmed: 10492760
Clin Infect Dis. 2011 Feb 15;52(4):440-6
pubmed: 21216895
Nat Commun. 2022 Nov 12;13(1):6882
pubmed: 36371433
Blood. 1996 Dec 15;88(12):4694-700
pubmed: 8977263
PLoS Med. 2008 Jun 17;5(6):e128
pubmed: 18563962
Am J Trop Med Hyg. 2011 Jul;85(1):50-4
pubmed: 21734123
PLoS Negl Trop Dis. 2012;6(12):e1972
pubmed: 23272266
Malar J. 2014 Apr 16;13:145
pubmed: 24739250
Trans R Soc Trop Med Hyg. 2000 Apr;94 Suppl 1:S1-90
pubmed: 11103309
Trans R Soc Trop Med Hyg. 1995 Nov-Dec;89(6):637-8
pubmed: 8594680
Trans R Soc Trop Med Hyg. 1995 Mar-Apr;89(2):200-4
pubmed: 7778149
J Infect Dis. 2009 Jan 1;199(1):142-50
pubmed: 19086817
PLoS One. 2011;6(12):e29203
pubmed: 22216212