Identification of common sequence motifs shared exclusively among selectively packed exosomal pathogenic microRNAs during rickettsial infections.
endothelial barrier dysfunction
exosome
fluidic AFM
microRNA
rickettsial infection
sequence motif
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
12
05
2023
received:
10
04
2023
accepted:
22
05
2023
medline:
17
8
2023
pubmed:
19
6
2023
entrez:
19
6
2023
Statut:
ppublish
Résumé
We previously reported that microRNA (miR)23a and miR30b are selectively sorted into exosomes derived from rickettsia-infected endothelial cells (R-ECExos). Yet, the mechanism remains unknown. Cases of spotted fever rickettsioses have been increasing, and infections with these bacteria cause life-threatening diseases by targeting brain and lung tissues. Therefore, the goal of the present study is to further dissect the molecular mechanism underlying R-ECExos-induced barrier dysfunction of normal recipient microvascular endothelial cells (MECs), depending on their exosomal RNA cargos. Infected ticks transmit the rickettsiae to human hosts following a bite and injections of the bacteria into the skin. In the present study, we demonstrate that treatment with R-ECExos, which were derived from spotted fever group R parkeri infected human dermal MECs, induced disruptions of the paracellular adherens junctional protein VE-cadherin, and breached the paracellular barrier function in recipient pulmonary MECs (PMECs) in an exosomal RNA-dependent manner. We did not detect different levels of miRs in parent dermal MECs following rickettsial infections. However, we demonstrated that the microvasculopathy-relevant miR23a-27a-24 cluster and miR30b are selectively enriched in R-ECExos. Bioinformatic analysis revealed that common sequence motifs are shared exclusively among the exosomal, selectively-enriched miR23a cluster and miR30b at different levels. Taken together, these data warrant further functional identification and characterization of a monopartition, bipartition, or tripartition among ACA, UCA, and CAG motifs that guide recognition of microvasculopathy-relevant miR23a-27a-24 and miR30b, and subsequently results in their selective enrichments in R-ECExos.
Substances chimiques
MicroRNAs
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1937-1948Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL142758
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI121012
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI137785
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI154211
Pays : United States
Organisme : NIAID NIH HHS
ID : R03 AI142406
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI144328
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG066060
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 Wiley Periodicals LLC.
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