Comparative characterization of Crimean-Congo hemorrhagic fever virus cell culture systems with application to propagation and titration methods.
BSR-T7/5
Cell culture
Cell line
Crimean-Congo hemorrhagic fever virus
HuH-7
Plaque assay
SW-13
TCID50
Titration
Vero E6
Journal
Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645
Informations de publication
Date de publication:
19 06 2023
19 06 2023
Historique:
received:
07
12
2022
accepted:
02
06
2023
medline:
21
6
2023
pubmed:
20
6
2023
entrez:
19
6
2023
Statut:
epublish
Résumé
Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) is a biosafety level 4 and World Health Organization top priority pathogen. Infection leads to an often fatal hemorrhagic fever disease in humans. The tick-borne virus is endemic in countries across Asia, Europe and Africa, with signs of spreading into new regions. Despite the severity of disease and the potential of CCHFV geographic expansion to cause widespread outbreaks, no approved vaccine or treatment is currently available. Critical for basic research and the development of diagnostics or medical countermeasures, CCHFV viral stocks are commonly produced in Vero E6 and SW-13 cell lines. While a variety of in-house methods are being used across different laboratories, there has been no clear, specific consensus on a standard, optimal system for CCHFV growth and titration. In this study, we perform a systematic, side-by-side characterization of Vero E6 and SW-13 cell lines concerning the replication kinetics of CCHFV under different culture conditions. SW-13 cells are typically cultured in a CO
Identifiants
pubmed: 37337294
doi: 10.1186/s12985-023-02089-w
pii: 10.1186/s12985-023-02089-w
pmc: PMC10280830
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
128Informations de copyright
© 2023. Crown.
Références
Vector Borne Zoonotic Dis. 2017 Oct;17(10):714-719
pubmed: 28836897
Heliyon. 2021 Oct 12;7(10):e08161
pubmed: 34703927
J Med Virol. 2008 Aug;80(8):1397-404
pubmed: 18551619
J Virol. 2015 Oct;89(20):10219-29
pubmed: 26223644
Virol J. 2008 Jan 15;5:7
pubmed: 18197964
J Virol. 2022 Feb 9;96(3):e0156821
pubmed: 34817199
Sci Rep. 2019 May 23;9(1):7755
pubmed: 31123310
PLoS One. 2017 Oct 5;12(10):e0186079
pubmed: 28982126
Ticks Tick Borne Dis. 2020 May;11(3):101403
pubmed: 32037097
Pharmazie. 2013 Oct;68(10):805-10
pubmed: 24273884
PLoS Negl Trop Dis. 2022 Mar 10;16(3):e0010285
pubmed: 35271569
Viruses. 2019 Mar 09;11(3):
pubmed: 30857305
Elife. 2022 Apr 19;11:
pubmed: 35437144
Antiviral Res. 2022 Nov;207:105401
pubmed: 36049554
J Virol. 2003 May;77(10):5997-6006
pubmed: 12719591
Sci Rep. 2021 Jan 27;11(1):2324
pubmed: 33504869
NPJ Vaccines. 2021 Mar 2;6(1):31
pubmed: 33654101
Sci Rep. 2020 Jul 23;10(1):12378
pubmed: 32704046
J Clin Invest. 2020 Dec 1;130(12):6429-6442
pubmed: 32853182
J Virol. 2002 Jul;76(14):7263-75
pubmed: 12072526
Emerg Infect Dis. 2015 Jan;21(1):23-31
pubmed: 25529825
Microorganisms. 2020 May 21;8(5):
pubmed: 32455700
Nat Microbiol. 2018 May;3(5):556-562
pubmed: 29632370
J Virol. 2011 Aug;85(15):7766-74
pubmed: 21632768
PLoS One. 2018 Aug 28;13(8):e0202997
pubmed: 30153293
Curr Protoc Microbiol. 2008 Nov;Appendix 4:Appendix 4E
pubmed: 19016439
PLoS Negl Trop Dis. 2020 Nov 23;14(11):e0008834
pubmed: 33226988
Nat Microbiol. 2021 Feb;6(2):187-195
pubmed: 33257849
PLoS One. 2016 Jun 07;11(6):e0156637
pubmed: 27272940
PLoS Negl Trop Dis. 2015 Mar 11;9(3):e0003579
pubmed: 25760444
Cell. 2021 Jun 24;184(13):3486-3501.e21
pubmed: 34077751
J Virol. 1999 Jan;73(1):251-9
pubmed: 9847328
Virol Sin. 2023 Feb;38(1):34-46
pubmed: 36075566
Elife. 2021 Jan 08;10:
pubmed: 33416494
J Infect Dis. 2017 Dec 12;216(11):1386-1397
pubmed: 28482001
Antiviral Res. 2017 Nov;147:91-99
pubmed: 29024765
Mol Ther. 2023 Feb 1;31(2):387-397
pubmed: 36184852
J Gen Virol. 2010 Jan;91(Pt 1):189-98
pubmed: 19812268
J Natl Cancer Inst. 1973 Aug;51(2):691-7
pubmed: 4765382
Viruses. 2018 Jul 20;10(7):
pubmed: 30036960
Elife. 2020 Oct 21;9:
pubmed: 33084573
J Cell Sci. 1986 Mar;81:65-103
pubmed: 3733899
Emerg Infect Dis. 2021 Jan;27(1):104-112
pubmed: 33350904
Arch Virol. 2019 Jul;164(7):1949-1965
pubmed: 31065850
Antiviral Res. 2017 Oct;146:112-120
pubmed: 28842265
Am J Trop Med Hyg. 2018 Jan;98(1):211-215
pubmed: 29165231
Global Health. 2017 Feb 8;13(1):9
pubmed: 28179007
Viruses. 2020 Jun 25;12(6):
pubmed: 32630501
J Virol. 2021 Nov 23;95(24):e0163821
pubmed: 34613808
PLoS Negl Trop Dis. 2020 Nov 24;14(11):e0008863
pubmed: 33232320
Int J Dermatol. 2017 Mar;56(3):252-259
pubmed: 27696381
J Virol. 2018 Oct 12;92(21):
pubmed: 30111561
PLoS Pathog. 2019 Sep 26;15(9):e1008050
pubmed: 31557262
Sci Adv. 2019 Jul 10;5(7):eaaw9535
pubmed: 31309159
J Gen Virol. 2010 Jan;91(Pt 1):199-207
pubmed: 19812264
PLoS Pathog. 2022 May 19;18(5):e1010485
pubmed: 35587473
Antiviral Res. 2004 Apr;62(1):21-5
pubmed: 15026198
Virol Sin. 2022 Feb;37(1):127-137
pubmed: 35234630
PLoS Pathog. 2019 Dec 2;15(12):e1008183
pubmed: 31790513
Antiviral Res. 2013 Oct;100(1):159-89
pubmed: 23906741
Vet Res. 2010 May-Jun;41(3):33
pubmed: 20167195
Cancer Res. 1982 Sep;42(9):3858-63
pubmed: 6286115
Antiviral Res. 2015 Jun;118:75-81
pubmed: 25796972
PLoS Negl Trop Dis. 2018 Jul 16;12(7):e0006628
pubmed: 30011277
PLoS Negl Trop Dis. 2020 Apr 6;14(4):e0008105
pubmed: 32251473
Antiviral Res. 2018 Feb;150:137-147
pubmed: 29199036
Front Cell Infect Microbiol. 2021 Mar 24;11:648077
pubmed: 33869079
Cell Rep. 2017 Sep 5;20(10):2396-2407
pubmed: 28877473
Emerg Infect Dis. 2018 Sep;24(9):
pubmed: 30124424
EBioMedicine. 2022 Aug;82:104188
pubmed: 35907368
PLoS Negl Trop Dis. 2020 Aug 13;14(8):e0008637
pubmed: 32790668
J Virol. 2020 Mar 31;94(8):
pubmed: 31996434
J Infect Dis. 2013 Jun 15;207(12):1909-21
pubmed: 23417661
Virology. 1959 Jul;8(3):396-7
pubmed: 13669362
J Biol Chem. 2011 Feb 4;286(5):3227-34
pubmed: 21123175
PLoS Pathog. 2015 May 01;11(5):e1004879
pubmed: 25933376
J Virol. 2017 Apr 28;91(10):
pubmed: 28250124
J Virol Methods. 2013 Jan;187(1):26-31
pubmed: 22906394
Curr Opin Virol. 2019 Feb;34:70-78
pubmed: 30660091
J Virol. 2010 Nov;84(21):11089-100
pubmed: 20739514
PLoS One. 2012;7(1):e29712
pubmed: 22238639