Spectral focusing-based stimulated Raman scattering microscopy using compact glass blocks for adjustable dispersion.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Jun 2023
01 Jun 2023
Historique:
received:
14
02
2023
revised:
09
04
2023
accepted:
11
04
2023
medline:
21
6
2023
pubmed:
21
6
2023
entrez:
21
6
2023
Statut:
epublish
Résumé
Spectral focusing is a well-established technique for increasing spectral resolution in coherent Raman scattering microscopy. However, current methods for tuning optical chirp in setups using spectral focusing, such as glass rods, gratings, and prisms, are very cumbersome, time-consuming to use, and difficult to align, all of which limit more widespread use of the spectral focusing technique. Here, we report a stimulated Raman scattering (SRS) configuration which can rapidly tune optical chirp by utilizing compact adjustable-dispersion TIH53 glass blocks. By varying the height of the blocks, the number of bounces in the blocks and therefore path length of the pulses through the glass can be quickly modulated, allowing for a convenient method of adjusting chirp with almost no necessary realignment. To demonstrate the flexibility of this configuration, we characterize our system's signal-to-noise ratio and spectral resolution at different chirp values and perform imaging in both the carbon-hydrogen stretching region (MCF-7 cells) and fingerprint region (prostate cores). Our findings show that adjustable-dispersion glass blocks allow the user to effortlessly modify their optical system to suit their imaging requirements. These blocks can be used to significantly simplify and miniaturize experimental configurations utilizing spectral focusing.
Identifiants
pubmed: 37342685
doi: 10.1364/BOE.486753
pii: 486753
pmc: PMC10278629
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2510-2522Informations de copyright
© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
J Biomed Opt. 2019 Apr;24(4):1-8
pubmed: 31007003
Science. 2010 Dec 3;330(6009):1368-70
pubmed: 21127249
Biomed Eng Online. 2003 May 17;2:13
pubmed: 12801419
Nat Methods. 2019 Sep;16(9):830-842
pubmed: 31471618
Nat Commun. 2021 May 24;12(1):3052
pubmed: 34031374
Cell Stem Cell. 2017 Mar 2;20(3):303-314.e5
pubmed: 28041894
Opt Express. 2020 May 11;28(10):15663-15677
pubmed: 32403589
J Vis Exp. 2022 Feb 1;(180):
pubmed: 35188120
Biomed Opt Express. 2018 Nov 08;9(12):6116-6131
pubmed: 31065417
Opt Express. 2020 Sep 28;28(20):30210-30221
pubmed: 33114904
Opt Express. 2015 Sep 21;23(19):25235-46
pubmed: 26406721
PLoS One. 2016 May 25;11(5):e0156371
pubmed: 27224203
Opt Express. 2009 Feb 16;17(4):2984-96
pubmed: 19219203
Anal Chem. 2019 Aug 6;91(15):9333-9342
pubmed: 31287649
Cell Death Dis. 2020 Nov 23;11(11):1003
pubmed: 33230108
Nat Biomed Eng. 2017;1:
pubmed: 28955599
Cancers (Basel). 2022 Feb 06;14(3):
pubmed: 35159086
J Phys Chem B. 2013 Apr 25;117(16):4634-40
pubmed: 23256635
J Phys Chem B. 2006 Mar 30;110(12):5854-64
pubmed: 16553391
Nat Commun. 2020 Sep 24;11(1):4830
pubmed: 32973134
J Am Chem Soc. 2017 Jan 18;139(2):583-586
pubmed: 28027644
Opt Lett. 2020 Apr 15;45(8):2299-2302
pubmed: 32287218
Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11624-9
pubmed: 26324899
Sci Rep. 2019 Jul 11;9(1):10052
pubmed: 31296917
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
PLoS Med. 2020 Aug 14;17(8):e1003281
pubmed: 32797086
Opt Express. 2018 Apr 16;26(8):10230-10241
pubmed: 29715963
Biomed Opt Express. 2022 Jan 27;13(2):995-1004
pubmed: 35284158
Front Mol Biosci. 2014 Nov 17;1:24
pubmed: 25988165