Vacuum-assisted tissue embedding for whole-heart imaging.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Jun 2023
01 Jun 2023
Historique:
received:
28
02
2023
revised:
25
04
2023
accepted:
26
04
2023
medline:
21
6
2023
pubmed:
21
6
2023
entrez:
21
6
2023
Statut:
epublish
Résumé
The use of combined optical imaging and tissue sectioning has potential for use in visualizing heart-wide fine structures at single-cell resolution. However, existing tissue preparation methods fail to generate ultrathin cavity-containing cardiac tissue slices with minimal deformation. This study developed an efficient vacuum-assisted tissue embedding method to prepare high-filled, agarose-embedded whole-heart tissue. Utilizing optimized vacuum parameters, we achieved 94% filled whole-heart tissue with the thinnest cut slice of 5 µm. We subsequently imaged a whole mouse heart sample using vibratome-integrated fluorescence micro-optical sectioning tomography (fMOST) with a voxel size of 0.32 µm × 0.32 µm × 1 µm. The imaging results indicated that the vacuum-assisted embedding method enabled whole-heart tissue to withstand long-term thin cutting while ensuring that slices were consistent and of high quality.
Identifiants
pubmed: 37342702
doi: 10.1364/BOE.488766
pii: 488766
pmc: PMC10278630
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2539-2550Informations de copyright
© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
J. Chen, Y. Li, R. Xie, J. Yuan, and Q. Luo have filed patent applications based on this research.
Références
Circulation. 2010 Nov 9;122(19):1973-4
pubmed: 21060087
J Am Coll Cardiol. 2006 Nov 21;48(10):1988-2001
pubmed: 17112989
iScience. 2022 Jul 20;25(8):104805
pubmed: 35992061
Biomed Opt Express. 2016 Aug 29;7(9):3716-3720
pubmed: 27699132
Nat Protoc. 2021 Jun;16(6):2732-2748
pubmed: 34021294
J Opt Soc Am A. 1990 Oct;7(10):2032-40
pubmed: 2231113
Front Physiol. 2019 Jun 25;10:755
pubmed: 31293436
iScience. 2021 Apr 01;24(4):102387
pubmed: 33981974
Front Cardiovasc Med. 2022 Aug 04;9:945198
pubmed: 35990991
Science. 2008 Dec 5;322(5907):1494-7
pubmed: 19056974
IEEE J Transl Eng Health Med. 2016 Jun 15;4:1800210
pubmed: 27574574
JACC Cardiovasc Imaging. 2011 Sep;4(9):1022-39
pubmed: 21920342
J Biomed Opt. 2013 Nov;18(11):117010
pubmed: 24247811
iScience. 2020 Jun 26;23(6):101140
pubmed: 32460006
Prog Biophys Mol Biol. 2022 Jan;168:10-17
pubmed: 34358555
iScience. 2020 Aug 21;23(8):101432
pubmed: 32805648
Biomed Opt Express. 2019 Apr 29;10(5):2612-2622
pubmed: 31149384
iScience. 2021 Aug 25;24(9):103016
pubmed: 34522859
Nat Methods. 2021 Mar;18(3):309-315
pubmed: 33649587
Circ Res. 2022 Feb 4;130(3):352-365
pubmed: 34995101