Structural disruption of Ntox15 nuclease effector domains by immunity proteins protects against type VI secretion system intoxication in Bacteroidales.
Bacteroides
inflammatory bowel disease
microbiome
structural biology
type VI secretion system
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
31 Aug 2023
31 Aug 2023
Historique:
pubmed:
22
6
2023
medline:
22
6
2023
entrez:
22
6
2023
Statut:
ppublish
Résumé
Bacteroidales use type VI secretion systems (T6SS) to competitively colonize and persist in the colon. We identify a horizontally transferred T6SS with Ntox15 family nuclease effector (Tde1) that mediates interbacterial antagonism among Bacteroidales, including several derived from a single human donor. Expression of cognate (Tdi1) or orphan immunity proteins in acquired interbacterial defense systems protects against Tde1-dependent attack. We find that immunity protein interaction induces a large effector conformational change in Tde nucleases, disrupting the active site and altering the DNA-binding site. Crystallographic snapshots of isolated Tde1, the Tde1/Tdi1 complex, and homologs from
Identifiants
pubmed: 37345922
doi: 10.1128/mbio.01039-23
pmc: PMC10470768
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0103923Subventions
Organisme : NIAID NIH HHS
ID : K08 AI159619
Pays : United States
Organisme : NIAID NIH HHS
ID : K08 AI159619
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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