General Gel-sol Method to Synthesize Various Highly Fluorescent Nanoclusters and Assay of Nuclease with the Near Infrared-emitting Gold Nanoclusters.

Fluorescence Metal nanoclusters Nuclease activity Nuclease inhibition S1 nuclease

Journal

Journal of fluorescence

ISSN: 1573-4994

Titre abrégé: J Fluoresc

Pays: Netherlands

ID NLM: 9201341

Informations de publication

Date de publication:
22 Jun 2023

Historique:

received: 06 05 2023

accepted: 05 06 2023

medline: 22 6 2023

pubmed: 22 6 2023

entrez: 22 6 2023

Statut: aheadofprint

Résumé

A general egg white gel-sol strategy for fabrication of highly fluorescent Au, Ag, Cu, and Pt nanoclusters (NCs) and the first example of using Au NCs for assay of nuclease activity and inhibition were described. The Au NCs enabled bright red fluorescence, and the other Ag, Cu, and Pt NCs have highly blue emission. The red-emitting Au NCs were further applied in assay of S1 nuclease activity and inhibition. Free hemin efficiently quenches the emission of Au NCs by photoinduced electron transfer due to the formation of Au NCs-hemin conjugates. However, G-quadruplex/hemin exerts negligible effect on its fluorescence due to no Au NCs-hemin conjugate formed. There are stronger electrostatic repulsion effects between both negatively charged G-quadruplex and Au NCs. Therefore, a novel G-quadruplex/hemin-based Au NCs fluorescent sensor for S1 nuclease was designed. A known G-rich oligonucleotide (ODN) serves as not only substrate for S1 nuclease but also for the construction of G-quadruplex/hemin. The G-rich ODN is hydrolyzed into fragments by S1 nuclease resulting in no G-quadruplex/hemin formation. Therefore, the free hemin quenches Au NCs fluorescence remarkably and the assay of S1 nuclease activity and inhibition has accomplished. Both the fluorescent NCs syntheses and the detection of S1 nuclease are facile and efficient.

Identifiants

DOI: 10.1007/s10895-023-03296-y PMID: 37347423

pubmed: 37347423

doi: 10.1007/s10895-023-03296-y

pii: 10.1007/s10895-023-03296-y

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Informations de copyright

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