Superresolution Fluorescence Microscopy of Platelet Subcellular Structures as a Potential Tumor Liquid Biopsy.
platelet subcellular structures
superresolution fluorescence microscopy
tumor liquid biopsy
Journal
Small methods
ISSN: 2366-9608
Titre abrégé: Small Methods
Pays: Germany
ID NLM: 101724536
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
25
04
2023
medline:
23
10
2023
pubmed:
23
6
2023
entrez:
23
6
2023
Statut:
ppublish
Résumé
Blood-based tumor liquid biopsies are promising as an alternative or complement to tissue biopsies due to their noninvasiveness, convenience, and safety, and there is still a great demand for the discovery of new biomarkers for these biopsies. Here, nanoscale distribution patterns of subcellular structures in platelets, as imaged by structured illumination superresolution fluorescence microscopy, as a new type of potential biomarker for tumor liquid biopsies are presented. A standardized protocol for platelet sample preparation and developed an automated high-throughput image analysis workflow is established. The diagnostic capability based on the statistical analysis of 280 000 superresolution images of individual platelets from a variety of tumor patients, benign mass patients, and healthy volunteers (n = 206) is explored. These results suggest that the nanoscale distribution patterns of α-granules in platelets have the potential to be biomarkers for several cancers, including glioma and cervical, endometrial, and ovarian cancers, facilitating not only diagnosis but also therapeutic monitoring. This study provides a promising novel type of platelet parameter for tumor liquid biopsies at the subcellular level rather than the existing cellular or molecular level and opens up a new avenue for clinical applications of superresolution imaging techniques.
Identifiants
pubmed: 37349902
doi: 10.1002/smtd.202300445
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300445Subventions
Organisme : National Key R&D Program of China
ID : 2022YFC3401100
Organisme : National Natural Science Foundation of China
ID : 92054110
Organisme : National Natural Science Foundation of China
ID : 32271428
Organisme : National Natural Science Foundation of China
ID : 81873430
Organisme : National Natural Science Foundation of China
ID : 81974414
Organisme : National Natural Science Foundation of China
ID : 31600692
Organisme : National Natural Science Foundation of China
ID : 61827825
Organisme : Key R&D Program of Hubei province
ID : 2022BCA027
Organisme : Hubei Province Health and Family Planning Scientific Research Project
ID : WJ2023M138
Organisme : Science Fund for Creative Research Group of China
ID : 61721092
Organisme : Director Fund of WNLO
Informations de copyright
© 2023 The Authors. Small Methods published by Wiley-VCH GmbH.
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