Single-cell analysis reveals the multiple patterns of immune escape in the nasopharyngeal carcinoma microenvironment.
Journal
Clinical and translational medicine
ISSN: 2001-1326
Titre abrégé: Clin Transl Med
Pays: United States
ID NLM: 101597971
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
revised:
09
06
2023
received:
19
01
2023
accepted:
15
06
2023
medline:
26
6
2023
pubmed:
23
6
2023
entrez:
23
6
2023
Statut:
ppublish
Résumé
Single-cell transcriptomics has revolutionised our understanding of the cellular composition of the tumour microenvironment (TME) in nasopharyngeal carcinoma (NPC). Despite this progress, a key limitation of this technique has been its inability to capture epithelial/tumour cells, which has hindered further investigation of tumour heterogeneity and immune escape in NPC. In this study, we aimed to address these limitations by analysing the transcriptomics and spatial characteristics of NPC tumour cells at single-cell resolution using scRNA/snRNA-seq and imaging mass cytometry techniques. Our findings demonstrate multiple patterns of immune escape mechanisms in NPC, including the loss of major histocompatibility complex (MHC) molecules in malignant cells, induction of epithelial-mesenchymal transition in fibroblast-like malignant cells and the use of hyperplastic cells in tumour nests to protect tumour cells from immune infiltration. Additionally, we identified, for the first time, a CD8+ natural killer (NK) cell cluster that is specific to the NPC TME. These findings provide new insights into the complexity of NPC immune landscape and may lead to novel therapeutic strategies for this disease.
Sections du résumé
BACKGROUND
BACKGROUND
Single-cell transcriptomics has revolutionised our understanding of the cellular composition of the tumour microenvironment (TME) in nasopharyngeal carcinoma (NPC). Despite this progress, a key limitation of this technique has been its inability to capture epithelial/tumour cells, which has hindered further investigation of tumour heterogeneity and immune escape in NPC.
METHODS
METHODS
In this study, we aimed to address these limitations by analysing the transcriptomics and spatial characteristics of NPC tumour cells at single-cell resolution using scRNA/snRNA-seq and imaging mass cytometry techniques.
RESULTS
RESULTS
Our findings demonstrate multiple patterns of immune escape mechanisms in NPC, including the loss of major histocompatibility complex (MHC) molecules in malignant cells, induction of epithelial-mesenchymal transition in fibroblast-like malignant cells and the use of hyperplastic cells in tumour nests to protect tumour cells from immune infiltration. Additionally, we identified, for the first time, a CD8+ natural killer (NK) cell cluster that is specific to the NPC TME.
CONCLUSIONS
CONCLUSIONS
These findings provide new insights into the complexity of NPC immune landscape and may lead to novel therapeutic strategies for this disease.
Identifiants
pubmed: 37349991
doi: 10.1002/ctm2.1315
pmc: PMC10288070
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1315Subventions
Organisme : Universities Stable Funding Key Projects
ID : WDZC20200821104802001
Organisme : State Key Laboratory of Chemical Oncogenomics
Organisme : Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory
Organisme : Shenzhen Science and Technology research and development funds
ID : JCYJ20200109143018683
Organisme : Natural Science Foundation of Guangdong Province of China
ID : 2021A1515011029
Informations de copyright
© 2023 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.
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