Protocol for extracting live blastoderm cells from embryos of annual killifish.

Cell isolation Developmental biology Model Organisms

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
22 Jun 2023
Historique:
received: 24 02 2023
revised: 04 04 2023
accepted: 10 05 2023
medline: 23 6 2023
pubmed: 23 6 2023
entrez: 23 6 2023
Statut: aheadofprint

Résumé

The implementation of in vitro approaches using undifferentiated embryonic cells from annual killifish to complement existing in vivo developmental studies has been hindered by a lack of efficient isolation techniques. Here, we present a protocol to isolate annual killifish blastoderm cells, at the epiboly and early dispersion phase, from embryos. We describe steps for hair removal, embryo cleaning, dechorionation, and cell purification. This protocol may also be used to develop strategies to isolate cells from embryos presenting similar challenges.

Identifiants

pubmed: 37352104
pii: S2666-1667(23)00311-8
doi: 10.1016/j.xpro.2023.102344
pmc: PMC10320274
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102344

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing financial interests.

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Auteurs

Sebastian Vásquez-Sepúlveda (S)

Laboratory for Mechanobiology of Transforming Systems, Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 7820436, Chile; Laboratory for Molecular Mechanics of Cell Adhesion, Department of Physiology Pontificia Universidad Católica de Chile, Santiago 8320165, Chile.

Nestor Guerrero (N)

Integrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.

Carmen Gloria Lemus (CG)

Integrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.

Margarita Meynard (M)

Integrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile; Center for Geroscience, Brain Health and Metabolism, Santiago 8380453, Chile.

Mauricio Cerda (M)

Integrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.

Miguel Luis Concha (ML)

Integrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile; Center for Geroscience, Brain Health and Metabolism, Santiago 8380453, Chile.

Cristina Bertocchi (C)

Laboratory for Molecular Mechanics of Cell Adhesion, Department of Physiology Pontificia Universidad Católica de Chile, Santiago 8320165, Chile; Graduate School of Engineering Science, Osaka University, Osaka 565-0871, Japan. Electronic address: cbertocchi@bio.puc.cl.

Andrea Ravasio (A)

Laboratory for Mechanobiology of Transforming Systems, Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 7820436, Chile. Electronic address: ravasio@gmail.com.

Classifications MeSH