Tylophora indica (Burm. f.) Merr alleviates tracheal smooth muscle hyperresponsiveness in ovalbumin-induced allergic-asthma model in guinea-pigs: Evidences from ex vivo, in silico and in vivo studies.
H1 receptor
M3 receptor
asthma
molecular docking
tracheal smooth muscle relaxant
Journal
Fundamental & clinical pharmacology
ISSN: 1472-8206
Titre abrégé: Fundam Clin Pharmacol
Pays: England
ID NLM: 8710411
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
23
05
2023
received:
30
12
2022
accepted:
05
06
2023
medline:
27
11
2023
pubmed:
24
6
2023
entrez:
24
6
2023
Statut:
ppublish
Résumé
Tylophora indica (Burm. f.) Merr is a climbing perennial plant reported in Indian traditional system of medicine for its use in allergy and asthma. However, only few scientific studies have been performed in the past to validate its antiasthmatic potential. The present study deals with investigation of airway smooth muscle relaxant and antiasthmatic potential of extract and subsequent fractions prepared from T. indica. The most active fraction of T. indica leaves selected through bio-guided activity was subjected to liquid chromatography-mass spectrometry (LC-MS) analysis for chemical profiling. The binding affinity of identified compounds in fraction towards M F-2 was found most effective in bioassay-guided fractionation. Characterization by LC-MS analysis revealed presence of five major bioactive compounds in F-2 that showed good docking interactions with M These results confirm the traditional use of T. indica as an antiasthmatic agent which are evidenced through ex vivo, in silico and in vivo studies.
Sections du résumé
BACKGROUND
BACKGROUND
Tylophora indica (Burm. f.) Merr is a climbing perennial plant reported in Indian traditional system of medicine for its use in allergy and asthma. However, only few scientific studies have been performed in the past to validate its antiasthmatic potential.
OBJECTIVES
OBJECTIVE
The present study deals with investigation of airway smooth muscle relaxant and antiasthmatic potential of extract and subsequent fractions prepared from T. indica.
METHODS
METHODS
The most active fraction of T. indica leaves selected through bio-guided activity was subjected to liquid chromatography-mass spectrometry (LC-MS) analysis for chemical profiling. The binding affinity of identified compounds in fraction towards M
RESULTS
RESULTS
F-2 was found most effective in bioassay-guided fractionation. Characterization by LC-MS analysis revealed presence of five major bioactive compounds in F-2 that showed good docking interactions with M
CONCLUSION
CONCLUSIONS
These results confirm the traditional use of T. indica as an antiasthmatic agent which are evidenced through ex vivo, in silico and in vivo studies.
Substances chimiques
Ovalbumin
9006-59-1
Anti-Asthmatic Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1153-1169Informations de copyright
© 2023 Société Française de Pharmacologie et de Thérapeutique. Published by John Wiley & Sons Ltd.
Références
Network GA. The Global Asthma Report 2014; 2014. http://globalasthmareport.org/burden/burden.php
He R, Wang S, Yang S, et al. Shaoyao-Gancao-Tang regulates the T-helper-type 1/T-helper-type 2 ratio in the lung and gut and alters gut microbiota in rats with ovalbumin-induced asthma. J Ethnopharmacol. 2023;309(March):116300. doi:10.1016/j.jep.2023.116300
Chen Y, Wu H, Li Y, et al. Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation. Biomed Pharmacother. 2020;130(July):110616. doi:10.1016/j.biopha.2020.110616
Li J, Li J, Zhang F. The immunoregulatory effects of traditional Chinese medicine on treatment of asthma or asthmatic inflammation. Am J Chin Med. 2015;43(6):1059-1081. doi:10.1142/S0192415X15500615
Lee SY, Bae CS, Seo NS, et al. Camellia japonica oil suppressed asthma occurrence via GATA-3 & IL-4 pathway and its effective and major component is oleic acid. Phytomedicine. 2019;57:84-94. doi:10.1016/J.PHYMED.2018.12.004
Kim HH, Yoo JS, Shin TY, Kim SH. Aqueous extract of Mosla chinensis inhibits mast cell-mediated allergic inflammation. Am J Chin Med. 2012;40(6):1257-1270. doi:10.1142/S0192415X12500930
Vieira GC, De Lima JF, De Figueiredo RCBQ, Mascarenhas SR, Bezerra-Santos CR, Piuvezam MR. Inhaled Cissampelos sympodialis down-regulates airway allergic reaction by reducing lung CD3+ T cells. Phytother Res. 2013;27(6):916-925. doi:10.1002/PTR.4791
Bisht A, Jain S, Misra A, Dwivedi J, Paliwal S, Sharma S. Cedrus deodara (Roxb. ex D.Don) G.Don: a review of traditional use, phytochemical composition and pharmacology. J Ethnopharmacol. 2021;279:114361. doi:10.1016/j.jep.2021.114361
Gururani R, Patel S, Yaduvanshi N, Dwivedi J, Paliwal S, Sharma S. Tylophora indica (Burm. f.) merr: an insight into phytochemistry and pharmacology. J Ethnopharmacol. 2020;262:113122. doi:10.1016/J.JEP.2020.113122
Udupa AL, Udupa SL, Guruswamy MN. The possible site of anti-asthmatic action of Tylophora asthmatica on pituitary-adrenal axis in albino rats. Planta Med. 1991;57(5):409-413. doi:10.1055/s-2006-960136
Pratama NP, Wulandari S, Nugroho AE, Fakhrudin N, Astuti P, Sudarsono. Tylophorine abrogates G2/M arrest induced by doxorubicine and promotes increased apoptosis in T47D breast cancer cells. Asian Pacific J Cancer Prev. 2018;19(11):3065-3069. doi:10.31557/APJCP.2018.19.11.3065
Monji F, Adaikan PG, Lau LC, et al. Role of the serotonergic pathway in uterotonic activity of Ananas comosus (L.) Merr.-an in vitro and in vivo study. Phytomedicine. 2018;48:32-42. doi:10.1016/J.PHYMED.2018.05.004
Arias-Durán L, Estrada-Soto S, Hernández-Morales M, et al. Tracheal relaxation through calcium channel blockade of Achillea millefolium hexanic extract and its main bioactive compounds. J Ethnopharmacol. 2020;253(February):112643. doi:10.1016/j.jep.2020.112643
Sharma D, Patel S, Verma K, et al. Antibacterial and antidiarrheal activity of Butea monospermea bark extract against waterborne enterobacter Cloacae in rodents: in-vitro, ex-vivo and in-vivo evidences. J Ethnopharmacol. 2019;241:112014. doi:10.1016/J.JEP.2019.112014
Khan I, Rahman H, Abd El-Salam NM, et al. Punica granatum peel extracts: HPLC fractionation and LC MS analysis to quest compounds having activity against multidrug resistant bacteria. BMC Complement Altern Med. 2017;17(1):1-6. doi:10.1186/s12906-017-1766-4
Chakrabarty N, Haque MT, Ershad M, et al. Antidiarrheal activity of methanol extract of Piper sylvaticum (roxb.) stem in mice and in silico molecular docking of its isolated compounds. Discov Phytomedicine. 2019;6(3):92-98. doi:10.15562/phytomedicine.2019.97
Patel S, Gururani R, Jain S, et al. Repurposing of digoxin in pain and inflammation: an evidence-based study. Drug Dev Res. 2022;83(5):1097-1110. doi:10.1002/ddr.21935
Vilela DAD, Silva BAO, Brito MC, et al. Lippia alnifolia essential oil induces relaxation on guinea-pig trachea by multiple pathways. J Ethnopharmacol. 2020;246:112162. doi:10.1016/j.jep.2019.112162
Guidelines O, The FOR, Of T. OECD guidelines for the testing of chemicals. 2008;(October).
Molitorisova M, Sutovska M, Kazimierova I, et al. The anti-asthmatic potential of flavonol kaempferol in an experimental model of allergic airway inflammation. Eur J Pharmacol. 2021;891:173698. doi:10.1016/j.ejphar.2020.173698
Jang YW, Lee JY, Kim CJ. Anti-asthmatic activity of phenolic compounds from the roots of Gastrodia elata Bl. Int Immunopharmacol. 2010;10(2):147-154. doi:10.1016/j.intimp.2009.10.009
Rani NZA, Lam KW, Jalil J, Mohamad HF, Ali MSM, Husain K. Mechanistic studies of the antiallergic activity of Phyllanthus amarus schum. & thonn. and its compounds. Molecules. 2021;26(3):695. doi:10.3390/molecules26030695
Balkrishna A, Solleti SK, Singh H, Tomer M, Sharma N, Varshney A. Calcio-herbal formulation, Divya-Swasari-Ras, alleviates chronic inflammation and suppresses airway remodelling in mouse model of allergic asthma by modulating pro-inflammatory cytokine response. Biomed Pharmacother. 2020;126(February):110063. doi:10.1016/j.biopha.2020.110063
An M, Oh M, Park KT, et al. Anti-asthma and antitussive effects of a fermented extract of a mixture of Ramulus mori, Anthriscus sylvestris, and Salvia plebeian. Food Sci Biotechnol. 2021;30(9):1257-1268. doi:10.1007/s10068-021-00955-3
de Morel LJF, de Azevedo BC, Carmona F, et al. A standardized methanol extract of Eclipta prostrata (L.) L. (Asteraceae) reduces bronchial hyperresponsiveness and production of Th2 cytokines in a murine model of asthma. J Ethnopharmacol. 2017;198:226-234. doi:10.1016/J.JEP.2016.12.008
Sánchez-Recillas A, Rivero-Medina L, Ortiz-Andrade R, Araujo-León JA, Flores-Guido JS. Airway smooth muscle relaxant activity of Cordia dodecandra A. DC. mainly by cAMP increase and calcium channel blockade. J Ethnopharmacol. 2019;229:280-287. doi:10.1016/J.JEP.2018.10.013
Ribeiro-Filho J, Piuvezam MR, Bozza PT. Anti-allergic properties of curine, a bisbenzylisoquinoline alkaloid. Molecules. 2015;20(3):4695-4707. doi:10.3390/molecules20034695
Fu M, Zou B, An K, et al. Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata ‘Chachi’). Food Funct. 2019;10(2):903-911. doi:10.1039/c8fo01753k
Ganguly T, Sainis KB. Inhibition of cellular immune responses by Tylophora indica in experimental models. Phytomedicine. 2001;8(5):348-355. doi:10.1078/0944-7113-00055
Shivpuri DN, Menon MPS, Prakash D. A crossover double-blind study on Tylophora indica in the treatment of asthma and allergic rhinitis. J Allergy. 1969;43(3):145-150. doi:10.1016/S0021-8707(69)80005-5
Ali M, Bhutani KK. Alkaloids from Tylophora indica. Phytochemistry. 1989;28(12):3513-3517. doi:10.1016/0031-9422(89)80376-0
Kaur H, Singh K. A brief phytopharmacological overview of Tylophora Indica-an endangered medicinal plant. International Journal of Pharmaceutical Sciences and Research. 2012;3(11):4073-4076. www.ijpsr.com
Rao KN, Venkatachalam SR. Inhibition of dihydrofolate reductase and cell growth activity by the phenanthroindolizidine alkaloids pergularinine and tylophorinidine: the in vitro cytotoxicity of these plant alkaloids and their potential as antimicrobial and anticancer agents. Toxicol Vitr. 2000;14(1):53-59. doi:10.1016/S0887-2333(99)00092-2
Saraswati S, Kanaujia PK, Kumar S, Kumar R, Alhaider AA. Tylophorine, a phenanthraindolizidine alkaloid isolated from Tylophora indica exerts antiangiogenic and antitumor activity by targeting vascular endothelial growth factor receptor 2-mediated angiogenesis. Mol Cancer. 2013;12(1):1-16. doi:10.1186/1476-4598-12-82
Kareti SR, Pharm SM. In silico molecular docking analysis of potential anti-Alzheimer's compounds present in chloroform extract of Carissa carandas leaf using gas chromatography MS/MS. Curr Ther Res - Clin Exp. 2020;93:100615. doi:10.1016/j.curtheres.2020.100615
Menezes PMN, Brito MC, de Paiva GO, et al. Relaxant effect of Lippia origanoides essential oil in guinea-pig trachea smooth muscle involves potassium channels and soluble guanylyl cyclase. J Ethnopharmacol. 2018;220:16-25. doi:10.1016/j.jep.2018.03.040
Lebender LF, Prünte L, Rumzhum NN, Ammit AJ. Selectively targeting prostanoid E (EP) receptor-mediated cell signalling pathways: implications for lung health and disease. Pulm Pharmacol Ther. 2018;49:75-87. doi:10.1016/J.PUPT.2018.01.008
Ma Y, Yang XH, Tu Y, Athari SS. Survey the effect of drug treatment on modulation of cytokines gene expression in allergic rhinitis. Fundam Clin Pharmacol. 2023;37(2):340-346. doi:10.1111/FCP.12847
Dhakal H, Yang E, Lee S, et al. Avenanthramide C from germinated oats exhibits anti-allergic inflammatory effects in mast cells. Sci Rep. 9(1):6884. doi:10.1038/s41598-019-43412-2