Protocol for high-throughput electron tomography exemplified on centrioles in primary human plasma cells.

High Throughput Screening Microscopy

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
22 Jun 2023
Historique:
received: 14 03 2023
revised: 26 04 2023
accepted: 21 05 2023
medline: 24 6 2023
pubmed: 24 6 2023
entrez: 24 6 2023
Statut: aheadofprint

Résumé

Electron microscopy is the gold standard to characterize cellular ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. Here, we describe a semi-automated high-throughput strategy using single-axis serial section electron tomography to investigate and analyze centriole ultrastructure in bone-marrow-derived, primary human CD138

Identifiants

DOI: 10.1016/j.xpro.2023.102373 PMID: 37354457 PMC: PMC10320260
pubmed: 37354457
pii: S2666-1667(23)00340-4
doi: 10.1016/j.xpro.2023.102373
pmc: PMC10320260
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102373

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests A.K. received honoraria from Hoffmann-La Roche, Bayer, Daiichi Sankyo, and AbbVie and research funding from Bayer and Merck and is a paid consultant for Hoffmann-La Roche, Daiichi Sankyo, Bristol Myers Squibb, and AbbVie.

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Auteurs

Sebastian Köhrer (S)

Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), and Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany. Electronic address: sebastian.koehrer@embl.de.

Tobias Dittrich (T)

Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), and Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany; Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany; Amyloidosis Center, University of Heidelberg, 69120 Heidelberg, Germany.

Martin Schorb (M)

Electron Microscopy Core Facility, European Molecular Biology Laboratory, 69117 Heidelberg, Germany. Electronic address: martin.schorb@embl.de.

Isabella Haberbosch (I)

Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), and Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany; Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany.

Yannick Schwab (Y)

Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany; Electron Microscopy Core Facility, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.

Alwin Krämer (A)

Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ), and Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany; Department of Internal Medicine V, University of Heidelberg, 69120 Heidelberg, Germany. Electronic address: a.kraemer@dkfz-heidelberg.de.

Classifications MeSH