Label-free electrochemical immunosensor based on staphylococcal protein a and AgNPs-rGO-Nf for sensitive detection of virginiamycin M1.
Animals
Humans
Swine
Electrochemical Techniques
/ methods
Staphylococcal Protein A
Streptogramin A
Biosensing Techniques
/ methods
Reproducibility of Results
Metal Nanoparticles
/ chemistry
Immunoassay
/ methods
Silver
Graphite
/ chemistry
Nanocomposites
/ chemistry
Gold
/ chemistry
Antibodies
Limit of Detection
AgNPs-rGO
Electrochemical immunosensor
Label-free
SPA
VIR M1
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
11
04
2023
revised:
28
05
2023
accepted:
09
06
2023
medline:
24
7
2023
pubmed:
25
6
2023
entrez:
24
6
2023
Statut:
ppublish
Résumé
Virginiamycin (VIR), a feed additive, is used to promote pig and poultry growth. However, it is hazardous to human health. This work described a label-free electrochemical immunosensor based on silver nanoparticles-reduced graphene oxide (AgNPs-rGO) nanocomposites and staphylococcal protein A (SPA) for the first time to directly detect the residual marker VIR M1. Good catalytic currents for oxygen reduction reaction were apparently obtained after the modification of nanocomposites on gold electrode. Nanocomposites were characterized using UV-Vis, X-ray diffraction (XRD) patterns, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). SPA was targeted to immobilize VIR M1 monoclonal antibody (mAb) by binding to Fc region of antibody. The proposed immunosensor showed a wide linear range from 0.25 ng mL
Identifiants
pubmed: 37354640
pii: S1567-5394(23)00126-3
doi: 10.1016/j.bioelechem.2023.108489
pii:
doi:
Substances chimiques
graphene oxide
0
Staphylococcal Protein A
0
Streptogramin A
8W4UOL59AZ
Silver
3M4G523W1G
Graphite
7782-42-5
Gold
7440-57-5
Antibodies
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108489Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.