Identification of ORM1, vWF, SPARC, and PPBP as immune-related proteins involved in immune thrombocytopenia by quantitative LC-MS/MS.
Immune thrombocytopenia
LC-MS/MS
TNF-α
Journal
Clinical proteomics
ISSN: 1542-6416
Titre abrégé: Clin Proteomics
Pays: England
ID NLM: 101184586
Informations de publication
Date de publication:
24 Jun 2023
24 Jun 2023
Historique:
received:
09
11
2021
accepted:
03
06
2023
medline:
25
6
2023
pubmed:
25
6
2023
entrez:
24
6
2023
Statut:
epublish
Résumé
Immune thrombocytopenia (ITP) is a common autoimmune disease characterized by loss of immune tolerance to platelet autoantigens leading to excessive destruction and insufficient production of platelets. Quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed to detect the differentially expressed proteins in bone marrow samples from active ITP patients and normal controls. Our bioinformatic analysis identified two upregulated proteins (ORM1 and vWF) and two downregulated proteins (PPBP and SPARC) related to immune function. The four proteins were all found to be related to the tumor necrosis factor (TNF) -α signalling pathway and involved in the pathogenesis of ITP in KEGG pathway analysis. Bioinformatics analysis identified differentially expressed proteins in bone marrow that are involved in the TNF-α signalling pathway and are related to the activation of immune function in ITP patients. These findings could provide new ideas for research on the loss of immune tolerance in ITP patients.
Sections du résumé
BACKGROUND
BACKGROUND
Immune thrombocytopenia (ITP) is a common autoimmune disease characterized by loss of immune tolerance to platelet autoantigens leading to excessive destruction and insufficient production of platelets.
METHOD
METHODS
Quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed to detect the differentially expressed proteins in bone marrow samples from active ITP patients and normal controls.
RESULT
RESULTS
Our bioinformatic analysis identified two upregulated proteins (ORM1 and vWF) and two downregulated proteins (PPBP and SPARC) related to immune function. The four proteins were all found to be related to the tumor necrosis factor (TNF) -α signalling pathway and involved in the pathogenesis of ITP in KEGG pathway analysis.
CONCLUSION
CONCLUSIONS
Bioinformatics analysis identified differentially expressed proteins in bone marrow that are involved in the TNF-α signalling pathway and are related to the activation of immune function in ITP patients. These findings could provide new ideas for research on the loss of immune tolerance in ITP patients.
Identifiants
pubmed: 37355563
doi: 10.1186/s12014-023-09413-0
pii: 10.1186/s12014-023-09413-0
pmc: PMC10290381
doi:
Types de publication
Journal Article
Langues
eng
Pagination
24Subventions
Organisme : Taishan Youth Scholar Foundation of Shandong Province
ID : tsqn201812140
Organisme : Academic Promotion Program of Shandong First Medical University
ID : 2019RC018
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2021MH319
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2020MH112
Organisme : National Natural Science Foundation of China
ID : 81570104
Organisme : Key Research and Development Project of Jinan
ID : 201907021
Organisme : Projects of medical and health technology development program of Shandong province
ID : 2019WS498
Informations de copyright
© 2023. The Author(s).
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