A PPIX-binding probe facilitates discovery of PPIX-induced cell death modulation by peroxiredoxin.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
24 06 2023
24 06 2023
Historique:
received:
30
09
2022
accepted:
07
06
2023
medline:
26
6
2023
pubmed:
25
6
2023
entrez:
24
6
2023
Statut:
epublish
Résumé
While heme synthesis requires the formation of a potentially lethal intermediate, protoporphyrin IX (PPIX), surprisingly little is known about the mechanism of its toxicity, aside from its phototoxicity. The cellular protein interactions of PPIX might provide insight into modulators of PPIX-induced cell death. Here we report the development of PPB, a biotin-conjugated, PPIX-probe that captures proteins capable of interacting with PPIX. Quantitative proteomics in a diverse panel of mammalian cell lines reveal a high degree of concordance for PPB-interacting proteins identified for each cell line. Most differences are quantitative, despite marked differences in PPIX formation and sensitivity. Pathway and quantitative difference analysis indicate that iron and heme metabolism proteins are prominent among PPB-bound proteins in fibroblasts, which undergo PPIX-mediated death determined to occur through ferroptosis. PPB proteomic data (available at PRIDE ProteomeXchange # PXD042631) reveal that redox proteins from PRDX family of glutathione peroxidases interact with PPIX. Targeted gene knockdown of the mitochondrial PRDX3, but not PRDX1 or 2, enhance PPIX-induced death in fibroblasts, an effect blocked by the radical-trapping antioxidant, ferrostatin-1. Increased PPIX formation and death was also observed in a T-lymphoblastoid ferrochelatase-deficient leukemia cell line, suggesting that PPIX elevation might serve as a potential strategy for killing certain leukemias.
Identifiants
pubmed: 37355765
doi: 10.1038/s42003-023-05024-5
pii: 10.1038/s42003-023-05024-5
pmc: PMC10290680
doi:
Substances chimiques
Aminolevulinic Acid
88755TAZ87
protoporphyrin IX
C2K325S808
Peroxiredoxins
EC 1.11.1.15
Heme
42VZT0U6YR
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
673Subventions
Organisme : NCI NIH HHS
ID : P30 CA021765
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA096832
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA194057
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA194206
Pays : United States
Informations de copyright
© 2023. The Author(s).
Références
Sci Rep. 2016 Nov 18;6:36478
pubmed: 27857072
Cell Rep. 2014 Apr 10;7(1):180-93
pubmed: 24685134
J Biol Chem. 2012 Feb 10;287(7):4403-10
pubmed: 22147704
Int J Nanomedicine. 2013;8:2239-46
pubmed: 23818780
Curr Protoc Toxicol. 2001 May;Chapter 8:Unit 8.7
pubmed: 23045064
Genomics Proteomics Bioinformatics. 2003 Feb;1(1):78-86
pubmed: 15626337
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12339-44
pubmed: 12218177
Cell Physiol Biochem. 2014;34(6):1835-48
pubmed: 25502879
IUBMB Life. 2007 Aug-Sep;59(8-9):542-51
pubmed: 17701549
Pathobiology. 2009;76(6):303-14
pubmed: 19955842
Blood. 1997 Jan 1;89(1):1-25
pubmed: 8978272
J Pharmacol Exp Ther. 2016 Feb;356(2):267-75
pubmed: 26588930
J Biol Chem. 2004 Jun 4;279(23):24218-25
pubmed: 15044468
Pharmacol Ther. 2007 Jun;114(3):345-58
pubmed: 17368550
Photochem Photobiol. 1995 Jul;62(1):114-22
pubmed: 7638255
Life Sci. 2021 Jan 15;265:118862
pubmed: 33301812
Int J Mol Sci. 2019 Sep 07;20(18):
pubmed: 31500275
Oxid Med Cell Longev. 2019 Nov 11;2019:9613090
pubmed: 31827713
PLoS One. 2021 Apr 7;16(4):e0249650
pubmed: 33826667
Br J Cancer. 1996 Jul;74(1):22-9
pubmed: 8679453
Cell Commun Signal. 2015 Apr 10;13:24
pubmed: 25889964
Front Mol Biosci. 2018 Nov 19;5:97
pubmed: 30510932
Photochem Photobiol Sci. 2006 Aug;5(8):727-34
pubmed: 16886087
Biomed Opt Express. 2019 Apr 18;10(5):2478-2492
pubmed: 31149380
Indian J Biochem Biophys. 2005 Feb;42(1):7-12
pubmed: 23923575
Blood Rev. 2009 May;23(3):95-104
pubmed: 18835072
Br J Cancer. 2011 Mar 1;104(5):798-807
pubmed: 21304523
Cells. 2020 Oct 09;9(10):
pubmed: 33050207
Int J Mol Sci. 2021 Sep 19;22(18):
pubmed: 34576284
Cell Physiol Biochem. 2014;33(4):1186-96
pubmed: 24752138
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 15;193:23-32
pubmed: 29212045
Int J Oncol. 2008 Apr;32(4):861-7
pubmed: 18360713
Theranostics. 2018 Mar 8;8(8):2134-2146
pubmed: 29721068
J Biomed Opt. 2009 Jan-Feb;14(1):014007
pubmed: 19256695
Nat Cell Biol. 2014 Dec;16(12):1180-91
pubmed: 25402683
Biochim Biophys Acta. 2012 Sep;1823(9):1604-16
pubmed: 22554985
Redox Biol. 2020 Feb;30:101417
pubmed: 31901729
Sci Rep. 2017 Mar 22;7:45035
pubmed: 28327657
Sci Rep. 2019 Jun 17;9(1):8666
pubmed: 31209282
Toxicol In Vitro. 2007 Dec;21(8):1581-91
pubmed: 17637504
Immunol Ser. 1989;46:671-85
pubmed: 2488874
Toxicol Res (Camb). 2018 Jun 1;7(4):647-663
pubmed: 30090612
Int J Nanomedicine. 2017 May 31;12:4117-4127
pubmed: 28615940
Cell Death Dis. 2018 Jul 9;9(7):753
pubmed: 29988039
Molecules. 2016 Nov 17;21(11):
pubmed: 27869697
Biochim Biophys Acta. 2012 Mar;1820(3):264-81
pubmed: 21968002
Proc Natl Acad Sci U S A. 2002 May 14;99(10):6649-54
pubmed: 12011429
Hum Mol Genet. 2006 Mar 15;15(6):953-64
pubmed: 16467350
Blood. 2010 Jan 28;115(4):860-9
pubmed: 19965627
Front Oncol. 2020 Jun 17;10:965
pubmed: 32626657
Front Pharmacol. 2014 Aug 25;5:194
pubmed: 25202274
Cell Death Discov. 2019 Mar 11;5:77
pubmed: 30886745
Science. 2012 Jul 13;337(6091):195-9
pubmed: 22678362
Sci Rep. 2016 May 06;6:25353
pubmed: 27150264
J Exp Med. 2005 Oct 3;202(7):955-65
pubmed: 16203866
J Photochem Photobiol B. 2009 Sep 4;96(3):249-54
pubmed: 19646894
FASEB J. 2008 Feb;22(2):500-9
pubmed: 17875605
BMC Bioinformatics. 2008 Dec 29;9:559
pubmed: 19114008
PLoS One. 2018 Aug 31;13(8):e0202349
pubmed: 30169536
Mol Cell Proteomics. 2015 Jul;14(7):1898-910
pubmed: 25931508
JCI Insight. 2017 Aug 3;2(15):
pubmed: 28768907
FEBS Lett. 2013 Oct 1;587(19):3202-9
pubmed: 23954234
PLoS One. 2008 Aug 27;3(8):e3070
pubmed: 18728780
Mol Aspects Med. 2013 Apr-Jun;34(2-3):485-93
pubmed: 23506884
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15649-54
pubmed: 12429862
Inorg Chem. 2013 Nov 4;52(21):12223-33
pubmed: 24102308
Br J Cancer. 1999 May;80(5-6):676-84
pubmed: 10360643
Biochem J. 2009 Jun 12;421(1):51-8
pubmed: 19356151
Blood. 1994 Mar 15;83(6):1662-7
pubmed: 8123858
J Photochem Photobiol B. 2006 Sep 1;84(3):213-20
pubmed: 16709459
Onco Targets Ther. 2020 Jun 11;13:5429-5441
pubmed: 32606760
Curr Protoc Hum Genet. 2015 Jul 01;86:17.20.1-17.20.26
pubmed: 26132003
Anal Chem. 2003 Apr 15;75(8):1895-904
pubmed: 12713048
Comp Biochem Physiol C Toxicol Pharmacol. 2000 Jul;126(3):245-52
pubmed: 11048674
Biophys Chem. 2002 Apr 10;96(1):77-87
pubmed: 11975994
Mol Cells. 2016 Jan;39(1):26-30
pubmed: 26813658
Oncotarget. 2015 Aug 28;6(25):20742-3
pubmed: 26369700
Br J Cancer. 2001 Jul 20;85(2):279-84
pubmed: 11461090
Cell. 2012 May 25;149(5):1060-72
pubmed: 22632970
Br J Haematol. 2017 Feb;176(4):527-538
pubmed: 27982422
J Neurosci. 2012 Apr 25;32(17):5728-36
pubmed: 22539835