Role of DevR phosphorylation in nitric oxide homeostasis and signaling of Streptomyces coelicolor A3(2) M145.

Phosphorylation Streptomyces coelicolor / genetics Nitric Oxide / metabolism Bacterial Proteins / genetics Sequence Alignment Gene Expression Regulation, Bacterial
actinomycete nitric oxide phosphorylation signal transduction two-component system

Journal

FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721

Informations de publication

Date de publication:
17 01 2023
Historique:
received: 23 12 2022
revised: 09 06 2023
accepted: 18 06 2023
medline: 21 7 2023
pubmed: 25 6 2023
entrez: 25 6 2023
Statut: ppublish

Résumé

Our previous studies revealed that a two-component system (TCS), DevS, and DevR, regulate both nitric oxide (NO) signaling and NO homeostasis in the actinobacterium Streptomyces coelicolor A3(2) M145, suggesting a reasonable system for NO-dependent metabolism. In this study, sequence alignment of DevR and DevR homologs found Asp66 (D66) and Thr196 (T196) as predicted phosphorylation sites of DevR. Phos-tag gel electrophoretic mobility shift assay suggested that D66 and T196 are involved in the phosphorylation of DevR. The respective point mutations of D66 and T196 significantly decreased the transcriptional activity of DevR, which affected nitrite production and aerial mycelium formation. These results suggested that both D66 and T196 of DevR are important for the regulation of NO homeostasis and signaling in S. coelicolor A3(2) M145.

Identifiants

DOI: 10.1093/femsle/fnad060 PMID: 37355778
pubmed: 37355778
pii: 7207394
doi: 10.1093/femsle/fnad060
pii:
doi:

Substances chimiques

Nitric Oxide 31C4KY9ESH
Bacterial Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.

Auteurs

Sota Honma (S)

Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 1568502, Japan.

Shinsaku Ito (S)

Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 1568502, Japan.

Shunsuke Yajima (S)

Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 1568502, Japan.

Yasuyuki Sasaki (Y)

Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 1568502, Japan.
ORCID: 0000-0002-6274-8052

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Phosphorylation › Role of DevR phosphorylation in nitric oxide...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Streptomyces coelicolor › Role of DevR phosphorylation in nitric oxide...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Oxydes d'azote › Monoxyde d'azote › Role of DevR phosphorylation in nitric oxide...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Role of DevR phosphorylation in nitric oxide...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › Role of DevR phosphorylation in nitric oxide...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Role of DevR phosphorylation in nitric oxide...