Human 8-cell embryo-like cells from pluripotent stem cells.
Journal
Current opinion in genetics & development
ISSN: 1879-0380
Titre abrégé: Curr Opin Genet Dev
Pays: England
ID NLM: 9111375
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
18
01
2023
revised:
28
04
2023
accepted:
24
05
2023
medline:
15
8
2023
pubmed:
26
6
2023
entrez:
25
6
2023
Statut:
ppublish
Résumé
The totipotent embryo initiates transcription during zygotic or embryonic genome activation (EGA, ZGA). ZGA occurs at the 8-cell stage in humans and its failure leads to developmental arrest. Understanding the molecular pathways underlying ZGA and totipotency is essential to comprehend human development. Recently, human 8-cell-like cells (8CLCs) have been discovered in vitro that resemble the 8-cell embryo. 8CLCs exist among naive pluripotent stem cells and can be induced genetically or chemically. Their ZGA-like transcriptome, transposable element activation, 8-cell embryo-specific protein expression, and developmental properties make them an exceptional model system to study early embryonic cell-state transitions and human totipotency programs in vitro.
Identifiants
pubmed: 37356343
pii: S0959-437X(23)00046-1
doi: 10.1016/j.gde.2023.102066
pii:
doi:
Substances chimiques
DUX4L1 protein, human
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
102066Subventions
Organisme : Wellcome Trust
ID : 210754/Z/18/Z
Pays : United Kingdom
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest W.R. is a consultant and shareholder of Cambridge Epigenetix. J.T.S. and W.R. are employees of Altos Labs.