Phylogenetic analysis and functional characterization of norcoclaurine synthase involved in benzylisoquinoline alkaloids biosynthesis in Stephania tetrandra.
Menispermaceae
Pictet-Spengler condensation
benzylisoquinoline alkaloids
norcoclaurine synthase
phylogenetic analysis
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
25 Jun 2023
25 Jun 2023
Historique:
revised:
03
06
2023
received:
21
04
2023
accepted:
08
06
2023
medline:
26
6
2023
pubmed:
26
6
2023
entrez:
26
6
2023
Statut:
aheadofprint
Résumé
Benzylisoquinoline alkaloids (BIAs) are a class of secondary metabolites that possess diverse pharmaceutical properties and are exclusively accumulated in specific plant genera. The Pictet-Spengler condensation, catalyzed by norcoclaurine synthase (NCS), represents a key enzymatic reaction in the biosynthetic pathway of BIAs. While NCS genes have been identified in several plant families such as Papaveraceae, Berberidaceae, and Ranunculaceae, no NCS genes have been reported in Menispermaceae, which is another genus known to accumulate BIAs. Here, NCSs were isolated and functionally characterized from the Menispermaceae family plant Stephania tetrandra. In vitro enzyme assay identified two functional StNCSs which could catalyze the formation of (S)-norcoclaurine. These functionally characterized genes were then integrated into engineered yeast to enable the production of norcoclaurine. Phylogenetic analysis of the NCS enzymes revealed that the StNCSs predominantly clustered into two clades. The functional StNCSs clustered with known NCSs, highlighting the presence of a specific NCS catalytic domain. This study not only provides additional genetic components for the synthetic biology-based production of BIAs in yeast but also contributes to the understanding of the phylogenetic relationships and structure-function relationship of NCS genes involved in the origin and production of BIAs.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Key R&D Program of China
ID : 2020YFA0908000
Organisme : National Natural Science Foundation of China
ID : 82011530137
Organisme : National Natural Science Foundation of China
ID : 31961133007
Organisme : Key Project at Central Government Level: The Ability to Establish Sustainable Use of Valuable Chinese Medicine Resources
ID : 2060302
Organisme : Fundamental Research Funds of China Academy of Chinese Medical Sciences
ID : ZZ13-YQ-083
Organisme : Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine
ID : ZYYCXTD-D-202005
Organisme : Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences
ID : CI2021B014
Informations de copyright
© 2023 Wiley Periodicals LLC.
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