Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.
chromosomes
cohesin
condensin
mitosis
sister chromatid resolution
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
15 08 2023
15 08 2023
Historique:
revised:
26
05
2023
received:
10
01
2023
accepted:
14
06
2023
medline:
16
8
2023
pubmed:
26
6
2023
entrez:
26
6
2023
Statut:
ppublish
Résumé
Genetic information is stored in linear DNA molecules, which are highly folded inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in an intertwined arrangement. Dividing cells must disentangle and condense the sister chromatids into separate bodies such that a microtubule-based spindle can move them to opposite poles. While the spindle-mediated transport of sister chromatids has been studied in detail, the chromosome-intrinsic mechanics presegregating sister chromatids have remained elusive. Here, we show that human sister chromatids resolve extensively already during interphase, in a process dependent on the loop-extruding activity of cohesin, but not that of condensins. Increasing cohesin's looping capability increases sister DNA resolution in interphase nuclei to an extent normally seen only during mitosis, despite the presence of abundant arm cohesion. That cohesin can resolve sister chromatids so extensively in the absence of mitosis-specific activities indicates that DNA loop extrusion is a generic mechanism for segregating replicated genomes, shared across different Structural Maintenance of Chromosomes (SMC) protein complexes in all kingdoms of life.
Identifiants
pubmed: 37357575
doi: 10.15252/embj.2023113475
pmc: PMC10425840
doi:
Substances chimiques
Chromosomal Proteins, Non-Histone
0
Cell Cycle Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e113475Subventions
Organisme : Austrian Science Fund FWF
ID : DK W1238
Pays : Austria
Organisme : European Research Council
ID : 101019039
Pays : International
Organisme : European Research Council
ID : 693949
Pays : International
Informations de copyright
© 2023 The Authors. Published under the terms of the CC BY 4.0 license.
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