Bezafibrate reduces the damage, activation and mechanical properties of lung fibroblast cells induced by hydrogen peroxide.

Humans Hydrogen Peroxide / toxicity Reactive Oxygen Species / metabolism Bezafibrate / pharmacology Pulmonary Fibrosis / pathology Lung / metabolism Oxidative Stress Fibroblasts RNA, Messenger / metabolism
Bezafibrate Cell membrane elasticity Lung fibrosis MRC-5 cells Oxidative damage

Journal

Naunyn-Schmiedeberg's archives of pharmacology
ISSN: 1432-1912
Titre abrégé: Naunyn Schmiedebergs Arch Pharmacol
Pays: Germany
ID NLM: 0326264

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 18 01 2023
accepted: 20 06 2023
medline: 14 11 2023
pubmed: 26 6 2023
entrez: 26 6 2023
Statut: ppublish

Résumé

In pulmonary fibrosis, the proliferation of fibroblasts and their differentiation into myofibroblasts is often caused by tissue damage, such as oxidative damage caused by reactive oxygen species, which leads to progressive rupture and thus destruction of the alveolar architecture, resulting in cell proliferation and tissue remodeling. Bezafibrate (BZF) is an important member of the peroxisome proliferator-activated receptor (PPARs) family agonists, used in clinical practice as antihyperlipidemic. However, the antifibrotic effects of BZF are still poorly studied. The objective of this study was to evaluate the effects of BZF on pulmonary oxidative damage in lung fibroblast cells. MRC-5 cells were treated with hydrogen peroxide (H

Identifiants

DOI: 10.1007/s00210-023-02595-2 PMID: 37358795
pubmed: 37358795
doi: 10.1007/s00210-023-02595-2
pii: 10.1007/s00210-023-02595-2
doi:

Substances chimiques

Hydrogen Peroxide BBX060AN9V
Reactive Oxygen Species 0
Bezafibrate Y9449Q51XH
RNA, Messenger 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3857-3866

Subventions

Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior , Brasil
ID : Financial Code 001
Organisme : National Council for Scientific and Technological Development
ID : Grant#311424/2018-0

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Références

Aebi H (1984) Catalase in vitro. In: Methods in enzymology. pp 121–126
Aguilar Diaz De Leon J, Borges CR (2020) Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay. J Vis Exp. https://doi.org/10.3791/61122
Avouac J, Konstantinova I, Guignabert C et al (2017) Pan-PPAR agonist IVA337 is effective in experimental lung fibrosis and pulmonary hypertension. Ann Rheum Dis 76:1931–1940. https://doi.org/10.1136/annrheumdis-2016-210821
doi: 10.1136/annrheumdis-2016-210821 pubmed: 28801346
Awonuga AO, Chatzicharalampous C, Thakur M et al (2022) Genetic and epidemiological similarities, and differences between postoperative intraperitoneal adhesion development and other benign fibro-proliferative disorders. Reprod Sci 29:3055–3077. https://doi.org/10.1007/s43032-021-00726-9
doi: 10.1007/s43032-021-00726-9 pubmed: 34515982
Cameli P, Carleo A, Bergantini L et al (2020) Oxidant/antioxidant disequilibrium in idiopathic pulmonary fibrosis pathogenesis. Inflammation 43:1–7. https://doi.org/10.1007/s10753-019-01059-1
doi: 10.1007/s10753-019-01059-1 pubmed: 31297749
Cannavò L, Perrone S, Viola V et al (2021) Oxidative stress and respiratory diseases in preterm newborns. Int J Mol Sci 22:12504. https://doi.org/10.3390/ijms222212504
doi: 10.3390/ijms222212504 pubmed: 34830385 pmcid: 8625766
Cheresh P, Kim S-J, Tulasiram S, Kamp DW (2013) Oxidative stress and pulmonary fibrosis. Biochim Biophys Acta - Mol Basis Dis 1832:1028–1040. https://doi.org/10.1016/j.bbadis.2012.11.021
doi: 10.1016/j.bbadis.2012.11.021
Cuenot S, Bouchara J-P (2018) Atomic force microscopy: a promising tool for deciphering the pathogenic mechanisms of fungi in cystic fibrosis. Mycopathologia 183:291–310. https://doi.org/10.1007/s11046-017-0201-1
doi: 10.1007/s11046-017-0201-1 pubmed: 29128932
Ding Y, Xu G-K, Wang G-F (2017) On the determination of elastic moduli of cells by AFM based indentation. Sci Rep 7:45575. https://doi.org/10.1038/srep45575
doi: 10.1038/srep45575 pubmed: 28368053 pmcid: 5377332
Dong H, Yang J, Wang Y et al (2020) Polysaccharide SAFP from Sarcodon aspratus attenuates oxidative stress-induced cell damage and bleomycin-induced pulmonary fibrosis. Int J Biol Macromol 164:1215–1236. https://doi.org/10.1016/j.ijbiomac.2020.07.120
doi: 10.1016/j.ijbiomac.2020.07.120 pubmed: 32693133
Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid Peroxidation. Methods Enzymol 186:421–431. https://doi.org/10.1016/0076-6879(90)86135-I
doi: 10.1016/0076-6879(90)86135-I pubmed: 2233309
Duckworth A, Gibbons MA, Allen RJ et al (2021) Telomere length and risk of idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease: a mendelian randomisation study. Lancet Respir Med 9:285–294. https://doi.org/10.1016/S2213-2600(20)30364-7
doi: 10.1016/S2213-2600(20)30364-7 pubmed: 33197388
Dulínska-Molak I, Mao H, Kawazoe N, Chen G (2014) Variation of mechanical property of single-walled carbon nanotubes-treated cells explored by atomic force microscopy. J Biomed Nanotechnol 10:651–659. https://doi.org/10.1166/jbn.2014.1745
doi: 10.1166/jbn.2014.1745 pubmed: 24734517
Francis LW, Gonzalez D, Ryder T et al (2010) Optimized sample preparation for high-resolution AFM characterization of fixed human cells. J Microsc 240:111–121. https://doi.org/10.1111/j.1365-2818.2010.03392.x
doi: 10.1111/j.1365-2818.2010.03392.x pubmed: 20946377
Glass DS, Grossfeld D, Renna HA et al (2022) Idiopathic pulmonary fibrosis: Current and future treatment. Clin Respir J 16:84–96. https://doi.org/10.1111/crj.13466
doi: 10.1111/crj.13466 pubmed: 35001525 pmcid: 9060042
Glorieux C, Calderon PB (2017) Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem 398:1095–1108. https://doi.org/10.1515/hsz-2017-0131
doi: 10.1515/hsz-2017-0131 pubmed: 28384098
Guz N, Dokukin M, Kalaparthi V, Sokolov I (2014) If cell mechanics can be described by elastic modulus: study of different models and probes used in indentation experiments. Biophys J 107:564–575. https://doi.org/10.1016/j.bpj.2014.06.033
doi: 10.1016/j.bpj.2014.06.033 pubmed: 25099796 pmcid: 4129501
Han X, Wu Y, Yang Q, Cao G (2021) Peroxisome proliferator-activated receptors in the pathogenesis and therapies of liver fibrosis. Pharmacol Ther 222:107791. https://doi.org/10.1016/j.pharmthera.2020.107791
doi: 10.1016/j.pharmthera.2020.107791 pubmed: 33321113
Hu X, Xu Q, Wan H et al (2020) PI3K-Akt-mTOR/PFKFB3 pathway mediated lung fibroblast aerobic glycolysis and collagen synthesis in lipopolysaccharide-induced pulmonary fibrosis. Lab Investig 100:801–811. https://doi.org/10.1038/s41374-020-0404-9
doi: 10.1038/s41374-020-0404-9 pubmed: 32051533
Ibrahim SA, Mohamed MZ, El-Tahawy NF, Abdelrahman AM (2021) Antifibrotic effects of bezafibrate and pioglitazone against thioacetamide-induced liver fibrosis in albino rats. Can J Physiol Pharmacol 99:313–320. https://doi.org/10.1139/cjpp-2020-0159
doi: 10.1139/cjpp-2020-0159 pubmed: 32721217
Illner D, Scherthan H (2013) Ionizing irradiation-induced radical stress stalls live meiotic chromosome movements by altering the actin cytoskeleton. Proc Natl Acad Sci 110:16027–16032. https://doi.org/10.1073/pnas.1306324110
doi: 10.1073/pnas.1306324110 pubmed: 24046368 pmcid: 3791724
Júnior C, Narciso M, Marhuenda E et al (2021) Baseline stiffness modulates the non-linear response to stretch of the extracellular matrix in pulmonary fibrosis. Int J Mol Sci 22:12928. https://doi.org/10.3390/ijms222312928
doi: 10.3390/ijms222312928 pubmed: 34884731 pmcid: 8657558
Junn E, Lee KN, Ju HR et al (2000) Requirement of hydrogen peroxide generation in TGF-β1 signal transduction in human lung fibroblast cells: involvement of hydrogen peroxide and Ca 2+ in TGF-β1-induced IL-6 expression. J Immunol 165:2190–2197. https://doi.org/10.4049/jimmunol.165.4.2190
doi: 10.4049/jimmunol.165.4.2190 pubmed: 10925306
Kim HJ, Perlman D, Tomic R (2015) Natural history of idiopathic pulmonary fibrosis. Respir Med 109:661–670. https://doi.org/10.1016/j.rmed.2015.02.002
doi: 10.1016/j.rmed.2015.02.002 pubmed: 25727856
King TE, Pardo A, Selman M (2011) Idiopathic pulmonary fibrosis. Lancet 378:1949–1961. https://doi.org/10.1016/S0140-6736(11)60052-4
doi: 10.1016/S0140-6736(11)60052-4 pubmed: 21719092
Kishaba T (2019) Acute exacerbation of idiopathic pulmonary fibrosis. Medicina (B Aires) 55:70. https://doi.org/10.3390/medicina55030070
doi: 10.3390/medicina55030070
Kuznetsova TG, Starodubtseva MN, Yegorenkov NI et al (2007) Atomic force microscopy probing of cell elasticity. Micron 38:824–833. https://doi.org/10.1016/j.micron.2007.06.011
doi: 10.1016/j.micron.2007.06.011 pubmed: 17709250
Li S, Shi J, Tang H (2022) Animal models of drug-induced pulmonary fibrosis: an overview of molecular mechanisms and characteristics. Cell Biol Toxicol 38:699–723. https://doi.org/10.1007/s10565-021-09676-z
doi: 10.1007/s10565-021-09676-z pubmed: 34741237
Liu X, Liu H, Zhai Y et al (2017) Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2. PeerJ 5:e3642. https://doi.org/10.7717/peerj.3642
doi: 10.7717/peerj.3642 pubmed: 28785522 pmcid: 5541921
Lund M, Andersen KG, Heaton R et al (2021) Bezafibrate activation of PPAR drives disturbances in mitochondrial redox bioenergetics and decreases the viability of cells from patients with VLCAD deficiency. Biochim Biophys Acta - Mol Basis Dis 1867:166100. https://doi.org/10.1016/j.bbadis.2021.166100
doi: 10.1016/j.bbadis.2021.166100 pubmed: 33549744
Lv Q, Wang J, Xu C et al (2020) Pirfenidone alleviates pulmonary fibrosis in vitro and in vivo through regulating Wnt/GSK-3β/β-catenin and TGF-β1/Smad2/3 signaling pathways. Mol Med 26:49. https://doi.org/10.1186/s10020-020-00173-3
doi: 10.1186/s10020-020-00173-3 pubmed: 32448163 pmcid: 7245944
Mandras SA, Mehta HS, Vaidya A (2020) Pulmonary hypertension: a brief guide for clinicians. Mayo Clin Proc 95:1978–1988. https://doi.org/10.1016/j.mayocp.2020.04.039
doi: 10.1016/j.mayocp.2020.04.039 pubmed: 32861339
Martinez FJ, Collard HR, Pardo A et al (2017) Idiopathic pulmonary fibrosis. Nat Rev Dis Prim 3:17074. https://doi.org/10.1038/nrdp.2017.74
doi: 10.1038/nrdp.2017.74 pubmed: 29052582
Piotrowski W, Martusewicz-Boros M, Białas A et al (2022) Guidelines of the polish respiratory society on the diagnosis and treatment of progressive fibrosing interstitial lung diseases other than idiopathic pulmonary fibrosis. Adv Respir Med 90:425–450. https://doi.org/10.3390/arm90050052
doi: 10.3390/arm90050052 pubmed: 36285980 pmcid: 9717335
Poch D, Mandel J (2021) Pulmonary hypertension. Ann Intern Med 174:ITC49–ITC64. https://doi.org/10.7326/AITC202104200
doi: 10.7326/AITC202104200 pubmed: 33844574
Que X, Hung M-Y, Yeang C et al (2018) Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. Nature 558:301–306. https://doi.org/10.1038/s41586-018-0198-8
doi: 10.1038/s41586-018-0198-8 pubmed: 29875409 pmcid: 6033669
Roehlen N, Crouchet E, Baumert TF (2020) Liver fibrosis: mechanistic concepts and therapeutic perspectives. Cells 9:875. https://doi.org/10.3390/cells9040875
doi: 10.3390/cells9040875 pubmed: 32260126 pmcid: 7226751
Su H, Wan C, Song A, et al (2019a) Oxidative stress and renal fibrosis: mechanisms and therapies. pp 585–604
Su L-J, Zhang J-H, Gomez H et al (2019b) Reactive oxygen species-induced lipid peroxidation in apoptosis, autophagy, and ferroptosis. Oxid Med Cell Longev 2019:1–13. https://doi.org/10.1155/2019/5080843
doi: 10.1155/2019/5080843
Sun K-H, Chang Y, Reed NI, Sheppard D (2016) α-Smooth muscle actin is an inconsistent marker of fibroblasts responsible for force-dependent TGFβ activation or collagen production across multiple models of organ fibrosis. Am J Physiol Cell Mol Physiol 310:L824–L836. https://doi.org/10.1152/ajplung.00350.2015
doi: 10.1152/ajplung.00350.2015
Sunnetcioglu A, Alp HH, Sertogullar ndan B, et al (2016) Evaluation of oxidative damage and antioxidant mechanisms in COPD, Lung Cancer, and Obstructive Sleep Apnea Syndrome. Respir Care 61:205–211. https://doi.org/10.4187/respcare.04209
doi: 10.4187/respcare.04209 pubmed: 26604330
Tanaka K, Chamoto K, Saeki S et al (2022) Combination bezafibrate and nivolumab treatment of patients with advanced non–small cell lung cancer. Sci Transl Med 14:eabq0021. https://doi.org/10.1126/scitranslmed.abq0021
doi: 10.1126/scitranslmed.abq0021 pubmed: 36516270
Xia F, Youcef-Toumi K (2022) Review: advanced atomic force microscopy modes for biomedical research. Biosensors 12:1116. https://doi.org/10.3390/bios12121116
doi: 10.3390/bios12121116 pubmed: 36551083 pmcid: 9775674

Auteurs

Camille Kirinus Reghelin (CK)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Matheus Scherer Bastos (MS)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil. matheus.bastos@acad.pucrs.br.
Laboratório de Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), 6681 Ipiranga Ave., Porto Alegre, RS, Zip Code: 90619-900, Brazil. matheus.bastos@acad.pucrs.br.

Bruno de Souza Basso (B)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Bruna Pasqualotto Costa (BP)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Kelly Goulart Lima (KG)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Arieli Cruz de Sousa (AC)

Departamento de Bioquímica, ICBS, Universidade Federal Do Rio Grande Do Sul (UFRGS), Rua Ramiro Barcelos, 2600-Anexo I, Porto Alegre, RS, CEP 90035-003, Brazil.

Gabriela Viegas Haute (GV)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Fernando Mendonça Diz (FM)

Programa de Pós-Graduação Em Engenharia E Tecnologia de Materiais, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Henrique Bregolin Dias (HB)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Carolina Luft (C)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Kétlin Fernanda Rodrigues (KF)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Maria Cláudia Rosa Garcia (MCR)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Lucas Strassburger Matzenbacher (LS)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Bruno Silveira Adami (BS)

Laboratório Central de Microscopia E Microanálise (LabCEMM), Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Léder Leal Xavier (LL)

Laboratório Central de Microscopia E Microanálise (LabCEMM), Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Márcio Vinícius Fagundes Donadio (MVF)

Laboratório de Atividade Física Pediátrica, Centro Infantil, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Jarbas Rodrigues de Oliveira (JR)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

Denizar Alberto da Silva Melo (DA)

Laboratório de Pesquisa Em Biofísica Celular E Inflamação, Pontifícia Universidade Católica Do Rio Grande Do Sul (PUCRS), Porto Alegre, RS, Brazil.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Espèces réactives de l'oxygène › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Éthers phényliques › Acides fibriques › Bézafibrate › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Fibrose › Fibrose pulmonaire › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Appareil respiratoire › Poumon › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress oxydatif › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Bezafibrate reduces the damage, activation and...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Bezafibrate reduces the damage, activation and...