Inaccuracies in plasma oxytocin extraction and enzyme immunoassay techniques.
Enzyme-linked immunosorbent assay
Oxytocin
Reverse-phase chromatography
Solid phase extraction
Journal
Comprehensive psychoneuroendocrinology
ISSN: 2666-4976
Titre abrégé: Compr Psychoneuroendocrinol
Pays: England
ID NLM: 101774169
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
16
08
2022
revised:
01
06
2023
accepted:
04
06
2023
medline:
26
6
2023
pubmed:
26
6
2023
entrez:
26
6
2023
Statut:
epublish
Résumé
Numerous studies have reported extensive associations between plasma oxytocin (OXT) concentrations and various human physiological and neurobehavioral processes. Measurement of OXT is fraught with difficulty due to its low molecular weight and plasma concentrations, with no consensus as to the optimal conditions for pre-analytical sample extraction, standards for immunoassay validation or the ideal protease inhibitors to prevent OXT degradation. Previous attempts at determining the efficacy of various purification techniques such as solid phase extraction (SPE) or ultrafiltration have only utilized human plasma samples, making it difficult to dissect out whether the effect of interference comes from the extraction process itself or cross-reactivity with other proteins. By testing these on pure OXT solutions, we demonstrate poor recovery efficacy and reliability of reversed phase SPE (maximum 58.1%) and ultrafiltration (<1%) techniques, and the potential for the former to introduce interference into enzyme immunoassay (EIA) measurements. The clonality of antibodies used in EIA kits also potentially contributes to the differences in the readings obtained, and we validate an EIA kit which did not require pre-analytical sample extraction with low cross-reactivity and high reliability (intraclass correlation coefficient 0.980 (95% CI 0.896-0.999). Biochemical techniques used for measuring plasma OXT concentrations must therefore be internally validated prior to translation into clinical studies.
Identifiants
pubmed: 37360277
doi: 10.1016/j.cpnec.2023.100188
pii: S2666-4976(23)00022-X
pmc: PMC10285453
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100188Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
None.
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