Coumarin 6 staining method to detect microplastics.

Microplastics Plastics Water Pollutants, Chemical / analysis Environmental Monitoring Staining and Labeling
Coumarin Detection Fluorescence Microplastic Staining

Journal

Marine pollution bulletin
ISSN: 1879-3363
Titre abrégé: Mar Pollut Bull
Pays: England
ID NLM: 0260231

Informations de publication

Date de publication:
Aug 2023
Historique:
received: 07 04 2023
revised: 07 06 2023
accepted: 09 06 2023
medline: 26 7 2023
pubmed: 26 6 2023
entrez: 26 6 2023
Statut: ppublish

Résumé

Microplastics have contaminated the ocean in large quantities and are widely distributed throughout the world. Thus, our understanding of the concentration of microplastics in various environments should be increased. However, current methods to detect microplastics require considerable effort and expensive equipment. In this study, we developed a fluorescence staining technique using coumarin 6 and examined its effectiveness. A mixture of acetone and ethanol was used as the solvent, and 10 different types of plastics were able to be stained with coumarin 6. The fluorescence peak for coumarin 6 staining was approximately 500 nm for each plastic type. The optimal immersion time and coumarin 6 concentration for staining were determined to be 60 min and 1 mg L

Identifiants

DOI: 10.1016/j.marpolbul.2023.115167 PMID: 37364336
pubmed: 37364336
pii: S0025-326X(23)00600-8
doi: 10.1016/j.marpolbul.2023.115167
pii:
doi:

Substances chimiques

Microplastics 0
Plastics 0
coumarin 6 0
Water Pollutants, Chemical 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

115167

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Yipeng Cheng (Y)

Tokyo University of Marine Science and Technology, 5-7, Konan-4, Minato, Tokyo 108-8477, Japan.

Jiaqi Zhang (J)

Tokyo University of Marine Science and Technology, 5-7, Konan-4, Minato, Tokyo 108-8477, Japan. Electronic address: m223076@edu.kaiyodai.ac.jp.

Haruka Nakano (H)

Tokyo University of Marine Science and Technology, 5-7, Konan-4, Minato, Tokyo 108-8477, Japan.

Naofumi Ueyama (N)

Tokyo University of Marine Science and Technology, 5-7, Konan-4, Minato, Tokyo 108-8477, Japan.

Hisayuki Arakawa (H)

Tokyo University of Marine Science and Technology, 5-7, Konan-4, Minato, Tokyo 108-8477, Japan. Electronic address: arakawa@kaiyodai.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Microplastiques › Coumarin 6 staining method to detect microplastics.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Coumarin 6 staining method to detect microplastics.
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Polluants environnementaux › Polluants de l'eau › Polluants chimiques de l'eau › Coumarin 6 staining method to detect microplastics.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Surveillance de l'environnement › Coumarin 6 staining method to detect microplastics.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Coumarin 6 staining method to detect microplastics.