Dissecting Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Murine Dendritic Cells.

Mice Animals Antigen Presentation Phagocytosis Phagosomes / metabolism Receptors, Pattern Recognition / metabolism Dendritic Cells
Antigen presentation Dendritic cells Eα MHC-II Phagocytosis

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
medline: 28 6 2023
pubmed: 27 6 2023
entrez: 26 6 2023
Statut: ppublish

Résumé

Phagosomal pattern recognition receptor signaling promotes phagosome maturation and additional immune pathways such as proinflammatory cytokine secretion and antigen MHC-II presentation in antigen-presenting cells. In the present chapter, we describe procedures to assess these pathways in murine dendritic cells, professional phagocytes positioned at the interface between innate and adaptive immune responses. The assays described herein follow proinflammatory signaling by biochemical and immunological assays as well as antigen presentation of the model antigen Eα by immunofluorescence followed by flow cytometry.

Identifiants

DOI: 10.1007/978-1-0716-3338-0_19 PMID: 37365475
pubmed: 37365475
doi: 10.1007/978-1-0716-3338-0_19
doi:

Substances chimiques

Receptors, Pattern Recognition 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

275-287

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI137173
Pays : United States

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Steinman RM, Hemmi H (2006) Dendritic cells: translating innate to adaptive immunity. Curr Top Microbiol Immunol 311:17–58
pubmed: 17048704
Janeway CA Jr, Medzhitov R (2002) Innate immune recognition. Annu Rev Immunol 20:197–216. https://doi.org/10.1146/annurev.immunol.20.083001.084359
doi: 10.1146/annurev.immunol.20.083001.084359 pubmed: 11861602
Kagan JC, Iwasaki A (2012) Phagosome as the organelle linking innate and adaptive immunity. Traffic 13(8):1053–1061. https://doi.org/10.1111/j.1600-0854.2012.01377.x
doi: 10.1111/j.1600-0854.2012.01377.x pubmed: 22577865 pmcid: 3658133
Kawai T, Akira S (2010) The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat Immunol 11(5):373–384
doi: 10.1038/ni.1863 pubmed: 20404851
Kumar H, Kawai T, Akira S (2011) Pathogen recognition by the innate immune system. Int Rev Immunol 30(1):16–34
doi: 10.3109/08830185.2010.529976 pubmed: 21235323
Villadangos JA, Schnorrer P, Wilson NS (2005) Control of MHC class II antigen presentation in dendritic cells: a balance between creative and destructive forces. Immunol Rev 207:191–205. https://doi.org/10.1111/j.0105-2896.2005.00317.x
doi: 10.1111/j.0105-2896.2005.00317.x pubmed: 16181337
Mantegazza AR, Magalhaes JG, Amigorena S, Marks MS (2013) Presentation of phagocytosed antigens by MHC class I and II. Traffic 14(2):135–152. https://doi.org/10.1111/tra.12026
doi: 10.1111/tra.12026 pubmed: 23127154
Cresswell P (1996) Invariant chain structure and MHC class II function. Cell 84(4):505–507. https://doi.org/10.1016/s0092-8674(00)81025-9
doi: 10.1016/s0092-8674(00)81025-9 pubmed: 8598037
Niedergang F, Grinstein S (2018) How to build a phagosome: new concepts for an old process. Curr Opin Cell Biol 50:57–63. https://doi.org/10.1016/j.ceb.2018.01.009
doi: 10.1016/j.ceb.2018.01.009 pubmed: 29471269
Savina A, Amigorena S (2007) Phagocytosis and antigen presentation in dendritic cells. Immunol Rev 219:143–156
doi: 10.1111/j.1600-065X.2007.00552.x pubmed: 17850487
Blander JM, Medzhitov R (2006) On regulation of phagosome maturation and antigen presentation. Nat Immunol 7(10):1029–1035. https://doi.org/10.1038/ni1006-1029
doi: 10.1038/ni1006-1029 pubmed: 16985500
Guermonprez P, Saveanu L, Kleijmeer M, Davoust J, Van Endert P, Amigorena S (2003) ER-phagosome fusion defines an MHC class I cross-presentation compartment in dendritic cells. Nature 425(6956):397–402. https://doi.org/10.1038/nature01911
doi: 10.1038/nature01911 pubmed: 14508489
Rudensky A, Rath S, Preston-Hurlburt P, Murphy DB, Janeway CA Jr (1991) On the complexity of self. Nature 353(6345):660–662. https://doi.org/10.1038/353660a0
doi: 10.1038/353660a0
Rudensky Y, Preston-Hurlburt P, Hong SC, Barlow A, Janeway CA Jr (1991) Sequence analysis of peptides bound to MHC class II molecules. Nature 353(6345):622–627. https://doi.org/10.1038/353622a0
doi: 10.1038/353622a0 pubmed: 1656276
Bishop GA (1998) H2 class II. Encyclopedia of immunology, 2nd edn, pp 1040–1045. https://doi.org/10.1006/rwei.1999.0270
doi: 10.1006/rwei.1999.0270
Lee HK, Mattei LM, Steinberg BE, Alberts P, Lee YH, Chervonsky A, Mizushima N, Grinstein S, Iwasaki A (2010) In vivo requirement for Atg5 in antigen presentation by dendritic cells. Immunity 32(2):227–239
doi: 10.1016/j.immuni.2009.12.006 pubmed: 20171125 pmcid: 2996467
Mantegazza AR, Guttentag SH, El-Benna J, Sasai M, Iwasaki A, Shen H, Laufer TM, Marks MS (2012) Adaptor protein-3 in dendritic cells facilitates phagosomal toll-like receptor signaling and antigen presentation to CD4(+) T cells. Immunity 36(5):782–794
doi: 10.1016/j.immuni.2012.02.018 pubmed: 22560444 pmcid: 3361531
Savina A, Peres A, Cebrian I, Carmo N, Moita C, Hacohen N, Moita LF, Amigorena S (2009) The small GTPase Rac2 controls phagosomal alkalinization and antigen crosspresentation selectively in CD8(+) dendritic cells. Immunity 30(4):544–555
doi: 10.1016/j.immuni.2009.01.013 pubmed: 19328020
Geem D, Medina-Contreras O, Kim W, Huang CS, Denning TL (2012) Isolation and characterization of dendritic cells and macrophages from the mouse intestine. J Vis Exp 63:e4040. https://doi.org/10.3791/4040
doi: 10.3791/4040
Winzler C, Rovere P, Rescigno M, Granucci F, Penna G, Adorini L, Zimmermann VS, Davoust J, Ricciardi-Castagnoli P (1997) Maturation stages of mouse dendritic cells in growth factor-dependent long-term cultures. J Exp Med 185(2):317–328
doi: 10.1084/jem.185.2.317 pubmed: 9016880 pmcid: 2196118
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685. https://doi.org/10.1038/227680a0
doi: 10.1038/227680a0 pubmed: 5432063
Savina A, Vargas P, Guermonprez P, Lennon AM, Amigorena S (2010) Measuring pH, ROS production, maturation, and degradation in dendritic cell phagosomes using cytofluorometry-based assays. Methods Mol Biol 595:383–402. https://doi.org/10.1007/978-1-60761-421-0_25
doi: 10.1007/978-1-60761-421-0_25 pubmed: 19941126
Sasai M, Linehan MM, Iwasaki A (2010) Bifurcation of Toll-like receptor 9 signaling by adaptor protein 3. Science 329(5998):1530–1534. https://doi.org/10.1126/science.1187029
doi: 10.1126/science.1187029 pubmed: 20847273 pmcid: 3063333

Auteurs

Emilia Scharrig (E)

Department of Microbiology and Immunology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.

Adriana R Mantegazza (AR)

Department of Microbiology and Immunology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA. adriana.mantegazza@jefferson.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Dissecting Phagosomal Pattern Recognition...
questionsmedicales.fr › Eucaryotes › Animaux › Dissecting Phagosomal Pattern Recognition...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité acquise › Immunité cellulaire › Présentation d'antigène › Dissecting Phagosomal Pattern Recognition...
questionsmedicales.fr › Phénomènes du système immunitaire › Phagocytose › Dissecting Phagosomal Pattern Recognition...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Phagosomes › Dissecting Phagosomal Pattern Recognition...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs de reconnaissance de motifs moléculaires › Dissecting Phagosomal Pattern Recognition...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système phagocytaire mononucléé › Cellules dendritiques › Dissecting Phagosomal Pattern Recognition...