A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease.
Cryopreservation
LN
Lymphoid tissue fibroblasts
Stromal cells
Tonsil
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
revised:
03
05
2023
received:
29
12
2022
accepted:
19
05
2023
pmc-release:
01
09
2024
medline:
11
9
2023
pubmed:
27
6
2023
entrez:
27
6
2023
Statut:
ppublish
Résumé
Nonhematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, the study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils and lymph nodes (LN), lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable nonhematopoietic cells. Flow cytometry and single-cell transcriptomics identified comparable proportions of LN stromal cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and LN. The presence and spatial distribution of transcriptionally defined cell types were confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSCs in human disease.
Identifiants
pubmed: 37366295
doi: 10.1002/eji.202250362
pmc: PMC10529925
mid: NIHMS1918857
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2250362Subventions
Organisme : NHLBI NIH HHS
ID : T32 HL007439
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI091627
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009140
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141408
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 Wiley-VCH GmbH.
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